An accurate and rapid detection of lymph node metastasis in non-small cell lung cancer patients based on one-step nucleic acid amplification assay

Clinicopathological Characteristics of Lung Adenocarcinoma with Unexpected Lymph Node Metastasis

“…Ohno et al 18) evaluated the diagnostic capability of short inversion time inversion-recovery (STIR) turbo spin-echo (SE) imaging in nodal assessment in patients with NSCLC, and they concluded that STIR turbo SE imaging is more sensitive and/or more accurate than FDG-PET/CT. 18) Inoue et al 19) suggested the potential clinical utility of one-step nucleic acid amplification (OSNA) assay, which was an automated rapid molecular diagnostic method using optimal mRNA marker, for intraoperative rapid and accurate diagnosis of nodal status in lung cancer. However, if using these new diagnostic technologies, it would be quite difficult to detect micrometastatic foci of lymph nodes reliably.…”

Rapid detection of CEA mRNA in peritoneal washes using One-Step Nucleic acid Amplification (OSNA®) for gastric cancer patients

“…Previously, we and other researchers have reported a new rapid gene detection system, One-Step Nucleic acid Amplification (OSNA), for lymph node metastasis detection in breast, colorectal, gastric, and lung cancer patients [21][22][23][24]. This method consists of a simple assay sample preparation and automated rapid gene detection using a reverse transcription loop-mediated amplification (RT-LAMP) method [25].…”

Quantitative RT-PCR Assay for Detecting Lymph Node Metastasis in Endometrial Cancer: A Preliminary Study

“…However, metastatic LN were present in only 2 patients; this sample size was too small to analyze the efficacy of the method. In addition, numerous studies have shown that CK19 mRNA analysis achieves the best molecular diagnostic performance for LN metastasis [9, 12-16]. CK19 is not expressed in LN cells but is expressed in epithelial cells, including those of the female genital tract [17].…”