An actin–myosin complex on actively transcribing genes

Fomproix, Nathalie; Percipalle, Piergiorgio

doi:10.1016/j.yexcr.2003.10.028

Cited by 177 publications

(205 citation statements)

References 24 publications

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“…The presence of ␤-actin in the nucleus was documented for the first time many years ago (Egly et al, 1984;Scheer et al, 1984), but the connection between ␤-actin and gene transcription has only recently been demonstrated (Olave et al, 2002;Pederson and Aebi, 2002;Fomproix and Percipalle, 2004;Grummt, 2006;Jockusch et al, 2006;Percipalle and Visa, 2006;Obrdlik et al, 2007). Nuclear ␤-actin associates with components of the ATP-dependent chromatin-remodeling complexes (Olave et al, 2002;Bettinger et al, 2004), with RNP particles (Percipalle and Visa, 2006), and with the three RNA polymerases in the eukaryotic cell nucleus, both in vitro and in vivo (Hu et al, 2004;Philimonenko et al, 2004;Kukalev et al, 2005;Wu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization

Ferrai

Naum-Onganía

Longobardi

et al. 2009

MBoC

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We have analyzed the role of actin polymerization in retinoic acid (RA)-induced HoxB transcription, which is mediated by the HoxB regulator Prep1. RA induction of the HoxB genes can be prevented by the inhibition of actin polymerization. Importantly, inhibition of actin polymerization specifically affects the transcription of inducible Hox genes, but not that of their transcriptional regulators, the RARs, nor of constitutively expressed, nor of actively transcribed Hox genes. RA treatment induces the recruitment to the HoxB2 gene enhancer of a complex composed of "elongating" RNAPII, Prep1, ␤-actin, and N-WASP as well as the accessory splicing components p54Nrb and PSF. We show that inhibition of actin polymerization prevents such recruitment. We conclude that inducible Hox genes are selectively sensitive to the inhibition of actin polymerization and that actin polymerization is required for the assembly of a transcription complex on the regulatory region of the Hox genes.

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Section: Introductionmentioning

confidence: 99%

Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization

Ferrai

Naum-Onganía

Longobardi

et al. 2009

MBoC

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“…In addition, ␤ actin has been shown to be necessary for the activity of partially purified RNA polymerase III (33). The role of actin in transcription is further supported by the findings that actin is associated with all three RNA polymerases (29,(31)(32)(33)(34). Despite this progress, one important unsolved issue is whether actin polymerization is also essential for the various nuclear functions of actin, including regulation of transcription, although several recent studies suggest the presence of polymeric actins in the nucleus (35,36).…”

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confidence: 96%

A Novel Role of the Actin-nucleating Arp2/3 Complex in the Regulation of RNA Polymerase II-dependent Transcription

Yoo

Guan

2007

Journal of Biological Chemistry

119

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It has been well documented that actin is present in the nucleus and involved in numerous nuclear functions including regulation of transcription. The actin-nucleating Arp2/3 complex is an essential, evolutionarily conserved seven-subunit protein complex that promotes actin cytoskeleton assembly in the cytoplasm upon stimulation by WASP family proteins. Our recent study indicates that the nuclear localized neural Wiskott-Aldrich syndrome protein (N-WASP) can induce de novo actin polymerization in the nucleus, and this function is important for the role of N-WASP in the regulation of RNA polymerase II-dependent transcription. Here, we have presented evidence to show that the Arp2/3 complex is also localized in the nucleus and plays an essential role in mediating nuclear actin polymerization induced by N-WASP. We have also demonstrated that the Arp2/3 complex physically associates with RNA polymerase II and is involved in the RNA polymerase II-dependent transcriptional regulation both in vivo and in vitro. Together, these data provide strong support for the hypothesis that N-WASP and the Arp2/3 complex regulate transcription, at least in part, through the regulation of nuclear actin polymerization in a manner similar to their function in the cytoplasm.

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“…161,162 An actin-myosin complex associated with RNA polymerase I was described in nucleoli and functionally coupled to elongating transcripts in HeLa cells. 163 Here, an actin-based myosin motor was described to be associated with transcribing ribosomal genes in the nucleus. It was suggested that actin-myosin motors might provide a general mechanism to facilitate elongation of RNA transcripts during transcription of both ribosomal genes and protein-coding genes.…”

Section: Actin Involved In Transcriptionmentioning

confidence: 99%

Signal Transduction and Actin in the Regulation of G1-Phase Progression

Boonstra

Moes

2005

Crit Rev Eukar Gene Expr

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Regulation of cell proliferation is dependent on the integration of signal transduction systems that are activated by external signal molecules, such as growth factors and extracellular matrix components. Dependent on these signal transduction networks, the cells decide in the G1 phase to continue proliferation or, alternatively, to stop cell-cycle progression and undergo apoptosis, differentiation, or quiescence. The MAP kinase and PI-3 kinase pathways have been demonstrated to play an essential role in these G1-phase decisions. Interestingly, actin has been demonstrated to mutually interfere with signal transduction. In addition, it has been indicated that the FOXO transcription factors are involved in these decisions, as well. Actin has been demonstrated to play an important role in the regulation of G1-phase progression. Because of its properties as a structural protein, actin is essential in cytokinesis and in cell spreading and, thus, is involved in G1-phase progression. As an intermediate factor in signal transduction, actin is likely to be involved in cell-cycle regulation induced by external signal molecules. And, finally, actin has been demonstrated to play a direct role in transcription. These observations indicate a prominent role of actin in the regulation of G1-phase progression.

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An actin–myosin complex on actively transcribing genes

Cited by 177 publications

References 24 publications

Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization

Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization

A Novel Role of the Actin-nucleating Arp2/3 Complex in the Regulation of RNA Polymerase II-dependent Transcription

Signal Transduction and Actin in the Regulation of G1-Phase Progression

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