Amyloid-like aggregates of neuronal tau induced by formaldehyde promote apoptosis of neuronal cells

Nie, Chun Lai; Wang, Xing Sheng; Liu, Ying; Perrett, Sarah; He, Rong

doi:10.1186/1471-2202-8-9

Cited by 68 publications

(33 citation statements)

References 61 publications

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“…Our previous results showed that extracellular tau aggregates were toxic to neuronal cells [27]. We detected intracellular tau aggregation by formaldehyde in cell apoptosis.…”

Section: Resultsmentioning

confidence: 63%

“…Tau-40 contains 44 lysine and 2 cysteine residues (NP_005901) [1], [30], which should react with formaldehyde. 5,5′-Dithio-bis(2-nitrobenzoic acid) (DTNB) modification was used to check the thiol groups of tau incubated under different concentrations of formaldehyde (Figure 6) whilst o -phthaldehyde (OPT) modification was used to detect the amino groups of tau under the same conditions as described previously [27]. Both DTNB absorbance (412 nm) and OPT fluorescence (E m 455 nm/E x 340 nm) decreased following the increase of formaldehyde concentration.…”

Section: Resultsmentioning

confidence: 99%

“…According to a recent report, humans are uniquely sensitive to the toxicity of methanol, as they have limited capacity to oxidize and detoxify formic acid. Thus, the toxicity of methanol in humans is characterized by formic acidaemia, metabolic acidosis, blindness or serious visual impairment, mild central nervous system depression and even death [23], [27], [28]. However, methanol toxicosis induces progressive complications to CNS.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, we investigated the toxicity of formaldehyde-induced tau aggregates on human neuroblastoma cells (SH-SY5Y cell line) and rat hippocampal cells [27]. The results showed that low concentrations (0.01–0.1%) of formaldehyde are sufficient to induce formation of amyloid-like tau aggregates, which can induce apoptosis of both SH-SY5Y and hippocampal cells.…”

Section: Introductionmentioning

confidence: 99%

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Formaldehyde at Low Concentration Induces Protein Tau into Globular Amyloid-Like Aggregates In Vitro and In Vivo

et al. 2007

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Recent studies have shown that neurodegeneration is closely related to misfolding and aggregation of neuronal tau. Our previous results show that neuronal tau aggregates in formaldehyde solution and that aggregated tau induces apoptosis of SH-SY5Y and hippocampal cells. In the present study, based on atomic force microscopy (AFM) observation, we have found that formaldehyde at low concentrations induces tau polymerization whilst acetaldehyde does not. Neuronal tau misfolds and aggregates into globular-like polymers in 0.01–0.1% formaldehyde solutions. Apart from globular-like aggregation, no fibril-like polymerization was observed when the protein was incubated with formaldehyde for 15 days. SDS-PAGE results also exhibit tau polymerizing in the presence of formaldehyde. Under the same experimental conditions, polymerization of bovine serum albumin (BSA) or α-synuclein was not markedly detected. Kinetic study shows that tau significantly misfolds and polymerizes in 60 minutes in 0.1% formaldehyde solution. However, presence of 10% methanol prevents protein tau from polymerization. This suggests that formaldehyde polymerization is involved in tau aggregation. Such aggregation process is probably linked to the tau's special “worm-like” structure, which leaves the ε-amino groups of Lys and thiol groups of Cys exposed to the exterior. Such a structure can easily bond to formaldehyde molecules in vitro and in vivo. Polymerizing of formaldehyde itself results in aggregation of protein tau. Immunocytochemistry and thioflavin S staining of both endogenous and exogenous tau in the presence of formaldehyde at low concentrations in the cell culture have shown that formaldehyde can induce tau into amyloid-like aggregates in vivo during apoptosis. The significant protein tau aggregation induced by formaldehyde and the severe toxicity of the aggregated tau to neural cells may suggest that toxicity of methanol and formaldehyde ingestion is related to tau misfolding and aggregation.

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“…Our previous results showed that extracellular tau aggregates were toxic to neuronal cells [27]. We detected intracellular tau aggregation by formaldehyde in cell apoptosis.…”

Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Formaldehyde at Low Concentration Induces Protein Tau into Globular Amyloid-Like Aggregates In Vitro and In Vivo

et al. 2007

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“…This assay (Rojas Quijano et al, 2006) was performed by adding a freshly-prepared stock solution of Congo Red in 100 mmol/L potassium phosphate (pH 7.2) to RCC-fibrinogen adducts (3 mg/mL) to give a final Congo Red concentration of 5 μmol/L as described previously (Nie et al, 2007).…”

Section: Congo Red Binding Assaymentioning

confidence: 99%

Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen

Qiang

Zhang

et al. 2012

Protein Cell

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Current state of research on non‐human primate models of Alzheimer’s disease

Zhang

Qin

2019

Anim Models and Exp Med

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With the increasingly serious aging of the global population, dementia has already become a severe clinical challenge on a global scale. Dementia caused by Alzheimer's disease (AD) is the most common form of dementia observed in the elderly, but its pathogenetic mechanism has still not been fully elucidated. Furthermore, no effective treatment strategy has been developed to date, despite considerable efforts. This can be mainly attributed to the paucity of animal models of AD that are sufficiently similar to humans. Among the presently established animal models, non‐human primates share the closest relationship with humans, and their neural anatomy and neurobiology share highly similar characteristics with those of humans. Thus, there is no doubt that these play an irreplaceable role in AD research. Considering this, the present literature on non‐human primate models of AD was reviewed to provide a theoretical basis for future research.

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Amyloid-like aggregates of neuronal tau induced by formaldehyde promote apoptosis of neuronal cells

Cited by 68 publications

References 61 publications

Formaldehyde at Low Concentration Induces Protein Tau into Globular Amyloid-Like Aggregates In Vitro and In Vivo

Formaldehyde at Low Concentration Induces Protein Tau into Globular Amyloid-Like Aggregates In Vitro and In Vivo

Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen

Current state of research on non‐human primate models of Alzheimer’s disease

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