An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study

Tsujimoto, Takeru; Sudo, Hideki; Todoh, Masahiro; Yamada, Kei; Iwasaki, Koji; Ohnishi, Takashi; Hirohama, Naoki; Nonoyama, Takayuki; Ukeba, Daisuke; Ura, Katsuro; Ito, Yoichi M.; Iwasaki, Norimasa

doi:10.1016/j.ebiom.2018.10.055

Cited by 54 publications

(184 citation statements)

References 62 publications

(132 reference statements)

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“…Thus, it might be free from potential animal pathogens. Furthermore, in vivo biological safety tests based on an international organization for standardization (ISO) and good laboratory practice standards showed that UPAL does not have immunogenicity to the human body 31 . In addition, the UPAL group provided more CD31-and HNF4α-positive cells than any other groups, illustrating that UPAL coating not only increased the engraftment area but also increased the expression of vascular endothelial cells and hepatocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Compared with other coating agents, UPAL yielded the best effect in improving transplant tissue survival rate and replenishing the liver function. Moreover, the intradiscal injection of UPAL gel can suppress lumbar intervertebral disc degeneration and promote the production of extracellular matrix, essential for nucleus pulposus cell function after discectomy 31 .…”

Section: Discussionmentioning

confidence: 99%

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Transplantation of fetal liver tissue coated by ultra-purified alginate gel over liver improves hepatic function in the cirrhosis rat model

Qiu

Murata

Oshiro

et al. 2020

Sci Rep

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In this study, we used a new coating agent, that is, ultra-purified alginate gel (UPAL), for fetal liver tissue transplantation. This study aims to compare the effect of UPAL with the effect of other coating agents on improving the effect of fetal liver tissue transplantation in a liver cirrhosis rat model. Prior to the transplantation of wild-type ED14 fetal liver tissues, various coating agents were separately applied on the liver surface of rats with cirrhosis. Then, we compared the engraftment area, engraftment rate and liver function level of these rats. As a result, coating the liver surface of a cirrhosis rat with UPAL obtained the best effect in terms of engraftment area and engraftment rate of the transplanted liver tissue and in the recovery of liver function compared with control group. Therefore, UPAL coating may serve as a novel strategy for liver organoid transplantation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transplantation of fetal liver tissue coated by ultra-purified alginate gel over liver improves hepatic function in the cirrhosis rat model

Qiu

Murata

Oshiro

et al. 2020

Sci Rep

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“…The Pfirrmann grading score was used for quantitative analysis of degenerative changes in rabbit lumbar IVDs. Alterations in NP were quantified using relative MRI index, as described previously . No significant difference was detected in the Pfirrmann grading score and relative MRI index among all groups at 6 or 12 months (Fig.…”

Section: Resultsmentioning

confidence: 95%

“…Quantitative analysis of T2‐weighted, mid‐sagittal image slices was also processed using the Analyze 10.0 software (AnalyzeDirect, Overland Park, KS), as reported previously . The MRI index (product of NP area and average signal intensity) was used for the quantification of alterations in NP, and quantitative data were expressed as percentage relative to the MRI index obtained with untreated control disks (relative MRI index) . All image assessments were performed by two independent blind observers, and the quantitative data were presented as the mean of the three evaluations ( n = 8 disks for each treatment group/time point) …”

Section: Methodsmentioning

confidence: 99%

Effects of Intradiscal Injection of Local Anesthetics on Intervertebral Disc Degeneration in Rabbit Degenerated Intervertebral Disc

Ura

Sudo

Iwasaki

et al. 2019

Journal Orthopaedic Research

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Analgesic discoblock is widely used for the diagnosis or treatment of discogenic low back pain by injecting local anesthetics. The purpose of this study was to investigate the deleterious effects of local anesthetics on degenerated rabbit intervertebral disks (IVDs) using an organotypic culture model and in vivo long-term follow-up model. To induce IVD degeneration, a rabbit annular puncture model was used. For the organotypic culture model, degenerated IVDs were harvested 1 month after the initial annular puncture and cultured for 3 or 7 days after intradiscal injection of local anesthetics (1% lidocaine and 0.5% bupivacaine). To perform in vivo analysis, local anesthetics were injected into degenerated IVDs, and IVDs were prepared for histological analysis after 6 or 12 months. In the organotypic model, terminal deoxynucleotidyl transferase dUTP nick end labeling-positive nucleus pulposus (NP) cells were significantly increased in the bupivacaine group compared with the other groups. In the in vivo study, the number of NP cells was significantly decreased in the saline and local anesthetics groups compared with the untreated control and puncture-only groups. However, there was no significant difference among the saline, lidocaine, and bupivacaine groups. In addition, histological analysis showed no significant difference of IVD degeneration among the puncture-only, saline, lidocaine, and bupivacaine groups. Although bupivacaine induced apoptotic NP cell death in the organotypic culture model, in vivo observations did not show any definitive proof to suggest that local anesthetics were capable of promoting degeneration in the degenerated IVD, except for pressurized injection-induced damage.

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“…Alginate gels have been extensively studied for tissue engineering applications as cell encapsulation material as well as for in vivo cell delivery as an injectable 3D matrix. Several studies demonstrated that calcium alginate gel, although easy to obtain, exhibited poor biodegradation, bioresorbability and cell adhesion [ 112 ]. For example, ultrapurified alginate gels are biocompatible with human lumbar intervertebral disc cells and promote extracellular matrix production after discectomy.…”

Section: Applications Of Alginate In the Medical Fieldmentioning

confidence: 99%

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

et al. 2020

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Initially used extensively as an additive and ingredient in the food industry, alginate has become an important compound for a wide range of industries and applications, such as the medical, pharmaceutical and cosmetics sectors. In the food industry, alginate has been used to coat fruits and vegetables, as a microbial and viral protection product, and as a gelling, thickening, stabilizing or emulsifying agent. Its biocompatibility, biodegradability, nontoxicity and the possibility of it being used in quantum satis doses prompted scientists to explore new properties for alginate usage. Thus, the use of alginate has been expanded so as to be directed towards the pharmaceutical and biomedical industries, where studies have shown that it can be used successfully as biomaterial for wound, hydrogel, and aerogel dressings, among others. Furthermore, the ability to encapsulate natural substances has led to the possibility of using alginate as a drug coating and drug delivery agent, including the encapsulation of probiotics. This is important considering the fact that, until recently, encapsulation and coating agents used in the pharmaceutical industry were limited to the use of lactose, a potentially allergenic agent or gelatin. Obtained at a relatively low cost from marine brown algae, this hydrocolloid can also be used as a potential tool in the management of diabetes, not only as an insulin delivery agent but also due to its ability to improve insulin resistance, attenuate chronic inflammation and decrease oxidative stress. In addition, alginate has been recognized as a potential weight loss treatment, as alginate supplementation has been used as an adjunct treatment to energy restriction, to enhance satiety and improve weight loss in obese individuals. Thus, alginate holds the promise of an effective product used in the food industry as well as in the management of metabolic disorders such as diabetes and obesity. This review highlights recent research advances on the characteristics of alginate and brings to the forefront the beneficial aspects of using alginate, from the food industry to the biomedical field.

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An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study

Cited by 54 publications

References 62 publications

Transplantation of fetal liver tissue coated by ultra-purified alginate gel over liver improves hepatic function in the cirrhosis rat model

Transplantation of fetal liver tissue coated by ultra-purified alginate gel over liver improves hepatic function in the cirrhosis rat model

Effects of Intradiscal Injection of Local Anesthetics on Intervertebral Disc Degeneration in Rabbit Degenerated Intervertebral Disc

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

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