Amplitude and polarization modulated hyperspectral Stimulated Raman Scattering Microscopy

Andreana, Marco; Houle, Marie-Andrée; Moffatt, Douglas J.; Ridsdale, Andrew; Buettner, Edlef; Légaré, François; Stolow, Albert

doi:10.1364/oe.23.028119

Cited by 30 publications

(24 citation statements)

References 40 publications

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“…This confirms the high spectral resolution achievable with our PTS-SRS instrument. We note the presence of small modulations in the acetone spectrum in the 2800-2850 cm -1 spectral region: since they are consistently reproducible (compare the SRG spectra measured with the 8-bit and the 12-bit oscilloscope) but not present in the spontaneous Raman spectra, we attribute them to crossphase modulation [46,47] between pump and Stokes pulses in the sample. We measured the SRG spectrum of acetone using the PTS-SRS setup at various pump fluences, in order to confirm the achievable sensitivity and the linearity of the signal with the pump intensity.…”

Section: Resultsmentioning

confidence: 64%

Broadband stimulated Raman scattering spectroscopy by a photonic time stretcher

Saltarelli¹,

Kumar²,

Viola³

et al. 2016

Opt. Express

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Stimulated Raman scattering spectroscopy is a powerful technique for label-free molecular identification, but its broadband implementation is technically challenging. We introduce and experimentally demonstrate a novel approach based on photonic time stretch. The broadband femtosecond Stokes pulse, after interacting with the sample, is stretched by a telecom fiber to ≈15ns, mapping its spectrum in time. The signal is sampled through a fast analog-to-digital converter, providing single-shot spectra at 80-kHz rate. We demonstrate ≈10^-5 sensitivity over ≈500cm^-1 in the C-H region. Our results pave the way to high-speed broadband vibrational imaging for materials science and biophotonics.

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Section: Resultsmentioning

confidence: 64%

Broadband stimulated Raman scattering spectroscopy by a photonic time stretcher

Saltarelli¹,

Kumar²,

Viola³

et al. 2016

Opt. Express

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“…However other nonlinear optical processes such as cross-phase modulation, transient absorption and photothermal lensing can interfere with the phase-sensitive SRS detection, though are not typically as detrimental as the non-resonant background in CARS microscopy [47]. Several approaches have been demonstrated to reduce these contributions, such as spectral modulation of the lasers to switch on and off the Raman resonance in single-wavenumber SRS [48] and using polarization modulation combined with standard amplitude modulation for hyperspectral SRS [49]. Thus the interaction of the induced pump/Stokes nonlinear polarizations with the incident pump/Stokes fields leads to intensity gain (ΔI s ) and loss (ΔI p ) given by…”

Section: Srsmentioning

confidence: 99%

“…Thus the choice is usually made depending on the sensitivity of the photodetector at ω p or ω s [242]. However the choice can change the relative background contributions to the SRS signal, for example the two-photon absorption will generally be more prevalent in the SRL (pump beam) signal [49]. Further detailed discussions of instrumentation and noise considerations can be found in Min et al [23] and Zhang et al [22].…”

Section: Typical Systemmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

233

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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“…Raman spectra of individual blood cells are known, for instance, to show spectral features characteristic to tumor cells [37]. Polarization modulation between two polarization states has been used recently in combination with amplitude modulation for optimizing spectral focusing in SRS microscopy, essentially by eliminating cross-phase modulation in isotropic samples [38]. This scheme could be advantageously combined with the present method in non-isotropic samples such as those studied here, in order to increase the robustness of spectral measurement in broadband SRS configurations where polarization sensitivity is required.…”

Section: Discussionmentioning

confidence: 99%

High-speed polarization-resolved coherent Raman scattering imaging

Hofer¹,

Balla²,

Brasselet³

2017

Optica

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Polarization-resolved coherent Raman scattering (polar-CRS) provides rich information on molecular orientational organization, with the strong advantages of being a label-free and chemically specific imaging method. Its implementation, however, strongly reduces the imaging acquisition rate, due to limits imposed by polarization tuning. Here we demonstrate fast-polar-CRS imaging based on combined electro-optic polarization and acousto-optic amplitude modulations, applicable to both stimulated Raman scattering and coherent anti-Stokes Raman scattering imaging. The proposed scheme adds polarization information without compromising the capacities of regular CRS intensity imaging; increases the speed of orientational imaging by two orders of magnitude as compared with previous approaches; and does not require post-processing analyses. We show that this method permits sub-second time-scale imaging of lipid order packing and local lipid membrane deformations in artificial lipid multilayers, but also in red blood cell ghosts, demonstrating its high sensitivity down to a single lipid bilayer membrane.

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Amplitude and polarization modulated hyperspectral Stimulated Raman Scattering Microscopy

Cited by 30 publications

References 40 publications

Broadband stimulated Raman scattering spectroscopy by a photonic time stretcher

Broadband stimulated Raman scattering spectroscopy by a photonic time stretcher

Raman spectroscopy: techniques and applications in the life sciences

High-speed polarization-resolved coherent Raman scattering imaging

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