An abnormality of adrenaline, phentolamine stimulated lipolysis in adipose tissue from obese, maturity-onset diabetics

Jn, Atkinson; Randle, P. J.

doi:10.1007/bf01212161

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…In agreement with Atkinson and Randle (15) preliminary experiments indicated that phentolamine methane sulphonate alone had no effect on glycerol release from fat cells in concentrations up to 130 pM and that a concentration of 13 pM was sufficient for maximum enhancement of adrenaline stimulated lipolysis. Table 1 shows the amount of glycerol released during 2 hours incubation, in response to various concentrations of adrenaline in ehe presence of 13 pM phentolamine.…”

Section: The Release Of Glycerol In Fat Cells In Vitrosupporting

confidence: 84%

The Effect of Pregnancy on the Control of Lipolysis in Fat Cells Isolated from Human Adipose Tissue

Elliott¹

1975

Eur J Clin Investigation

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1 . Lipolysis has been estimated by measuring the release of g l y c h . in isolated adipose tissue cells obtained from women in early prognancy, late pregnancy and 1 -3 days post partwn and from non-pregnant women. 2 . Adipocytes of women at the end of pregnancy exhibited higher rates of lipolysis in response to adrenaline (1.5 -15 uM) plus phentolamine (13 uM) than those of non-pregnant women or those in early pregnancy. 3 . Lipolysis in response to adrenaline plus phentolamine in fat cells from women 1 -3 days post partwn was reduced compared to that at the end of gestation but was enhanced relative to that in the non-pregnant or early Pregnant state. 4 . Basal lipolysis also tended to be greatest at term. 5. Under conditions where the production of cyclic AMP was not rate limiting for the stimulation of lipolysis, that is in the presence of dibutyryl cyclic AMP ( I mM) or adrenaline (15 VM) plus phentolamine (13 pM) plus caffeine ( 1 mM), the release of glycerol in cells from women at term and in the puerperium was greater than that in women in the non-pregnant or early pregnant state. 6 . Cell levels of cyclic AMP rose after incubation with adrenaline ( 6 pM) plus phentolamine or adrenaline ( 1 5 pM) plus phentolamine plus caffeine ( 1 mM) but were similar in all four groups of women. 7 . It is concluded that the observed enhancement of lipolysis demonstrated.in fat cells from women at the end of pregnancy reflects an increase in hormonesensitive lipase activity rather than a modification of hormone receptor site sensitivity or of the rates of synthesis or breakdown of cyclic AMP. 8. This increase in adipose tissue lipolysis at the end of gestation could contribute to the reported rise in plasma nonesterified fatty acids in the final weeks of pregnancy.

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Section: The Release Of Glycerol In Fat Cells In Vitrosupporting

confidence: 84%

The Effect of Pregnancy on the Control of Lipolysis in Fat Cells Isolated from Human Adipose Tissue

Elliott¹

1975

Eur J Clin Investigation

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“…It is therefore unlikely that changes in catecholamine receptor sensitivity are responsible for the increased flux of plasma fatty acids that is frequently observed in adult diabetes. A number of reasons may account for the discrepancy between our results and those of Atkinson and Randle [5]. The severity of diabetes mellitus at time of study, [19].…”

Section: Discussioncontrasting

confidence: 61%

“…It has recently been suggested that in adult diabetes there is impaired sensitivity of the beta-receptor of adipocytes, as judged by a reduced output of glycerol from adipose tissue following incubation with adrenaline and phentolamine [5]. Such a defect would be expected to lead to a decreased release of fatty acid from the tissue.…”

mentioning

confidence: 99%

Catecholamine receptor sensitivity and the regulation of lipolysis in adult diabetes

Reckless

Galton

1976

Diabetologia

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Summary. The sensitivity of alpha-and beta-adrenergic receptors, and the antilipolytic actions of prostaglandin E 1 or insulin on adipose tissue of obese diabetic and non-diabetic subjects have been studied. Accumulation of cyclic AMP in adipose tissue and release of glycerol in response to several catecholamines (adrenaline, noradrenaline and isoprenaline) in the presence or absence of an alpha-adrenergic blocker (phentolamine) have been used to assess catecholamine receptor sensitivity. No differences in betareceptor activity were observed between diabetics and non-diabetics, either on glycerol release or accumulation of cyclic AMP; alpha-receptor activity was also similar, except for significantly less accumulation of cyclic AMP in diabetic tissue incubated with noradrenaline and phentolamine (p <0.01). The antilipolytic action of prostaglandin E 1 (at concentrations of 30 fM to 30 pM) on lipolysis (stimulated submaximally with isoprenaline, 10 -7 M) was similar in diabetic and control groups. The antilipolytic action of insulin (from 10 -I~ to 10 -6 M) on lipolysis was also similar between the groups. It is concluded that neither disorders of the catecholamine receptor nor of the antilipolytic actions of prostaglandin E 1 or insulin are responsible for the abnormalities of fatty acid metabolism in adult diabetes.

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“…3). Earlier studies in man have also shown blunting of NA-stimulated lipolysis in adipose tissue of juvenile diabetics [20] and diminished lipolytic response to NA plus phentolamine in adipose tissue of obese maturityonset diabetics [6]. In the present study, the ability of NA to increase the production of cAMP was abolished in adipose tissue of untreated diabetics.…”

Section: Catecholamine-induced Lipolysissupporting

confidence: 70%

Abnormalities in the adrenergic control and the rate of lipolysis in isolated human subcutaneous adipose tissue in diabetes mellitus

Arner¹,

Östman²

1976

Diabetologia

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Subcutaneous adipose tissue was obtained from 9 patients with untreated diabetes mellitus and from 13 obese nondiabetics. After incubation with isoprenaline or noradrenaline, glycerol release and tissue cyclic AMP (cAMP) were determined. Basal glycerol release was twice as rapid from the diabetic adipose tissue. With isoprenaline, the cAMP concentration and the glycerol production was significantly higher in the diabetic adipose tissue. Noradrenaline did not increase glycerol production or cAMP concentration in the diabetic adipose tissue. Subcutaneous adipose tissue was also removed from the diabetics after antidiabetic treatment. Basal lipolysis was significantly reduced and noradrenaline significantly increased both glycerol release and cAMP production. With isoprenaline, cAMP production and glycerol release were significantly less after antidiabetic treatment than in the untreated state. The data provide evidence for increased a-as well as fl-adrenergic receptor sensitivity in human subcutaneous adipose tissue of untreated diabetic patients.

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An abnormality of adrenaline, phentolamine stimulated lipolysis in adipose tissue from obese, maturity-onset diabetics

Cited by 8 publications

References 21 publications

The Effect of Pregnancy on the Control of Lipolysis in Fat Cells Isolated from Human Adipose Tissue

The Effect of Pregnancy on the Control of Lipolysis in Fat Cells Isolated from Human Adipose Tissue

Catecholamine receptor sensitivity and the regulation of lipolysis in adult diabetes

Abnormalities in the adrenergic control and the rate of lipolysis in isolated human subcutaneous adipose tissue in diabetes mellitus

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