An acetylcholinesterase enzyme electrode stabilized by an electrodeposited gold nanoparticle layer

Modified Cholinesterase Technology in the Construction of Biosensors for Organophosphorus Nerve Agents and Pesticides Detection

“…In case of cholinesterase biosensors it is possible to lower the voltage and interferences of electroactive sample components by means of redox transmitters such as ferophtalocyanine, cobalt phtalocyanine, Prussian blue (ferrihexacyanofferate), 7,7,8, in combination with carbon electrodes, screen electrodes or using the carbon nanotube (CNT) (Arduini et al, 2007;Ciucu et. al., 2003;Hart et al, 1997;Hernandez et al, 2000;Lin et al, 2004;Ricci et al, 2003;Ivanov et al, 2003a;Nunes et al, 2004;Shulga & Kirchhoff, 2007;Skládal, 1992;Skládal & Mascini, 1992;Suprun et al, 2005;Wring et al, 1989) or electrochemical decomposition of the arising peroxide using a gold or platinum amperometric electrode (Yao, 1983). CNTs are commonly used for electrochemical biosensors.…”

Enzyme-based Electrochemical Biosensors

“…The electrode surface could be roughened by gold nanoparticles to enhance the interaction of enzyme with the electrode. An example is the construction of acetylcholinesterase biosensor in which electrode was modified by electrodeposited gold nanoparticles at the electrode surface after hydrolysis of acethlthiocholine by the immobilization enzyme (Shulga & Kirchhoff, 2007). This method is valuable for the development of new devices for the sensitive detection of potentially dangerous and deadly neurotoxins.…”

Functional and Selective Bacterial Interfaces Using Cross-Scaffold Gold Binding Peptides

“…[1][2][3][4][5] In particular, the conjugation of biomolecules (proteins, enzymes, deoxyribonucleic acid (DNA), and lipids) as well as living cells to both flat and structured gold has been extensively documented. [6][7][8][9][10][11][12][13][14][15][16] Traditionally, biomolecule coupling to gold (or other substrates) is often facilitated by a cross-linking agent and rarely directly to the gold surface itself. Integration of a gold-binding peptide into a biomolecule of interest allows utilization of more complex chemistry and offers the advantage of direct integration with the surface.…”