Amygdala opioid receptors mediate the electroacupuncture-induced deterioration of sleep disruptions in epilepsy rats

Yi, Pei-Lu; Chang, Fang‐Chia

doi:10.1016/j.sleep.2013.11.756

Cited by 1 publication

(1 citation statement)

References 26 publications

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“…We previously found that electroacupuncture (EA) with a 10-Hz stimulation frequency, in which a certain amount of current is delivered into the acupoint through a stainless-steel needle, successfully suppresses electrocorticogram (ECoG) epileptic activities and epilepsy-induced sleep disturbances 7 . EA with a 100-Hz stimulation frequency further deteriorates epileptic activities and sleep disruptions in rats 8,9 . This successful experiment depends on three factors: firstly, a feasible epileptic animal model; secondly, a method for sleep recording and analysis in rodents; and thirdly, the accurate performance of acupuncture and the accuracy of the acupoint locations.…”

Section: Introductionmentioning

confidence: 95%

Manipulation of Epileptiform Electrocorticograms (ECoGs) and Sleep in Rats and Mice by Acupuncture

Jou

et al. 2016

JoVE

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Ancient Chinese literature has documented that acupuncture possesses efficient therapeutic effects on epilepsy and insomnia. There is, however, little research to reveal the possible mechanisms behind these effects. To investigate the effect of acupuncture on epilepsy and sleep, several issues need to be addressed. The first is to identify the acupoints, which correspond between humans, rats, and mice. Furthermore, the depth of insertion of the acupuncture needle, the degree of needle twist in manual needle acupuncture, and the stimulation parameters for electroacupuncture (EA) need to be determined. To evaluate the effects of acupuncture on epilepsy and sleep, a feasible model of epilepsy in rodents is required. We administer pilocarpine into the left central nucleus of the amygdala (CeA) to simulate focal temporal lobe epilepsy (TLE) in rats. Intraperitoneal (IP) injection of pilocarpine induces generalized epilepsy and status epilepticus (SE) in rats. Five IP injections of pentylenetetrazol (PTZ) with a one-day interval between each injection successfully induces spontaneous generalized epilepsy in mice. Recordings of electrocorticograms (ECoGs), electromyograms (EMGs), brain temperature, and locomotor activity are used for sleep analysis in rats, while ECoGs, EMGs, and locomotor activity are employed for sleep analysis in mice. ECoG electrodes are implanted into the frontal, parietal, and contralateral occipital cortices, and a thermistor is implanted above the cerebral cortex by stereotactic surgery. EMG electrodes are implanted into the neck muscles, and an infrared detector determines locomotor activity. The criteria for categorizing vigilance stages, including wakefulness, rapid eye movement (REM) sleep, and non-REM (NREM) sleep are based on information from ECoGs, EMGs, brain temperature, and locomotor activity. Detailed classification criteria are stated in the text.

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Section: Introductionmentioning

confidence: 95%

Manipulation of Epileptiform Electrocorticograms (ECoGs) and Sleep in Rats and Mice by Acupuncture

Jou

et al. 2016

JoVE

View full text Add to dashboard Cite

show abstract

Amygdala opioid receptors mediate the electroacupuncture-induced deterioration of sleep disruptions in epilepsy rats

Cited by 1 publication

References 26 publications

Manipulation of Epileptiform Electrocorticograms (ECoGs) and Sleep in Rats and Mice by Acupuncture

Manipulation of Epileptiform Electrocorticograms (ECoGs) and Sleep in Rats and Mice by Acupuncture

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