Biosemiconductors are highly efficient systems for converting solar energy into chemical energy. However, the inevitable presence of reactive oxygen species (ROS) seriously deteriorates the biosemiconductor performance. This work successfully constructed a Mn 3 O 4 nanozyme-coated biosemiconductor, Thiobacillus denitrificans-cadmium sulfide (T. denitrificans-CdS@Mn 3 O 4 ), via a simple, fast, and economic method. After Mn 3 O 4 coating, the ROS were greatly eliminated; the concentrations of hydroxyl radicals, superoxide radicals, and hydrogen peroxide were reduced by 90%, 77.6%, and 26%, respectively, during photoelectrotrophic denitrification (PEDeN). T. denitrificans-CdS@Mn 3 O 4 showed a 28% higher rate of nitrate reduction and 78% lower emission of nitrous oxide (at 68 h) than that of T. denitrificans-CdS. Moreover, the Mn 3 O 4 coating effectively maintained the microbial viability and photochemical activity of CdS in the biosemiconductor. Importantly, no lag period was observed during PEDeN, suggesting that the Mn 3 O 4 coating does not affect the metabolism of T. denitrificans-CdS. Immediate decomposition and physical separation are the two possible ways to protect a biosemiconductor from ROS damage by Mn 3 O 4 . This study provides a simple method for protecting biosemiconductors from the toxicity of inevitably generated ROS and will help develop more stable and efficient biosemiconductors in the future.