Amyloid Beta and Tau as Alzheimer’s Disease Blood Biomarkers: Promise From New Technologies

Lue, Lih‐Fen; Guerra, André; Walker, Douglas G.

doi:10.1007/s40120-017-0074-8

Cited by 97 publications

(58 citation statements)

References 78 publications

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“…immunoaffinity‐based assays) now available in the market such as immunomagnetic reduction (IMR) and single molecule array (SIMOA) must be considered in future studies to overcome the challenges of detection encountered using ELISA‐based technique. Both IMR and SIMOA, although promising, still need validation in different populations by means of multicentre studies (Lue, Guerra, & Walker, ). Lastly, readers should interpret the present results with caution.…”

Section: Discussionmentioning

confidence: 99%

Periodontitis and systemic markers of neurodegeneration: A case–control study

Leira

Carballo

Orlandi

et al. 2020

J Clinic Periodontology

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Aim:To investigate whether periodontitis is associated with amyloid beta (Aβ) peptides and whether systemic inflammation could act as a potential mediator of this link. Materials and Methods:A case-control study was designed including 75 patients with periodontitis (cases) and 75 age-balanced and gender-matched participants without periodontitis (controls). Full-mouth periodontal evaluation was performed in all participants. Demographic, clinical and behaviour data were also recorded. Fasting blood samples were collected, and serum levels of interleukin 6 (IL-6), high-sensitivity C-reactive protein (hs-CRP), Aβ 1-40 and Aβ 1-42 were determined.Results: Cases showed higher levels of IL-6 (8.7 ± 3.2 vs. 4.8 ± 0.5 pg/ml), hs-CRP (3.3 ± 1.2 vs. 0.9 ± 0.7 mg/L), Aβ 1-40 (37.3 ± 6.0 vs. 30.3 ± 1.8 pg/ml) and Aβ 1-42 (54.5 ± 10.6 vs. 36.5 ± 10.0 pg/ml) when compared to controls (all p < .001). Diagnosis of periodontitis was statistically significantly associated with cir-culating Aβ 1-40 ( coefficient adjusted = 6.9, 95% CI: 5.4-8.3; p < .001) and Aβ ( coefficient adjusted = 17.8, 95% CI: 14.4-21.3; p < .001). Mediation analysis confirmed hs-CRP and IL-6 as mediators of this association. Conclusions:Periodontitis is associated with increased peripheral levels of Aβ. This finding could be explained by enhanced systemic inflammation that can be seen in patients with periodontitis.

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Section: Discussionmentioning

confidence: 99%

Periodontitis and systemic markers of neurodegeneration: A case–control study

Leira

Carballo

Orlandi

et al. 2020

J Clinic Periodontology

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“…Over the years, much effort has been devoted to global research and as a result several blood-based biomarkers have been developed [19,20]. These blood-based biomarkers have demonstrated high correlation between the level of Aβ42, Aβ40, and the risk of AD [21,22], but they require highly sensitive equipment to detect low-concentrated target proteins. On the other hand, MDS-OAβ utilizes spiking of purified synthetic Aβ into plasma and incubation thereafter to measure the level of AβO tendencies in plasma [12].…”

Section: Discussionmentioning

confidence: 99%

Association of Plasma Oligomerized Beta Amyloid with Neurocognitive Battery Using Korean Version of Consortium to Establish a Registry for Alzheimer’s Disease in Health Screening Population

et al. 2020

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The increasing prevalence of Alzheimer’s disease (AD) has become a global phenomenon presenting serious social and health challenges. For detecting early molecular changes in the disease, several techniques to measure varied species of amyloid beta in the peripheral blood have been recently developed, but the efforts to associate them with cognitive assessments have yet to produce sufficient data. We prospectively collected participants from the consecutive population who visited our center for brain health screening. In total, 97 participants (F:M = 58:39) aged 69.4 ± 7.52 were assessed. Participants performed the Korean version of the Consortium to Establish a Registry for Alzheimer’s disease (CERAD-K), the clinical dementia rating (CDR), plasma oligomeric amyloid-β (OAβ) level tests, routine blood tests, ApoE genotype, and brain MRI. Among total population, 55.7% had a CDR of 0, and 40.2% had a CDR of 0.5. The results showed that word memory and word recall, and the total scores of the CERAD-K were negatively correlated with the plasma OAβ level. With a cut-off value of 0.78 ng/mL for the OAβ level and a −1.5 standard deviation of age/sex/education adjusted norms for the CERAD-K; naming, word memory, word recall, word recognition, and total score were significantly correlated with the OAβ level. No correlation between the OAβ level and mini-mental status examination was found. Our results demonstrate that the level of plasma OAβ was well correlated with the measure of cognitive function through the CERAD-K in the field data collected from consecutive populations. Studies on longitudinal comparisons with large cohorts will further validate the diagnostic value of plasma OAβ as a useful biomarker for screening AD and predicting progression.

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“…Recently, new ultrasensitive technologies with superior sensitivity and specificity for measuring blood-base biomarkers, such as an immunomagnetic reduction (IMR) assay, single molecule array (SIMOA), immunocapture-based multiplexing systems (xMAP) immunoprecipitation-mass spectrometry (IP-MS), and modified sandwich ELISA, have been developed [9][10][11][12]. Clinical studies using these technologies reported that significant changes in concentrations of plasma Aβ 1-42 and the ratio of plasma Aβ 1-42 /Aβ 1-40 are associated with risk of AD [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Stability of Plasma Amyloid-β 1–40, Amyloid-β 1–42, and Total Tau Protein over Repeated Freeze/Thaw Cycles

Liu¹,

Chiu

Lin

et al. 2020

Dement Geriatr Cogn Disord Extra

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Introduction: Blood biomarkers of Alzheimer's disease (AD) have attracted much attention of researchers in recent years. In clinical studies, repeated freeze/thaw cycles often occur and may influence the stability of biomarkers. This study aims to investigate the stability of amyloid-β 1-40 (Aβ 1-40 ), amyloid-β 1-42 (Aβ 1-42 ), and total tau protein (T-tau) in plasma over freeze/thaw cycles. Methods: Plasma samples from healthy controls (n = 2), AD patients (AD, n = 3) and Parkinson's disease patients (PD, n = 3) were collected by standardized procedure and immediately frozen at -80 ° C. Samples underwent 5 freeze/thaw (-80 ° C/room temperature) cycles. The concentrations of Aβ 1-40 , Aβ 1-42 , and T-tau were monitored during the freeze/ thaw tests using an immunomagnetic reduction (IMR) assay. The relative percentage of concentrations after every freeze/thaw cycle was calculated for each biomarker. Results: A tendency of decrease in the averaged relative percentages over samples through the freeze and thaw cycles for Aβ 1-40 (100 to 97.11%), Aβ 1-42 (100 to 94.99%), and T-tau (100 to 95.65%) was found. However, the decreases were less than 6%. For all three biomarkers, no statistical significance was found between the levels of fresh plasma and those of the plasma experiencing 5 freeze/thaw cycles (p > 0.1). Conclusions: Plasma Aβ 1-40 , Aβ 1-42 , and T-tau are stable through 5 freeze/thaw cycles measured with IMR.

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Amyloid Beta and Tau as Alzheimer’s Disease Blood Biomarkers: Promise From New Technologies

Cited by 97 publications

References 78 publications

Periodontitis and systemic markers of neurodegeneration: A case–control study

Periodontitis and systemic markers of neurodegeneration: A case–control study

Association of Plasma Oligomerized Beta Amyloid with Neurocognitive Battery Using Korean Version of Consortium to Establish a Registry for Alzheimer’s Disease in Health Screening Population

Stability of Plasma Amyloid-β 1–40, Amyloid-β 1–42, and Total Tau Protein over Repeated Freeze/Thaw Cycles

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