An actomyosin network organizes niche morphology and responds to feedback from recruited stem cells

Warder, Bailey N.; Nelson, Kara A.; Sui, Justin; Anllo, Lauren; DiNardo, Stephen

doi:10.1101/2023.09.08.556877

Cited by 4 publications

(2 citation statements)

References 77 publications

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“…The fibroblasts are recruited into a condensate via directed migration, and then the condensate is segregated deeper into the skin via epidermal invagination driven by reciprocal signaling between dermis and epidermis (Biggs et al, 2018). The Drosophila testis niche cells require FGF and Slit signals from Vm to migrate through the testis and assemble as a cap that is further compacted via actomyosin contractility (Anllo & DiNardo, 2022; Warder et al, 2024). Together with our findings on PSC formation, a paradigm for niche formation is emerging that, subsequent to niche cell specification, extrinsic input segregates niche cells from other constituents, and positions the collective toward one end of the tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Though we have yet linked the precise architecture of the embryonic PSC to function, the specific architectures of multiple other niches have been shown to be functionally relevant. In the Drosophila gonad, a coalesced and compact niche is necessary for proper germline stem cell maintenance, niche signaling, and for orienting characteristically orthogonal germline stem cell divisions (Anllo & DiNardo, 2022; Warder et al, 2024). In the mammalian hair follicle, niche position determines stem cell fate (Rompolas et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

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TheDrosophilahematopoietic niche assembles through collective cell migration controlled by neighbor tissues and Slit-Robo signaling

Nelson,

Lenhart,

Anllo

et al. 2024

Preprint

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Niches are often found in specific positions in tissues relative to the stem cells they support. Consistency of niche position suggests that placement is important for niche function. However, the complexity of most niches has precluded a thorough understanding of how their proper placement is established. To address this, we investigated the formation of a genetically tractable niche, theDrosophilaPosterior Signaling Center (PSC), the assembly of which had not been previously explored. This niche controls hematopoietic progenitors of the lymph gland (LG). PSC cells were previously shown to be specified laterally in the embryo, but ultimately reside dorsally, at the LG posterior. Here, using live-imaging, we show that PSC cells migrate as a tight collective and associate with multiple tissues during their trajectory to the LG posterior. We find that Slit emanating from two extrinsic sources, visceral mesoderm and cardioblasts, is required for the PSC to remain a collective, and for its attachment to cardioblasts during migration. Without proper Slit-Robo signaling, PSC cells disperse, form aberrant contacts, and ultimately fail to reach their stereotypical position near progenitors. Our work characterizes a novel example of niche formation and identifies an extrinsic signaling relay that controls precise niche positioning.Impact StatementA ne w example of niche formation is provided which reveals the mode of niche cell migration, implicates extrinsic sources that deliver positional information, and uncovers the signaling pathway required for the precise, stereotypical positioning of the niche.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

TheDrosophilahematopoietic niche assembles through collective cell migration controlled by neighbor tissues and Slit-Robo signaling

Nelson,

Lenhart,

Anllo

et al. 2024

Preprint

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Niche cytoskeletal architecture is required for proper stem cell signaling and oriented division in theDrosophilatestis

Vida,

Botto,

DiNardo

2024

Preprint

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Stem cells are critical to repair and regenerate tissues, and often reside in a niche that controls their behavior. TheDrosophilatestis niche has been a paradigm for niche-stem cell interactions and is used here to address the cell biological features that maintain niche structure and function during its steady-state operation. We report enrichment of the Myosin II (MyoII) and a key regulator of acto-myosin contractility (AMC), Rho Kinase (ROK), within the niche cell cortex at the interface with germline stem cells (GSCs). Compromising MyoII and ROK disrupts niche architecture, suggesting that AMC in niche cells is important to maintain the reproducible structure of this niche. Furthermore, defects in niche architecture cause changes in stem cell function. Our data suggest that the niche signals less robustly to adjacent germ cells, yet the disrupted structure permits increased numbers of cells to respond to the signal. Finally, compromising Myo II in niche cells leads to an increase in mis-oriented centrosomes in GSCs as well as defects in the centrosome orientation checkpoint. Ultimately, this work identifies a critical role for AMC-dependent maintenance of niche structure to ensure a proper complement of stem cells with correct execution of stem cell divisions.Summary statementActomyosin contractility regulated niche architecture is critical for proper signaling and oriented division of germline stem cells.

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The Drosophila hematopoietic niche assembles through collective cell migration controlled by neighbor tissues and Slit-Robo signaling

Nelson,

Lenhart,

Anllo

et al. 2024

Preprint

View full text Add to dashboard Cite

Niches are often found in specific positions in tissues relative to the stem cells they support. Consistency of niche position suggests that placement is important for niche function. However, the complexity of most niches has precluded a thorough understanding of how their proper placement is established. To address this, we investigated the formation of a genetically tractable niche, the Drosophila Posterior Signaling Center (PSC), the assembly of which had not been previously explored. This niche controls hematopoietic progenitors of the lymph gland (LG). PSC cells were previously shown to be specified laterally in the embryo, but ultimately reside dorsally, at the LG posterior. Here, using live-imaging, we show that PSC cells migrate as a tight collective and associate with multiple tissues during their trajectory to the LG posterior. We find that Slit emanating from two extrinsic sources, visceral mesoderm and cardioblasts, is required for the PSC to remain a collective, and for its attachment to cardioblasts during migration. Without proper Slit-Robo signaling, PSC cells disperse, form aberrant contacts, and ultimately fail to reach their stereotypical position near progenitors. Our work characterizes a novel example of niche formation and identifies an extrinsic signaling relay that controls precise niche positioning.

show abstract

An actomyosin network organizes niche morphology and responds to feedback from recruited stem cells

Cited by 4 publications

References 77 publications

TheDrosophilahematopoietic niche assembles through collective cell migration controlled by neighbor tissues and Slit-Robo signaling

TheDrosophilahematopoietic niche assembles through collective cell migration controlled by neighbor tissues and Slit-Robo signaling

Niche cytoskeletal architecture is required for proper stem cell signaling and oriented division in theDrosophilatestis

The Drosophila hematopoietic niche assembles through collective cell migration controlled by neighbor tissues and Slit-Robo signaling

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