Amyloid Fibrils Enhance Transport of Metal Nanoparticles in Living Cells and Induced Cytotoxicity

Bolisetty, Sreenath; Boddupalli, Chandra Sekhar; Handschin, Stephan; Chaitanya, Krishna; Adamčík, Jozef; Saito, Yasuyuki; Manz, Markus G.; Mezzenga, Raffaele

doi:10.1021/bm500647n

Cited by 50 publications

(48 citation statements)

References 40 publications

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“…Shortly after this work, another amyloid system based on the non‐toxic β‐lactoglobulin, was shown to enhance transport of metal nanoparticles in living cells . The authors used two types of cells, a cell line and dendritic cells, to demonstrate that the same gold nanoparticles penetrate with a three‐fold stronger efficiency when conjugated to amyloid fibrils compared to bare nanoparticles.…”

Section: Functional Amyloid In Technological Applicationsmentioning

confidence: 99%

Amyloid Fibrils as Building Blocks for Natural and Artificial Functional Materials

2016

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Proteinaceous materials based on the amyloid core structure have recently been discovered at the origin of biological functionality in a remarkably diverse set of roles, and attention is increasingly turning towards such structures as the basis of artificial self-assembling materials. These roles contrast markedly with the original picture of amyloid fibrils as inherently pathological structures. Here we outline the salient features of this class of functional materials, both in the context of the functional roles that have been revealed for amyloid fibrils in nature, as well as in relation to their potential as artificial materials. We discuss how amyloid materials exemplify the emergence of function from protein self-assembly at multiple length scales. We focus on the connections between mesoscale structure and material function, and demonstrate how the natural examples of functional amyloids illuminate the potential applications for future artificial protein based materials.

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Section: Functional Amyloid In Technological Applicationsmentioning

confidence: 99%

Amyloid Fibrils as Building Blocks for Natural and Artificial Functional Materials

2016

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“…In contrast, β-lactoglobulin (bLg) is a natural whey protein which hydrolyzes into small peptide fragments upon heating and acid exposure (pH 2), and subsequently self-assembles into functional bLg amyloid fibrils. 7, 8 Efficient in vitro iron delivery and wastewater purification have been recently demonstrated using bLg amyloids, 9, 10 pointing to the untapped potential of this biomaterial.…”

Section: Introductionmentioning

confidence: 99%

Cofibrillization of Pathogenic and Functional Amyloid Proteins with Gold Nanoparticles against Amyloidogenesis

et al. 2017

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Biomimetic nanocomposites and scaffolds hold the key to a wide range of biomedical applications. Here we show, for the first time, a facile scheme of co-fibrillizing pathogenic and functional amyloid fibrils via gold nanoparticles (AuNPs) and their applications against amyloidogenesis. This scheme was realized by β-sheet stacking between human islet amyloid polypeptide (IAPP) and the β-lactoglobulin ‘corona’ of the AuNPs, as revealed by transmission electron microscopy, 3D atomic force microscopy, circular dichroism spectroscopy and molecular dynamics simulations. The biomimetic AuNPs eliminated IAPP toxicity, enabled X-ray destruction of IAPP amyloids, and allowed dark-field imaging of pathogenic amyloids and their immunogenic response by human T cells. In addition to providing a viable new nanotechnology against amyloidogenesis, this study has implications for understanding the in vivo cross-talk between amyloid proteins of different pathologies.

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“…10 For instance, Bolisetty et al generated amyloid fibrils from b-lactoglobulin, and found that their conjugation to metal nanoparticles enhanced cell permeability and toxicity. 11 Haratifar et al used tea catechin-casein complex to significantly decrease cancer cell proliferation. 12 However, these approaches were not subjected to in vivo experiments.…”

Section: Introductionmentioning

confidence: 99%

Milk derived colloid as a novel drug delivery carrier for breast cancer

Hayashi

Silanikove

Chang

et al. 2015

Cancer Biology & Therapy

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Triple negative breast cancer has an extremely poor prognosis when chemotherapy is no longer effective. To overcome drug resistance, novel drug delivery systems based on nanoparticles have had remarkable success. We produced a novel nanoparticle component 'MDC' from milk-derived colloid. In order to evaluate the anti-cancer effect of MDC, we conducted in vitro and in vivo experiments on cancer cell lines and a primary tumor derived breast xenograft. Doxorubicin (Dox) conjugated to MDC (MDC-Dox) showed higher cancer cell growth inhibition than MDC alone especially in cell lines with high EGFR expression. In a mouse melanoma model, MDC-Dox significantly suppressed tumor growth when compared with free Dox. Moreover, in a primary tumor derived breast xenograft, one of the mice treated with MDC-Dox showed partial regression, while mice treated with free Dox failed to show any suppression of tumor growth. We have shown that a novel nanoparticle compound made of simple milk-derived colloid has the capability for drug conjugation, and serves as a tumor-specific carrier of anti-cancer drugs. Further research on its safety and ability to carry various anti-cancer drugs into multiple drug-resistant primary breast models is warranted.

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Amyloid Fibrils Enhance Transport of Metal Nanoparticles in Living Cells and Induced Cytotoxicity

Cited by 50 publications

References 40 publications

Amyloid Fibrils as Building Blocks for Natural and Artificial Functional Materials

Amyloid Fibrils as Building Blocks for Natural and Artificial Functional Materials

Cofibrillization of Pathogenic and Functional Amyloid Proteins with Gold Nanoparticles against Amyloidogenesis

Milk derived colloid as a novel drug delivery carrier for breast cancer

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