Amylopectin, the Storage Polysaccharide of the Coccidia Eimeria brunetti and E. tenella

Jf, Ryley; Bentley, M.R.; Dj, Manners; Stark,

doi:10.2307/3277227

Cited by 65 publications

(24 citation statements)

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“…The polysaccharide material present in the oocysts of E. brunetti has been isolated and characterised as amylopectin ( 10). The large polysaccharide granules observed, in this study, in developing oocysts, macrogamonts, and merozoites would thus appear to be amylopectin.…”

Section: Discussionmentioning

confidence: 48%

“…In consequence of this, Scholtyseck ( 1 1 ) proposed the term "Coccidienglykogen" for the po!ysaccharide present in the granules of the coccidians. In biochemical studies the polysaccharide of the coccidia was isolated and found to be amylopectin (10). Ryley (9) has reviewed the cytochemical as well as the biochemical aspects of the polysaccharides present in coccidians.…”

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confidence: 99%

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CYTOCHEMICAL ELECTRON MICROSCOPY ON POLYSACCHARIDE GRANULES IN THE ENDOGENOUS FORMS OF EIMERIA BRUNETTI

Dj¹,

Jc³

1977

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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The endogenous stages in the life cycle of Eimeria brunetti were tested for polysaccharide material by electron microscopical cytochemistry using the periodic acid‐thiocarbohydrazideosmium tetroxide (PATO)‐method. The parasites were observed within the epithelial cells of the small intestine of infected domestic fowls. Two types of granules reacted positively for polysaccharide. The first was large, approximately 500 nm by 250 nm. These granules had an appearance similar to the polysaccharide granules reported for other coccidian parasites. They were observed in mature merozoites, macrogamonts, and developing oocysts. The second type of granule was smaller (15–30 nm in diameter) and was only observed at the periphery of the residual cytoplasmic mass of mature microgamonts. It was more similar in appearance to metazoan glycogen than the former. However, since the PATO‐method does not differentiate between different polysaccharides it can not be proven if these granules in fast contain glycogen. The WFB I of the macrogamete and the outer layer of the oocyst wall gave a slight positive reaction but after examination of the controls it appeared that this was not a specific reaction for polysaccharides.

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Section: Discussionmentioning

confidence: 48%

mentioning

confidence: 99%

CYTOCHEMICAL ELECTRON MICROSCOPY ON POLYSACCHARIDE GRANULES IN THE ENDOGENOUS FORMS OF EIMERIA BRUNETTI

Dj¹,

Jc³

1977

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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“…These discrepancies can almost certainly be ascribed to contamination of the walls with the internal contents of the oocyst, as the TEM images presented here document the presence of large numbers of amylopectin granules in the samples prepared following the method described by Stotish et al (1978). The high levels of glucose reported by Stotish et al (1978) -much higher than detected by the GC-MS analyses reported here -support this contention as amylopectin is a polymer of glucose monomers joined by alpha-1,4 linkages (Ryley et al 1969(Ryley et al , 1973.…”

Section: Biochemical Composition Of the Oocyst Wallmentioning

confidence: 45%

Oocyst wall formation and composition in coccidian parasites

Mai¹,

Sharman²,

Walker³

et al. 2009

Mem. Inst. Oswaldo Cruz

121

106

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“…beauchampi is the paraglycogen granules whose amylopectin nature is well known from the works of Ryley et al (1969) and Shrevel (1970); however, the elaboration process is still not fully understood.…”

Section: Discussionmentioning

confidence: 99%

Fine structure of merozoites ofEimeria ? beauchampiLeger and Duboscq, 1917 inGlossobalanus minutus(Kow.) (Enteropneusta)

Fernández

Pardos

Benito

1989

Ann. Parasitol. Hum. Comp.

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SUMMARY. Merozoites of Eimeria? beauchampi Leger and Duboscq, 1917 from the digestive epithelium of the hepatic region of the enteropneust Glossobalanus minutus (Kow.) have been studied by electron microscopy lor the first time. The merozoites show typical coccidian features with a trilaminate pellicle, an apical polar ring, a conoid with two accessory rings, micronemes, and two rhoptries whose ductules are directed along a central intra-conoidal microtubule, above which a spherical vesicle occurs. Approximately 50 subpellicular microtubules run from the apical end to the nuclear region of the parasites. Lipid droplets, amylopectin granules and a large mito chondrion also occur in the cytoplasm. The presence of a refractile-like body is a remarquable feature of the merozoites studied. The present data are discussed in the light of the available literature. Key

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Amylopectin, the Storage Polysaccharide of the Coccidia Eimeria brunetti and E. tenella

Cited by 65 publications

References 0 publications

CYTOCHEMICAL ELECTRON MICROSCOPY ON POLYSACCHARIDE GRANULES IN THE ENDOGENOUS FORMS OF EIMERIA BRUNETTI

CYTOCHEMICAL ELECTRON MICROSCOPY ON POLYSACCHARIDE GRANULES IN THE ENDOGENOUS FORMS OF EIMERIA BRUNETTI

Oocyst wall formation and composition in coccidian parasites

Fine structure of merozoites ofEimeria ? beauchampiLeger and Duboscq, 1917 inGlossobalanus minutus(Kow.) (Enteropneusta)

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