“…We observe bands consistent with monomeric TMEM106B(120-254), molecular weight of 15.49 kDa, at $16 kDa (Figure S6) and multiple bands $25 kDa (Figure S6) that may correspond to glycosylated TMEM106B(120-254) monomers. Notably, there are many higher molecular weight bands at 110 kDa, 135 kDa, and 190 kDa that are consistent with TMEM106B oligomerization (Jiang et al, 2022) (Figure S6). The general trend we observe is that these high molecular weight species are more abundant in pathological cases compared to non-pathological controls, although there are exceptions (e.g., FTLD-TDP case 4 is a sporadic case that shows a high concentration of TMEM106B fibrils by cryo-EM but not by western blot).…”