An accurate and transferable protocol for reproducible quantification of organic pollutants in human serum using direct isotope dilution mass spectrometry

Boggess, Andrew J.; Rahman, G. M. Mizanur; Pamukcu, M.; Faber, Scott; Kingston, H. M.

doi:10.1039/c4an00851k

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…12 This LOQ sufficiently exceeded the National Primary Drinking Water Regulations from the USEPA, which specied a maximum glyphosate contaminant level of 0.700 mg mL À1 . Method LOQ for ESI-TOF-MS using traditional SPE and IDMS quantication for glyphosate in drinking water was found to be 0.401 AE 0.01 mg mL À1 .…”

Section: Validation Of Spe and Esi-tof-msmentioning

confidence: 93%

“…Using IDMS, quantitative accuracy and precision was maintained as concentration values were decreased approaching method LOQ, conrming previous studies on molecular IDMS quantication. 12 This LOQ sufficiently exceeded the National Primary Drinking Water Regulations from the USEPA, which specied a maximum glyphosate contaminant level of 0.700 mg mL À1 .…”

Section: Validation Of Spe and Esi-tof-msmentioning

confidence: 93%

“…10 IDMS is a quantication technique involving the spiking of accurate amounts of isotopically labeled analogs into an unknown sample. 12 However, in-laboratory spiking of the isotopically labeled analogs into the unknown samples remains a potential source of analytical error when transferring developed methods using IDMS. IDMS can correct for many sources of error oen associated with extraction, mass spectrometry, and quantication.…”

Section: Introductionmentioning

confidence: 99%

“…10,11 By reducing the inuence of common error-introducing analytical steps on nal quantitative quality, IDMS is capable of transferring high accuracy methods between laboratories and analysts with minimal additional training. 12 However, in-laboratory spiking of the isotopically labeled analogs into the unknown samples remains a potential source of analytical error when transferring developed methods using IDMS.…”

Section: Introductionmentioning

confidence: 99%

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Sensitive and stable pre-calibrated solid-phase extraction columns for environmental and forensic quantification using isotope dilution mass spectrometry

et al. 2015

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A method was developed to pre-load solid-phase extraction columns (SPE) with isotopic calibrant for use with isotope dilution mass spectrometry (IDMS). The pre-calibration method was developed, optimized, and validated for the quantification of the pesticide glyphosate in drinking water using anion-exchange SPE, electrospray-ionization, and time-of-flight mass spectrometry (ESI-TOF-MS). The instrumental method obtained a mass-accuracy of 3 ppm and a limit of quantification (LOQ) of 0.3 ng mL À1 . Quantification of glyphosate by IDMS significantly improved quantitative error and LOQ compared with the calibration curve. The pre-loading methodology was optimized for stability over time and validated in drinking water, exhibiting an accuracy of 1.25% AE 0.87% error with no significant difference from certified concentrations or traditional SPE. Method LOQ was 0.4 ng mL À1 . Quantifying glyphosate in spiked drinking water sample produced high accuracy up to two-weeks after pre-loading columns, with an accuracy of 6.41% AE 7.10% error. A potential forensic application was investigated by adapting the pre-loading method to the quantification of seven drugs in synthetic urine using a mixed-mode SPE column. The ESI-TOF-MS method using traditional SPE produced accurate quantification of all seven drugs in synthetic urine with a mean error of 4.16% AE 3.07% and LOQ of 0.780 ng mL À1 . The pre-loading method produced accurate quantification with 5.36% AE 4.73% error, with no significant difference from traditional SPE or certified standards. Five of the seven drugs were quantified at high accuracy one week after pre-loading, with 5.40% AE 4.57% error from certified values. This method may be applicable to analysts seeking to develop methods to improve the transfer of high-accuracy and precision methods between laboratories.

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Section: Validation Of Spe and Esi-tof-msmentioning

confidence: 93%

Section: Validation Of Spe and Esi-tof-msmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sensitive and stable pre-calibrated solid-phase extraction columns for environmental and forensic quantification using isotope dilution mass spectrometry

et al. 2015

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“…All serum samples were assigned a randomized identification number and all analysts were blinded to the identity of samples. Serum samples were analyzed by previously peer-reviewed methods 36 . Chromatography and mass spectrometry were conducted using an Gas Chromatograph –Mass Spectrometer system.…”

Section: Methodsmentioning

confidence: 99%

Mean serum-level of common organic pollutants is predictive of behavioral severity in children with autism spectrum disorders

Boggess

Faber

Kern

et al. 2016

Sci Rep

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Autism spectrum disorders (ASD), and their pathogenesis, are growing public health concerns. This study evaluated common organic pollutant serum-concentrations in children, as it related to behavioral severity determined by rating scales and the Autism Diagnostic Observation Schedule (ADOS). Thirty children, ages 2–9, with ASD and thirty controls matched by age, sex, and socioeconomic status were evaluated using direct blood serum sampling and ADOS. Pooling concentrations of all studied pollutants into a single variable yielded cohort-specific neurobehavioral relationships. Pooled serum-concentration correlated significantly with increasing behavioral severity on the ADOS in the ASD cohort (p = 0.011, r = 0.54), but not controls (p = 0.60, r = 0.11). Logistic regression significantly correlated mean pollutant serum-concentration with the probability of diagnosis of behaviorally severe autism, defined as ADOS >14, across all participants (odds ratio = 3.43 [95% confidence: 1.14–10.4], p = 0.0287). No specific analyte correlated with ADOS in either cohort. The ASD cohort displayed greater quantitative variance of analyte concentrations than controls (p = 0.006), suggesting a wide range of detoxification functioning in the ASD cohort. This study supports the hypothesis that environmental exposure to organic pollutants may play a significant role in the behavioral presentation of autism.

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Dried blood spots for the identification of bioaccumulating organic compounds: Current challenges and future perspectives

Jobst

Arora

Pollitt

et al. 2020

Current Opinion in Environmental Science & Health

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An accurate and transferable protocol for reproducible quantification of organic pollutants in human serum using direct isotope dilution mass spectrometry

Abstract: A novel and transferable method for the accurate and direct quantification of organic pollutants in human serum.

Cited by 8 publications

References 30 publications

Sensitive and stable pre-calibrated solid-phase extraction columns for environmental and forensic quantification using isotope dilution mass spectrometry

Sensitive and stable pre-calibrated solid-phase extraction columns for environmental and forensic quantification using isotope dilution mass spectrometry

Mean serum-level of common organic pollutants is predictive of behavioral severity in children with autism spectrum disorders

Dried blood spots for the identification of bioaccumulating organic compounds: Current challenges and future perspectives

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