Amy as a reporter gene for promoter activity in Nocardia lactamdurans: comparison of promoters of the cephamycin cluster

Chary, Vasant K.; Fuente, J. L. de la; Liras, Paloma; Martı́n, Juan F.

doi:10.1128/aem.63.8.2977-2982.1997

Cited by 24 publications

(5 citation statements)

References 23 publications

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“…Two DNA fragments of 1.2 kb Kpn I– Sac II and 3.5 kb Sac I, both containing the tlrB gene (Fig. 1), were purified from the 5.5‐kb Bgl II fragment and subcloned in the bifunctional E. coli – Streptomyces plasmid pULVK99 [14] rendering pALF250 and pALF295, respectively (Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

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Conjugation and transformation of Streptomyces species by tylosin resistance

Fouces¹,

Rodríguez²,

Mellado³

et al. 2000

FEMS Microbiology Letters

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The tlrB gene from Streptomyces fradiae has been cloned and used to construct bifunctional Streptomyces-Escherichia coli shuttle vectors carrying the antibiotic resistance genes to kanamycin-neomycin, thiostrepton and tylosin as selection markers. In the same way, the tlrB gene was subcloned in plasmids including the apramycin resistance gene and the oriT sequence from the plasmid pSET152 to facilitate conjugation of Streptomyces spores. The usefulness of the tlrB gene as tylosin resistance marker was ascertained in Streptomyces lividans, Streptomyces parvulus and Streptomyces coelicolor, but not in Streptomyces clavuligerus. The tlrB gene constitutes a useful selection marker when high-frequency of conjugation/transformation is not required or as secondary marker in recombinant Streptomyces species where thiostrepton and kanamycin have been utilized for primary selection.

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Section: Resultsmentioning

confidence: 99%

“…pBluescript I KS(+), pBluescript II SK(+) and pBC KS(+) phagemids (Stratagene) were utilized for routine subcloning and ssDNA preparation with the helper phage M13K07. pULVK99 [14] was used as E. coli – Streptomyces spp. shuttle vector and pSET152 [10] as intergeneric conjugation vector.…”

Section: Methodsmentioning

confidence: 99%

Conjugation and transformation of Streptomyces species by tylosin resistance

Fouces¹,

Rodríguez²,

Mellado³

et al. 2000

FEMS Microbiology Letters

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show abstract

“…Two DNA fragments of 1.2 kb KpnI^SacII and 3.5 kb SacI, both containing the tlrB gene ( Fig. 1), were puri¢ed from the 5.5-kb BglII fragment and subcloned in the bifunctional E. coli^Streptomyces plasmid pULVK99 [14] rendering pALF250 and pALF295, respectively (Fig. 2).…”

Section: Construction Of Plasmids Carrying the Tlrb Genementioning

confidence: 99%

Conjugation and transformation of Streptomyces species by tylosin resistance

Fouces¹

2000

FEMS Microbiology Letters

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The tlrB gene from Streptomyces fradiae has been cloned and used to construct bifunctional Streptomyces^Escherichia coli shuttle vectors carrying the antibiotic resistance genes to kanamycin^neomycin, thiostrepton and tylosin as selection markers. In the same way, the tlrB gene was subcloned in plasmids including the apramycin resistance gene and the oriT sequence from the plasmid pSET152 to facilitate conjugation of Streptomyces spores. The usefulness of the tlrB gene as tylosin resistance marker was ascertained in Streptomyces lividans, Streptomyces parvulus and Streptomyces coelicolor, but not in Streptomyces clavuligerus. The tlrB gene constitutes a useful selection marker when high-frequency of conjugation/transformation is not required or as secondary marker in recombinant Streptomyces species where thiostrepton and kanamycin have been utilized for primary selection. ß

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“…strain HR167 [16], Amycolatopsis mediterranei U-32 [11] and A. mediterranei strains DSM 40773, DSM 43304, MTCC 17 [4,12,14,17]. Several studies have demonstrated the utility of pA387-derived shuttle vectors [19][20][21][22][23][24]. However, relatively little is known regarding the genetic makeup of plasmid pA387.…”

Section: Introduction *mentioning

confidence: 99%

Nucleotide sequence of plasmid pA387 of Amycolatopsis benzoatilytica and construction of a conjugative shuttle vector

Malhotra¹,

Majumdar²,

Kumar³

et al. 2008

J. Basic Microbiol.

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The complete nucleotide sequence of plasmid pA387 of Amycolatopsis benzoatilytica DSM 43387 was determined. Sequence analysis revealed that pA387 is 30,157 bp long and has a G+C content of 71.74%. To obtain a minimal transferable replicon capable of self-replication, a 2,176 bp fragment of pA387 was cloned, and we demonstrated that this fragment is sufficient for autonomous replication. The replication region of pA387 exhibited no significant homology to any known replication proteins available in databases. Putative maintenance and transfer functions were identified on pA387. The predicted products of open reading frames, ORF 2 and ORF 12, resembled the plasmid stabilizing proteins, a DNA resolvase and a ParA protein, respectively. The putative translational products of ORF 15 and ORF 16 showed similarity to known bacterial conjugation proteins, TraG and TraA, respectively. A conjugative Escherichia coli -Amycolatopsis shuttle-cloning vector was constructed by using the pA387 replicon and designated pSETRL1. Shuttle vector pSETRL1 successfully transformed Amycolatopsis mediterranei DSM 40773 and Amycolatopsis orientalis NBRC 12806 by conjugation and electroporation, and is likely to be a useful vector in Amycolatopsis research.

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Amy as a reporter gene for promoter activity in Nocardia lactamdurans: comparison of promoters of the cephamycin cluster

Cited by 24 publications

References 23 publications

Conjugation and transformation of Streptomyces species by tylosin resistance

Conjugation and transformation of Streptomyces species by tylosin resistance

Conjugation and transformation of Streptomyces species by tylosin resistance

Nucleotide sequence of plasmid pA387 of Amycolatopsis benzoatilytica and construction of a conjugative shuttle vector

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