Amylase Production from Thermophilic Bacillus sp. BCC 021-50 Isolated from a Marine Environment

Abstract

Abstract:The high cost of fermentation media is one of the technical barriers in amylase production from microbial sources. Amylase is used in several industrial processes or industries, for example, in the food industry, the saccharification of starchy materials, and in the detergent and textile industry. In this study, marine microorganisms were isolated to identify unique amylase-producing microbes in starch agar medium. More than 50 bacterial strains with positive amylase activity, isolated from marine water and soil, were screened for amylase production in starch agar medium. Bacillus sp. BCC 021-50 was found to be the best amylase-producing strain in starch agar medium and under submerged fermentation conditions. Next, fermentation conditions were optimized for bacterial growth and enzyme production. The highest amylase concentration of 5211 U/mL was obtained after 36 h of incubation at 50 • C, pH 8.0, using 20 g/L molasses as an energy source and 10 g/L peptone as a nitrogen source. From an application perspective, crude amylase was characterized in terms of temperature and pH. Maximum amylase activity was noted at 70 • C and pH 7.50. However, our results show clear advantages for enzyme stability in alkaline pH, high-temperature, and stability in the presence of surfactant, oxidizing, and bleaching agents. This research contributes towards the development of an economical amylase production process using agro-industrial residues.

Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

“…The culture was incubated at 37˚C on a rotary shaker at 150 rpm and thereafter sampled (2 mL) every 2 h. At the time of sampling, absorbance reading at 600 nm was recorded using a UV/VIS spectrophotometer. In another experiment, bacteria were screened for a series of biochemical reaction tests: amylase [28]; [29]; protease [30]; lipase [31]; catalase; urease; acetate utilization. A light microscope (Olympus CH, Japan) was used to observe morphological characteristics and Gram's reaction of the bacterial strains.…”

Separación de tinta de papel impreso por actividad amilolítica microbiana

“…El valor óptimo de 70 ºC encontrado para la actividad amilolítica en este trabajo es inferior al de 95 ºC de la amilasa de Thermus filiformis (Egas et al, 1998), similar al de 70 ºC de la amilasa de Bacillus sp. termófilo marino BCC 021-50 (Ahmed et al, 2017) pero superior al de 60 ºC de la amilasa de Thermus sp. ( Fatoni y Zusfahair, 2012).…”

“…El valor de pH 6 encontrado como valor óptimo para la actividad amilolítica es inferior al de 7.5 de la amilasa de Bacillus sp. BCC021-50 (Ahmed et al, 2017), al de 7.0 de la α-amilasa de Geobacillus stearothermophilus (Garrett y Grisham, 1999) pero similar al de 5.5-6.5 de la amilasa de Thermus sp. (Shaw et al, 1995) y al de 5.5-6.0 de la amilasa de Thermus filiformis (Egas et al, 1998).…”

Seed biopriming with P- and K-solubilizingEnterobacter hormaecheisp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

“…The culture was incubated at 37°C on a rotary shaker at 150 rpm and thereafter sampled (2 mL) every 2 h. At the time of sampling, absorbance reading at 600 nm was recorded using UV/VIS spectrophotometer. In another experiment, bacteria were screened for a series of biochemical reaction tests: amylase [27]; [28]; protease [29]; lipase [30]; catalase; urease; acetate utilization. Olympus CH light microscope (Tokyo, Japan) was used to observe morphological characteristics and Gram’s reaction of the bacterial isolates.…”