Amplitude and phase images of cellular structures with a scanning surface plasmon microscope

Berguiga, Lotfi; Roland, Thibault; Monier, Karine; Elezgaray, Juan; Argoul, Françoise

doi:10.1364/oe.19.006571

Cited by 33 publications

(15 citation statements)

References 39 publications

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“…1(a)], only a limited range of azimuthal angle φ contrib utes to the SPPs excitation. Given the radial geometry of the objective lens, all azimuthal angles can excite the SPPs [33,40,43] with a radially polarized beam. In this case, the polarization is purely p. For this purpose, we have introduced a liquid crystal cell polarization converter (PC) [50] to convert a linearly polarized beam into a radially polarized beam [ Fig.…”

Section: A Experimental Setupmentioning

confidence: 99%

Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam

et al. 2016

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We report on a fibered high-resolution scanning surface plasmon microscope for long term imaging of living adherent cells. The coupling of a high numerical aperture objective lens and a fibered heterodyne interferometer enhances both the sensitivity and the long term stability of this microscope, allowing for time-lapse recording over several days. The diffraction limit is reached with a radially polarized illumination beam. Adherence and motility of living C2C12 myoblast cells are followed for 50 h, revealing that the dynamics of these cells change after 10 h. This plasmon enhanced evanescent wave microscopy is particularly suited for investigating cell adhesion, since it can not only be performed without staining of the sample but it can also capture in real time the exchange of extracellular matrix elements between the substrate and the cells.

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Section: A Experimental Setupmentioning

confidence: 99%

Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam

et al. 2016

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“…Devices that replace the prism by oil-immersion [34][35][36] or solid-immersion [37] lenses for coupling light into SPPs circumvent this difficulty by shaping and confining the SPP laterally to the gold surface. The combination of high numerical aperture lenses and interferometric devices pushed further down its resolution to subwavelength distances [38][39][40][41][42][43][44]. Mainly two methods have been proposed: a wide-field SPR microscope (WSPRM) and a scanning SPR microscope (SSPRM).…”

Section: Enhancement Of the Guiding Wave Mechanism By Surface Plasmonmentioning

confidence: 99%

Resonant Waveguide Imaging of Living Systems: From Evanescent to Propagative Light

Argoul¹,

Berguiga²,

Elezgaray³

et al. 2016

Handbook of Photonics for Biomedical Engineering

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International audienceFor more than 50 years, resonant waveguides (RWGs) have offered highly sensitive label-free sensing platforms to monitor surface processes such as protein adsorption, affinity binding, monolayer to multilayer build-up, bacteria and more generally adherent or confined living mammalian cells and tissues. Symmetrical planar dielectric RWG sensitivity was improved by metal coating of at least one of their surfaces for surface plasmon resonance undertaking (SPRWG). However, RWG sensitivity was often obtained at the expense of spatial resolution and could not compete with other high resolution fluorescence microscopies. For years, RWGs have only rarely been combined with high-resolution microscopy. Only recently, the improvement of intensity and phase light modulation techniques and the availability of low-cost high numerical aperture lenses have drastically changed the devices and methodologies based on RWGs. We illustrate in this chapter how these different technical and methodological evolutions have offered new, versatile, and powerful imaging tools to the biological community

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“…In recent years there has been a growing interest in performing surface plasmon (SP) measurements within a microscopic environment [1][2][3][4][5][6]; this offers the possibility of obtaining localized high resolution measurements of local changes in refractive index as well as performing valuable imaging of cell attachment [7][8][9]. We have shown that the lateral resolution of SP microscopy is optimized with an interferometric arrangement and we have more recently demonstrated that similar performance can be achieved with a confocal microscope arrangement [5].…”

Section: Introductionmentioning

confidence: 96%

Surface plasmon microscopic sensing with beam profile modulation

Zhang¹,

Pechprasarn²,

Somekh³

2012

Opt. Express

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Surface Plasmon microscopy enables measurement of local refractive index on a far finer scale than prism based systems. An interferometric or confocal system gives the so-called V(z) curve when the sample is scanned axially, which gives a measure of the surface plasmon propagation velocity. We show how a phase spatial light modulator (i) performs the necessary pupil function apodization (ii) imposes an angular varying phase shift that effectively changes sample defocus without any mechanical movement and (iii) changes the relative phase of the surface plasmon and reference beam to provide signal enhancement not possible with previous configurations.

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Amplitude and phase images of cellular structures with a scanning surface plasmon microscope

Cited by 33 publications

References 39 publications

Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam

Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam

Resonant Waveguide Imaging of Living Systems: From Evanescent to Propagative Light

Surface plasmon microscopic sensing with beam profile modulation

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