An ABCG-type transporter intensifies ametryn catabolism by Phase III reaction mechanism in rice

Qiao, Yuxin; Zhang, Ai Ping; Li, Ya; Zhang, Nan; Liu, Jintong; Yang, Hong

doi:10.1016/j.jhazmat.2023.131804

Cited by 8 publications

(1 citation statement)

References 54 publications

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“…Phase III metabolism actively removes conjugates and associated catabolic metabolites formed by the phase II metabolism from the cytosol, mainly through adenosine triphosphate (ATP)-dependent transporter proteins, and deposits them into vesicles for temporary storage or further catabolic metabolism [28]; some conjugates are transported to other regions of plant cells, such as aggregation into the cell walls [27]. There are also some reports that classify some of the processes of phase III metabolism as phase IV metabolism, including the re-transport of vesicular products of phase III metabolism back into the cytosol for mineralisation and the incorporation of metabolic intermediates into the conjugated residues, among others [29][30][31][32]. However, at present, the definition of phase IV metabolism is not clear, and opinions are not consistent.…”

Section: Discussionmentioning

confidence: 99%

First Clarification of the Involvement of Glycosyltransferase MdUGT73CG22 in the Detoxification Metabolism of Nicosulfuron in Apple

Zhang,

Zhao,

et al. 2024

Plants

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Nicosulfuron, an acetolactate synthase (ALS) inhibitor herbicide, is a broad-spectrum and highly effective post-emergence herbicide. Glycosyltransferases (GTs) are widely found in organisms and transfer sugar molecules from donors to acceptors to form glycosides or sugar esters, thereby altering the physicochemical properties of the acceptor molecule, such as participating in detoxification. In this study, nine glycosyltransferases in group D of the apple glycosyltransferase family I were predicted to possibly be involved in the detoxification metabolism of ALS-inhibiting herbicides based on gene chip data published online. In order to confirm this, we analysed whether the expression of the nine glycosyltransferase genes in group D was induced by the previously reported ALS-inhibiting herbicides by real-time PCR (polymerase chain reaction). It was found that the ALS-inhibiting herbicide nicosulfuron significantly increased the expression of the MdUGT73CG22 gene in group D. Further investigation of the mechanism of action revealed that the apple glycosyltransferase MdUGT73CG22 glycosylated and modified nicosulfuron both in vivo and ex vivo to form nicosulfuron glycosides, which were involved in detoxification metabolism. In conclusion, a new glycosyltransferase, MdUGT73CG22, was identified for the first time in this study, which can glycosylate modifications of the ALS-inhibiting herbicide nicosulfuron and may be involved in the detoxification process in plants, which can help to further improve the knowledge of the non-targeted mechanism of herbicides.

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