An active DNA vaccine against infectious pancreatic necrosis virus (IPNV) with a different mode of action than fish rhabdovirus DNA vaccines

Cuesta, Alberto; Chaves-Pozo, Elena; Heras, Ana Isabel de las; Saint-Jean, Sylvia Rodríguez; Pérez-Prieto, Sara I.; Tafalla, Carolina

doi:10.1016/j.vaccine.2010.02.106

Cited by 50 publications

(30 citation statements)

References 32 publications

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“…In other studies, VHSV infection increased the trout NKEF and CD8 gene in vivo but failed to modulate the NKEF, perforin and granzyme genes in vitro (Cuesta & Tafalla, 2009;Ordás et al, 2011). VHSV and IPNV DNA vaccination also up-regulated the trout CD8, perforin and granzyme gene expression (Cuesta et al, 2010;unpublished data), giving more consistency to the involvement of CMC activity against viral infections and its activation by DNA vaccines. Moreover, oral vaccination with inactivated CHNV elicited specific CMC activity that resulted viral antigen-specific and restricted to the MHC I (Sato & Okamoto, 2010).…”

Section: Cmc Activity Referencesmentioning

confidence: 78%

“…The transcript level of CD8 gene is related to the CTL presence, abundance and activity. Thus, fish CD8 transcripts are up-regulated by viral and bacterial infections, viral DNA vaccines, scale grafts, poly I:C or mitogens (Byon et al, 2005(Byon et al, 2006Cuesta et al, 2010;Cuesta & Tafalla, 2009;Forlenza et al, 2008;Overturf & LaPatra, 2006;Somamoto et al, 2005Somamoto et al, , 2006Utke et al, 2007;Xu et al, 2011). In some studies, these CD8 gene levels have been correlated with increased CTL activity.…”

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 99%

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Fighting Virus and Parasites with Fish Cytotoxic Cells

Esteban¹,

Meseguer²,

Cuesta³

2012

Health and Environment in Aquaculture

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Section: Cmc Activity Referencesmentioning

confidence: 78%

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 99%

Fighting Virus and Parasites with Fish Cytotoxic Cells

Esteban¹,

Meseguer²,

Cuesta³

2012

Health and Environment in Aquaculture

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“…Encapsulation has resulted in obtaining microparticles smaller than those of bacteria (10 um), which allows for their incorporation and DNA expression in organs such as spleen, kidney, liver, pyloric caeca and stomach for months after the vaccination by depositing microparticles in the stomach using stents (De Las Heras et al, 2010;. The period of protection is much longer than administration of naked DNA is administered Zheng et al, 2006).…”

Section: Viral Antigens Encapsulated In Alginatementioning

confidence: 99%

“…However, this humoral response often does not correspond to the level of protection provided by the vaccine (Cuesta et al, 2010;De Las Heras et al, 2010;Dorson et al, 1978;Gomez-Casado et al, 2011;Koellner y Kotterba 2002). The increased expression or the synthesis of some molecules associated with stimulating innate immune response in fi sh such as some infl ammatory cytokines, interferon (IFN) and associated molecules is also currently used as a measure of the effi cacy of vaccines (Walden Villarino et al, 2010;Pettersen et al, 2008;Vallejo et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines

2013

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Aquaculture has become an important economic sector worldwide, but is faced with an ongoing threat from infectious diseases. Vaccination plays a critical role in protecting commercially raised fi sh from bacterial, viral and parasitic diseases. However, the production of eff ective vaccines is limited by the scarcity of knowledge about the immune system of fi sh. Improving vaccines implies using antigens, adjuvants and employing methods of administration that are more eff ective and less harmful to the fi sh. In this context, in recent year there have studies of methods of encapsulating antigens in matrices of diff erent types to apply in fi sh vaccines. This work reviews the new methods to improve fi sh vaccines by encapsulating them in polymers and polysaccharides.

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“…Significant protection levels have also been achieved with DNA vaccines constructed for other fish viruses [5][6][7]. Concerning IHNV and VHSV vaccination in rainbow trout (Oncorhynchus mykiss), a single intramuscular (i.m.)…”

Section: Introductionmentioning

confidence: 99%

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

Castro¹,

Martinez-Alonso²,

Fischer³

et al. 2014

Vaccine

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a b s t r a c tIn fish, intramuscular (i.m) injection of plasmid DNA encoding viral proteins has proved a highly effective vaccination strategy against some viral pathogens. The efficacy of DNA vaccination in teleost fish is based on the high level of viral antigen expression in muscle cells inducing a strong and long-lasting protection. However, the mechanisms through which this protection is established and effectuated in fish are still not fully understood. Moreover, similarities to mammalian models cannot be established since DNA vaccination in mammals usually induces much weaker responses. In this work, we have focused on the characterization of the immune cells that infiltrate the muscle at the site of DNA injection in vaccinated fish and the chemokines and chemokine receptors that may be involved in their infiltration. We have demonstrated through diverse techniques that B lymphocytes, both IgM + and IgT + cells, represented a major infiltrating cell type in fish vaccinated with a viral haemorrhagic septicaemia virus (VHSV) glycoprotein-encoding DNA vaccine, whereas in control fish injected with an oil adjuvant mainly granulocyte/monocyte-type cells were attracted. Among twelve chemokine genes studied, only CXCL11 L1, CK5B and CK6 mRNA levels were up-regulated in DNA vaccinated fish compared to fish injected with the corresponding vector backbone. Furthermore, the transcription of CXCR3B, a possible receptor for CXCL11 L1 was also significantly up-regulated in vaccinated fish. Finally, experiments performed with recombinant trout CK5B and CK6 and chemokine expression plasmids revealed that these chemokines have chemotactic capacities which might explain the recruitment of B cells to the site of DNA injection. Altogether, our results reveal that there is an early chemokine-related B cell recruitment triggered by i.m. DNA vaccination against VHSV which might play an important role in the initial phase of the immune response.

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An active DNA vaccine against infectious pancreatic necrosis virus (IPNV) with a different mode of action than fish rhabdovirus DNA vaccines

Cited by 50 publications

References 32 publications

Fighting Virus and Parasites with Fish Cytotoxic Cells

Fighting Virus and Parasites with Fish Cytotoxic Cells

Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

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