An accurate identification of Pythium insidiosum complex using MALDI-TOF, requires representative spectra of each of its four phylogenetic clusters

Mendoza, Leonel; Vilela, Raquel; Mani, Rinosh

doi:10.1016/j.ijid.2019.07.025

Cited by 2 publications

(2 citation statements)

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“…53 It is thus important to evaluate the spectra of each of the different phylogenetic clusters for an accurate identification of the P insidiosum complex. [53][54][55] Even so, the paraphyletic status of P insidiosum Thai isolates in relation to American ones support the scenario in which American isolates or species derive from Asian ones. 12 In this case, it seems plausible that the Great American Interchange played a role in the geodispersal of P insidiosum about 3-2.6 Mya.…”

Section: And Mccarthymentioning

confidence: 87%

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New insights on evolutionary aspects of Pythium insidiosum and other peronosporaleans

Weiblen

Robe

Azevedo

et al. 2020

Mycoses

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Summary Background The evolution of pathogenic mechanisms is a major challenge, which requires a thorough comprehension of the phylogenetic relationships of pathogens. Peronosporaleans encompasses a heterogeneous group of oomycetes that includes some animal/human pathogens, like Pythium insidiosum. Objective We analysed here the phylogenetic positioning and other evolutionary aspects related to this species and other peronosporaleans, using a multi‐locus approach with one mitochondrial and three nuclear genes. Methodology Phylogenetic patterns of 55 oomycetes were inferred by maximum likelihood and Bayesian analysis, and a relaxed molecular clock method was applied to infer the divergence time of some peronosporaleans branches. Results Pythium insidiosum was monophyletic with a major and polytomous clade of American isolates; however, Pythium spp. was found to be paraphyletic with Phytopythium sp. and Phytophthora spp. In general, peronosporaleans subdivided into four lineages, one of which evidenced a close relationship of P insidiosum, P aphanidermatum and P arrhenomanes. This lineage diverged about 63 million years ago (Mya), whereas P insidiosum diversified at approximately 24 Mya. The divergence of American and Thai isolates seems to have occurred at approximately 17 Mya, with further American diversification at 2.4 Mya. Conclusion Overall, this study clarifies the phylogenetic relationships of P insidiosum regarding other peronosporaleans in a multi‐locus perspective, despite previous claims that phylogenomic analyses are needed to accurately infer the patterns and processes related to the evolution of different lineages in this group. Additionally, this is the first time that a molecular clock was applied to study the evolution of P insidiosum.

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Section: And Mccarthymentioning

confidence: 87%

“…and Phytopythium litorale , respectively . It is thus important to evaluate the spectra of each of the different phylogenetic clusters for an accurate identification of the P insidiosum complex …”

Section: Discussionmentioning

confidence: 99%