An adapted duck Tembusu virus induces systemic infection and mediates antibody-dependent disease severity in mice

Liu, Zongliang; Ji, Yanhong; Huang, Xiaohui; Fu, Yue; Wei, Jinbei; Xue-peng, Cai; Zhu, Qing

doi:10.1016/j.virusres.2013.06.010

Cited by 24 publications

(19 citation statements)

References 27 publications

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“…Duck is the typical host of DTMUV, and some other species, like chickens, geese, and sparrows, also have been reported to be infected with DTMUV (Yu et al, 2018). Seriously, there might be high risk for poultry-to-human or mice transmission of the DTMUV (Liu et al, 2013;Tang et al, 2013). Therefore, it is emergency to study the DTMUV-host interaction and develop effective antivirus therapies.…”

Section: Introductionmentioning

confidence: 99%

Autophagy Is a Potential Therapeutic Target Against Duck Tembusu Virus Infection in vivo

Pan

Cheng

et al. 2020

Front. Cell. Infect. Microbiol.

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Duck tembusu virus (DTMUV) is newly emerged in poultry and causes great losses to the breeding industry in China and neighboring countries. Effective antiviral strategies are still being studied. Autophagy is a cellular degradative pathway, and our lab's previous data show that autophagy promotes DTMUV replication in vitro. To study the role of autophagy further in vivo, we utilized ducks as the animal model to investigate the autophagy responses in DTMUV-targeted tissues. And also, we utilized autophagy regulators, including Rapamycin (Rapa) as the autophagy enhancer, 3-Methyladenine (3-MA) and Chloroquine (CQ) as the autophagy inhibitors, to adjust the host autophagic levels and then study the effects of autophagy on tissue damages and virus replication. As a result, we first found DTMUV infection trigged autophagy and autophagy regulator treatments regulated autophagy levels successfully in duck spleens and brains. Next, we found that autophagy inhibitors inhibited DTMUV replication and alleviated DTMUV-induced pathological symptoms, whereas the autophagy inducer treatment led to the opposite effects. And we also found that autophagic regulation was correlated with the expression of innate immune genes, including pattern recognition receptors, type I interferons, and cytokines, and caused different effects in different tissues. In summary, we demonstrated that autophagy facilitated DTMUV replication, aggravated the developments of pathological symptoms and possibly counteracts the host's innate immunity response in vivo.

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Section: Introductionmentioning

confidence: 99%

Autophagy Is a Potential Therapeutic Target Against Duck Tembusu Virus Infection in vivo

Pan

Cheng

et al. 2020

Front. Cell. Infect. Microbiol.

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“…A previous study reported that duck TMUV induced high neurovirulence in intracerebrally inoculated BALB/c mice [9]. Cell-adapted duck TMUV replicates well in most organs of mice [10]. Moreover, TMUV antibodies and RNA have been detected in the serum and from oral swabs obtained from duck farm workers [11].…”

Section: Introductionmentioning

confidence: 99%

The Sequential Tissue Distribution of Duck Tembusu Virus in Adult Ducks

Liu

Chen

et al. 2014

BioMed Research International

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In 2010, a novel Tembusu virus (TMUV) that caused a severe decrease in the egg production of ducks was isolated in southeast China. Given the novelty of this duck pathogen, little information is available regarding its pathogenesis. Here, we systematically investigated the replication kinetics of TMUV PTD2010 in adult male and female ducks. We found that PTD2010 was detectable in most of the parenchymatous organs as well as the oviduct and intestinal tract from days 1 to 7 after inoculation. Viral titers were maintained at high levels for at least 9 days in the spleen, kidney, bursa of Fabricius, brain, and ovary. No virus was detected in any of these organs or tissues at 18 days after inoculation. PTD2010, thus, causes systemic infections in male and female ducks; its replication kinetics show similar patterns in most organs, with the exception of the ovaries and testes.

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“…We found that DTMUV is a poor inducer of IFN-b in HEK293 cells, even though its RNA can initiate and activate the RLR signaling pathway from 36 h postinfection. Moreover, we can assume that this effect is not cell specific because our current and previous studies indicated that DTMUV infection induced IFN production at the mRNA level in various cells at 36 h postinfection (37). These observations suggest that the inhibition of type I IFN activation by DTMUV might be a strategy for early evasion and to facilitate its pathogenesis and transmission.…”

Section: Discussionmentioning

confidence: 73%

“…Viruses, cell lines, and Abs DTMUV isolated from a dead duckling in Anhui Province in China was used in the study (37). Sendai virus (SeV) was kindly provided by Prof. H. Deng (Peking University, Peking, China).…”

Section: Methodsmentioning

confidence: 99%

Duck Tembusu Virus Nonstructural Protein 1 Antagonizes IFN-β Signaling Pathways by Targeting VISA

Wang

Lei

et al. 2016

The Journal of Immunology

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Duck Tembusu virus (DTMUV) is an emergent infectious pathogen that has caused severe disease in ducks and huge economic losses to the poultry industry in China since 2009. Previously, we showed that DTMUV inhibits IFN-β induction early in infection; however, the mechanisms of the inhibition of innate immune responses remain poorly understood. In this study, we screened DTMUV-encoded structural and nonstructural proteins using reporter assays and found that DTMUV NS1 markedly suppressed virus-triggered IFN-β expression by inhibiting retinoic acid-inducible gene I-like receptor signaling. Moreover, we found that DTMUV NS1 specifically interacted with the C-terminal domain of virus-induced signaling adaptor and impaired the association of retinoic acid-inducible gene I or melanoma differentiation-associated gene 5 and virus-induced signaling adaptor, thereby downregulating the retinoic acid-inducible gene I-like receptor-mediated signal transduction and cellular antiviral responses, leading to evasion of the innate immune response. Together, our findings reveal a novel mechanism manipulated by DTMUV to circumvent the host antiviral immune response.

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An adapted duck Tembusu virus induces systemic infection and mediates antibody-dependent disease severity in mice

Cited by 24 publications

References 27 publications

Autophagy Is a Potential Therapeutic Target Against Duck Tembusu Virus Infection in vivo

Autophagy Is a Potential Therapeutic Target Against Duck Tembusu Virus Infection in vivo

The Sequential Tissue Distribution of Duck Tembusu Virus in Adult Ducks

Duck Tembusu Virus Nonstructural Protein 1 Antagonizes IFN-β Signaling Pathways by Targeting VISA

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