Amyloid precursor protein gene isoforms in Alzheimer’s disease and other neurodegenerative disorders

Panegyres, Peter K.; Zafiris-Toufexis, K.; Kakulas, B. A.

doi:10.1016/s0022-510x(99)00311-1

Cited by 18 publications

(16 citation statements)

References 48 publications

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“…The OD has previously been used for determination of the regional distribution of WMD in AD as well as estimation of glial numbers in the department (Sjobeck et al, 2005, in press; Sjobeck and Englund, 2003;). Furthermore, others used OD for quantification of gene expression using in situ hybridization and in immunohistochemical analysis (Panegyres et al, 2000;Hellier et al, 2002;Wang and Li, 2003).…”

Section: Assessment Of Severity Of White Matter Disease and Myelin Dementioning

confidence: 99%

Decreasing myelin density reflected increasing white matter pathology in Alzheimer's disease—a neuropathological study

Sjöbeck

Haglund

Englund

2005

Int. J. Geriat. Psychiatry

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Section: Assessment Of Severity Of White Matter Disease and Myelin Dementioning

confidence: 99%

Decreasing myelin density reflected increasing white matter pathology in Alzheimer's disease—a neuropathological study

Sjöbeck

Haglund

Englund

2005

Int. J. Geriat. Psychiatry

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“…390). A statewide mailout to general practitioners, neurologists, psychiatrists, geriatricians and nursing homes was performed (Panegyres et al, 2000).…”

Section: Patient Recruitmentmentioning

confidence: 99%

The mRNA of the NR1 subtype of glutamate receptor in Alzheimer's disease

Panegyres

Zafiris-Toufexis

Kakulas

2002

Journal of Neural Transmission

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Glutamate has been implicated in the pathogenesis of Alzheimer's disease (AD). Controversial data exists regarding changes in the N-methyl-D-aspartate (NMDA) receptor complex in AD. We wished to elucidate the hypothesis that the NMDA receptor system is involved in the pathogenesis of AD using a gene expression approach targeting the mRNA of the universal subtype of the NMDA receptor NR1. This was performed using in situ hybridization and antisense 35S-labelled oligonucleotides on brain tissue collected at post-mortem. Relative mRNA expression was measured in standardised optical density units (OD units) using videodensitometry without knowledge of the diagnosis. The study population consisted of AD (n = 6) and neurodegenerative non-Alzheimer controls (non-AD, n = 14). Gene expression was measured in the frontal lobe, superior temporal gyrus and three areas within the hippocampus. We have observed no significant differences in the relative mRNA expression of the NR1 subtype of glutamate receptor in the following regions: frontal lobe AD = 60.7 +/- 14.1 OD units mRNA (x +/- 1SE) vs 52.6 +/- 1 in non-AD (Mann-Whitney test, p = 0.477); the superior temporal gyrus: AD = 53.3 +/- 13.9 vs 38.2 +/- 7 (p = 0.37); the CA1 region: AD = 37.8 +/- 7.75 vs 81.5 +/- 25.7 (p = 0.66); subiculum AD = 46.7 +/- 11.0 vs 105 +/- 43.3 (p = 0.82); parahippocampal gyrus AD = 36.6 +/- 9.3 vs 81.7 +/- 40.6 (p = 0.90). There were trends to a reduction in NR1 mRNA in the hippocampus and increased NR1 within the frontal and superficial temporal gyrus which were not significant. There was variation within and between all patients with and without AD in the magnitude of NR1 expression in all anatomical regions studied. The findings suggest heterogeneity in the involvement of the post-synaptic glutamatergic system in AD.

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“…nuclease protection assays versus in situ hybridization). A relatively recent study using radioactive in situ hybridization analysis of APP isoforms and larger numbers of AD patients and neurodegenerative controls has revealed no differences in differential mRNA expression of APP isoforms [340]. An investigation using PT-PCR in AD post-mortem brains showed an increase in the KPI+ isoforms controlling for age at death, brain pH and gender [341].…”

Section: Human Studiesmentioning

confidence: 97%

The Functions of the Amyloid Precursor Protein Gene and Its Derivative Peptides: II Experimental Evidence and Clinical Studies

Panegyres¹,

Atkins²

2011

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Amyloid precursor protein gene isoforms in Alzheimer’s disease and other neurodegenerative disorders

Cited by 18 publications

References 48 publications

Decreasing myelin density reflected increasing white matter pathology in Alzheimer's disease—a neuropathological study

Decreasing myelin density reflected increasing white matter pathology in Alzheimer's disease—a neuropathological study

The mRNA of the NR1 subtype of glutamate receptor in Alzheimer's disease

The Functions of the Amyloid Precursor Protein Gene and Its Derivative Peptides: II Experimental Evidence and Clinical Studies

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