Amyloidogenic behavior of different intermediate state of stem bromelain: A biophysical insight

Zaman, Masihuz; Ehtram, Aquib; Chaturvedi, Sumit Kumar; Nusrat, Saima; Khan, Rizwan Hasan

doi:10.1016/j.ijbiomac.2016.05.107

Cited by 28 publications

(10 citation statements)

References 44 publications

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“…Thus, secondary structure alterations in the absence and presence of clofazimine were observed. As shown in Figure , 2 negative minima in far UV range of 208 and 222 nm clearly indicate the α‐helix nature of protein as shown by others . The CD spectrum of native protein, ie, BLC, shows 2 characteristic peaks at or around 222 (due to n → pi* transition) and 208 nm (due to pi → pi* transition), signifying the presence of α‐helical structure of BLC as observed by Abdelhameed et al The binding of clofazimine with BLC leads to an enhancement in the negative minima at 208 and 222 nm, indicating the induction of α‐helix.…”

Section: Resultssupporting

confidence: 51%

Elucidating the interaction of clofazimine with bovine liver catalase; a comprehensive spectroscopic and molecular docking approach

Zaman

Nusrat

Zakariya

et al. 2017

J of Molecular Recognition

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Nowadays, understanding of interface between protein and drugs has become an active research area of interest. These types of interactions provide structural guidelines in drug design with greater clinical efficacy. Thus, structural changes in catalase induced by clofazimine were monitored by various biophysical techniques including UV-visible spectrometer, fluorescence spectroscopy, circular dichroism, and dynamic light scattering techniques. Increase in absorption spectra (UV-visible spectrum) confers the complex formation between drug and protein. Fluorescence quenching with a binding constants of 2.47 × 10 M revealed that clofazimine binds with protein. Using fluorescence resonance energy transfer, the distance (r) between the protein (donor) and drug (acceptor) was found to be 2.89 nm. Negative Gibbs free energy change (ΔG°) revealed that binding process is spontaneous. In addition, an increase in α-helicity was observed by far-UV circular dichroism spectra by adding clofazimine to protein. Dynamic light scattering results indicate that topology of bovine liver catalase was slightly altered in the presence of clofazimine. Hydrophobic interactions are the main forces between clofazimine and catalase interaction as depicted by molecular docking studies. Apart from hydrophobic interactions, some hydrogen bonding was also observed during docking method. The results obtained from the present study may establish abundant in optimizing the properties of ligand-protein mixtures relevant for numerous formulations.

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Section: Resultssupporting

confidence: 51%

Elucidating the interaction of clofazimine with bovine liver catalase; a comprehensive spectroscopic and molecular docking approach

Zaman

Nusrat

Zakariya

et al. 2017

J of Molecular Recognition

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“…In the last decade, nanoparticles (NPs) have been deployed in the field of biomedical sciences for targeted delivery of drug, imaging, diagnosis and treatment of diseases and development of antimicrobials (Ramar et al, 2015). Metal and metal-oxide nanoparticles demonstrate novel physico-chemical properties that are not associated with individual or bulk molecules (Daniel and Astruc, 2004, Zaman et al, 2016, Zaman et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Green synthesis and structural classification of Acacia nilotica mediated-silver doped titanium oxide (Ag/TiO2) spherical nanoparticles: Assessment of its antimicrobial and anticancer activity

Rao

Riyazuddin

Babji

et al. 2019

Saudi Journal of Biological Sciences

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Current exanimation reports, green fabrication of silver doped TiO2 nanoparticles (Ag/TiO2) using aqueous extract of Acacia nilotica as bio-reductant and assess its potential as antimicrobial and anticancer agent. The obtained spherical Ag/TiO2 were characterized by various analytical techniques including FTIR, (XRD), (FE-SEM EDS), and (TEM). Synthesized Ag/TiO2 demonstrated broad spectrum antibacterial and anticandidal activity. The order of antimicrobial activity was found to be E. coli > C. albicans > MRSA > P. aeruginosa. In addition, cytotoxicity and oxidative stress of Ag/TiO2 nanoparticles in (MCF-7) cells was also investigated. Outcomes of MTT assay showed concentration dependent reduction in cell viability. Further, synthesized NPs reduced the level of glutathione, induced ROS generation and lipid peroxidation in the treated cells. Therefore, it is envisaged that these spherical nanoparticles may be exploited in drug delivery, pharmaceutical, and food industry.

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“…After 2 min, excess fluid was removed and samples containing copper grids were negative stained with 2% (w/v) aqueous uranyl acetate solution. Uranyl acetate is known to produce high electron density, image contrast, and impart fine grained impression to the image …”

Section: Methodsmentioning

confidence: 99%

“…Uranyl acetate is known to produce high electron density, image contrast, and impart fine grained impression to the image. 46,47 3.10 | Cell culture…”

Section: Transmission Electron Microscopy Measurementsmentioning

confidence: 99%

Amino group of salicylic acid exhibits enhanced inhibitory potential against insulin amyloid fibrillation with protective aptitude toward amyloid induced cytotoxicity

Zaman

Khan

Zakariya

et al. 2018

J of Cellular Biochemistry

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Protein misfolding and aggregation lead to amyloid generation that in turn may induce cell membrane disruption and leads to cell apoptosis. In an effort to prevent or treat amyloidogenesis, large number of studies has been paying attention on breakthrough of amyloid inhibitors. In the present work, we aim to access the effect of two drugs, that is, acetylsalicylic acid and 5-amino salicylic acid on insulin amyloids by using various biophysical, imaging, cell viability assay, and computational approaches. We established that both drugs reduce the turbidity, light scattering and fluorescence intensity of amyloid indicator dye thioflavin T. Premixing of drugs with insulin inhibited the nucleation phase and inhibitory potential was boosted by increasing the concentration of the drug. Moreover, addition of drugs at the studied concentrations attenuated the insulin fibril induced cytotoxicity in breast cancer cell line MDA-MB-231. Our results highlight the amino group of salicylic acid exhibited enhanced inhibitory effects on insulin fibrillation in comparison to acetyl group. It may be due to presence of amino group that helps it to prolong the nucleation phase with strong binding as well as disruption of aromatic and hydrophobic stacking that plays a key role in amyloid progression.

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Amyloidogenic behavior of different intermediate state of stem bromelain: A biophysical insight

Cited by 28 publications

References 44 publications

Elucidating the interaction of clofazimine with bovine liver catalase; a comprehensive spectroscopic and molecular docking approach

Elucidating the interaction of clofazimine with bovine liver catalase; a comprehensive spectroscopic and molecular docking approach

Green synthesis and structural classification of Acacia nilotica mediated-silver doped titanium oxide (Ag/TiO2) spherical nanoparticles: Assessment of its antimicrobial and anticancer activity

Amino group of salicylic acid exhibits enhanced inhibitory potential against insulin amyloid fibrillation with protective aptitude toward amyloid induced cytotoxicity

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