Amygdalin Analogues Inhibit IFN-γ Signalling and Reduce the Inflammatory Response in Human Epidermal Keratinocytes

Abstract

Peptide T (PT), an octapeptide fragment located in the V2 region of the HIV-1 gp120-coating protein, appears to be beneficial in the treatment of psoriasis. Our previous investigations suggest that keratinocytes play a key role in conditioning the therapeutic effects of PT in psoriasis. The aim of this study was to explore the effects of PT and the peptidomimetic natural products, Dhurrin and Prunasin, on the expression of the IL-6, IL-8, IL-23, HSP70 and ICAM-1 on IFN-γ and TNF-α-NHEK activated cells. Moreover, we analysed the interference of PT and its analogues through STAT-3 activation. Our results show that the analogues tested exhibit the beneficial biological effects of PT, suggesting the primary role of keratinocytes upon which PT and the peptidomimetics act directly, by reducing proinflammatory responses. Its reduction appears to be important for therapeutic approach in psoriasis pathogenesis.

RETRACTED ARTICLE: Amygdalin reduces lipopolysaccharide-induced chronic liver injury in rats by down-regulating PI3K/AKT, JAK2/STAT3 and NF-κB signalling pathways

“…Recent studies [23] have reported that AG markedly improved glucose tolerance, and ameliorates insulin action in obese and/or diabetic patients by activating a signalling pathway related to glucose metabolism. In addition, a large body of evidence demonstrates that AG plays an important role in enhancing insulin sensitivity and reducing hyperlipidaemia [24]. Therefore, it is reasonable to speculate that this mechanism has the potential to treat acute liver injury in patients.…”

Oral Administration of the Japanese Traditional Medicine Keishibukuryogan-ka-yokuinin Decreases Reactive Oxygen Metabolites in Rat Plasma: Identification of Chemical Constituents Contributing to Antioxidant Activity

“…The prunasin analogues, amygdalin and manderonitrile, were also quantified in plasma at high concentrations. Prunasin has been demonstrated to inhibit the production of pro-inflammatory cytokines by human keratinocytes [ 28 ], implying a possible anti-inflammatory action. The following compounds were also identified in plasma at relatively-high concentrations; tumulosic acid, paeoniflorin, 4- O -methylgallic acid, gallic acid, manderonitrile, and ( E )-cinnamic acid.…”

Xuefu Zhuyu decoction, a traditional Chinese medicine, provides neuroprotection in a rat model of traumatic brain injury via an anti-inflammatory pathway

“…Randomized controlled trials of XFZY have demonstrated valid effects in patients with TBI 36 37 38 . XFZY - derived compounds, including Hydroxysafflor yellow A (HSYA) and Amygdalin, have been shown to possess an ability to control the inflammatory response 39 40 41 . The above evidence also indicates that the potential mechanism of neuroprotection against TBI by XFZY could involve the reduction of the inflammatory cascade.…”