An aberrant retinal pathway and visual centers in Xenopus tadpoles share a common cell surface molecule, A5 antigen

Fujisawa, Hajime; Ohtsuki, T.; Takagi, Shin; Tsuji, Toshifumi

doi:10.1016/0012-1606(89)90175-9

Cited by 37 publications

(25 citation statements)

References 22 publications

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“…Previously, we proposed that neuropilin is involved in neuronal cell interaction, and plays a role in axonal growth and fasciculation [33,34] or neuronal recogni tion [30]. The axonal growth-associated expression of neuropilin further supports these hypotheses.…”

Section: Discussionsupporting

confidence: 65%

Growth-Associated Expression of a Membrane Protein, Neuropilin, in <i>Xenopus </i>Optic NerveFibers

1995

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Neuropilin (previously referred to as A5) is a neuronal cell surface protein which is widely distributed in such vertebrate species as Xenopus, chicken and mouse. In these vertebrate species, neuropilin is expressed in particular neuron circuits at particular developmental stages when axonal growth is active. To test whether the expression of neuropilin is associated with axonal growth, we examined expression patterns of neuropilin in developing and regenerating Xenopus optic nerves. In embryos, a neuropilin-specific monoclonal antibody A5 (MAbA5) bound strongly to the optic nerves, and in situ hybridization signals for neuropilin mRNA were prominent in the retinal ganglion cells (RGCs), indicating that developing optic nerve fibers express neuropilin. The binding of MAbA5 to the optic nerve fibers was maximal at stages 41–43, then decreased in the subsequent tadpole life. In tadpoles after stage 50, the binding of MAbA5 to the optic nerves was extremely weak or almost nil, although RGCs expressed considerable amounts of neuropilin mRNA. When the tadpole optic nerves were crushed and prompted to regenerate, however, neuropilin protein reappeared in the optic nerve fibers. The binding of MAbA5 to the regenerating optic nerve fibers was detectable as early as the 5th day and maximal at the 2nd to 3rd weeks after the optic nerve crush, and declined thereafter. These findings suggest that the expression of neuropilin in the optic nerve fibers is regulated in an axonal growth-associated manner.

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Section: Discussionsupporting

confidence: 65%

Growth-Associated Expression of a Membrane Protein, Neuropilin, in <i>Xenopus </i>Optic NerveFibers

1995

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“…The Sema3s are secreted guidance cues that play wide roles during the formation of neuronal circuits [97][98][99][100][101][102]. Sema3s activate holoreceptors composed of at least a ligand binding sub-unit, Neuropilin (Nrp; 1 or 2) [103][104][105][106][107][108][109] and a signaling co-receptor of one of the four Plexin-A (PlexA) family members [102,108,[110][111][112][113][114][115]. In some contexts, recruitment of IgSuperfamily Cell Adhesion Molecules L1-CAMs by neuropilins is also required to trigger Sema3s-mediated down-regulation of adhesive contacts during the repulsive growth cone response [116].…”

Section: Proteolytic Cleavage Of Guidance Receptorsmentioning

confidence: 99%

Axonal commissures in the central nervous system: how to cross the midline?

Nawabi

Castellani

2011

Cell. Mol. Life Sci.

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Organisms with bilateral symmetry elaborate patterns of neuronal projections connecting both sides of the central nervous system at all levels of the neuraxis. During development, these so-called commissural projections navigate across the midline to innervate their contralateral targets. Commissural axon pathfinding has been extensively studied over the past years and turns out to be a highly complex process, implicating modulation of axon responsiveness to the various guidance cues that instruct axon trajectories towards, within and away from the midline. Understanding the molecular mechanisms allowing these switches of response to take place at the appropriate time and place is a major challenge for current research. Recent work characterized several instructive processes controlling the spatial and temporal fine-tuning of the guidance molecular machinery. These findings illustrate the molecular strategies by which commissural axons modulate their sensitivity to guidance cues during midline crossing and show that regulation at both transcriptional and post-transcriptional levels are crucial for commissural axon guidance.

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“…If one assumes that frog and chicken are similar in their expression of N-cadherin, these findings may indicate that N-cadherin serves to guide vertebrate retinal axons along the aberrant pathways by a homophilic adhesion mechanism. This mechanism may work in conjunction with other molecules like A5 (Takagi et al, 1987, 199 l), whose expression is similarly restricted to the developing retinotectal system and the descending trigeminal tract ofthe frog (Fujisawa et al, 1989).…”

Section: Outgrowth Of Sensory Axons On N-cadherin Substratementioning

confidence: 99%

Restricted expression of N- and R-cadherin on neurites of the developing chicken CNS

1992

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The expression of two cadherins, N- and R-cadherin, was mapped in the CNS of chicken embryos of 6–11 d incubation, focusing on the sensory and motor fiber systems. In the spinal cord, the laterally located fibers of the dorsal funiculus express N-cadherin while the medially located fibers do not. These two fiber systems have a different course within the CNS but associate to form the spinal dorsal roots. In the hindbrain, N-cadherin is expressed by the descending trigeminal (general somatic sensory) tract, which is contiguous with the N- cadherin-positive zone of the dorsal funiculus of the spinal cord. R- cadherin is not expressed by sensory fibers, but is expressed by the visceral motor system of the vagus and glossopharyngeal nerves, which are N-cadherin negative. The motor neurites expressing R-cadherin have a different course within the brain than the sensory neurites expressing N-cadherin, although they form the common sensory/motor roots of the vagus nerve at the surface of the brain. The possibility that N-cadherin provides a guidance cue for sensory axon migration within the CNS by a homophilic adhesion mechanism was investigated in vitro. Explants from sensory spinal ganglia expressing N-cadherin were placed on N-cadherin-transfected neuroblastoma cells, and axon outgrowth was visualized. Results showed that the sensory axons defasciculate and closely follow the cell-cell boundaries between transfected cells where high levels of N-cadherin are expressed. These results show that the two cadherins, like members of the immunoglobulin superfamily of molecules, are expressed in a topographically restricted fashion during chick brain development. They furthermore suggest that N- cadherin expression by neurites may play a role in guiding these neurites along CNS paths that express the same molecule.

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An aberrant retinal pathway and visual centers in Xenopus tadpoles share a common cell surface molecule, A5 antigen

Cited by 37 publications

References 22 publications

Growth-Associated Expression of a Membrane Protein, Neuropilin, in <i>Xenopus </i>Optic NerveFibers

Growth-Associated Expression of a Membrane Protein, Neuropilin, in <i>Xenopus </i>Optic NerveFibers

Axonal commissures in the central nervous system: how to cross the midline?

Restricted expression of N- and R-cadherin on neurites of the developing chicken CNS

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