Amplitude- and frequency-dependent activation of layer II/III neurons by intracortical microstimulation

Wu, Guangying K.; Ardeshirpour, Yasaman; Mastracchio, Christina; Kent, Jordan; Caiola, Michael; Ye, Meijun

doi:10.1016/j.isci.2023.108140

Cited by 3 publications

(3 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, using two-photon microscopy in mouse somatosensory cortex, Ma et al evaluated neural responses to various stimulation intensities from 25 to 225 µA and found that neural activity amplitude exhibited a sigmoidal profile to the intensity change, in which around 80 µA/phase stimulation pulse train elicited halfmaximum response [28]. Similar intensity-response trends, which exhibit a sigmoidal curve or at least a monotonic increase in neural response along with stimulation intensity increase, were reported in multiple other studies [6,35,59,93,94]. Sombeck et al explored the decrease in neural activity by changing stimulation intensity from 5 to 100 µA and found that such depression duration and fraction of depressed neurons increased along with stimulation intensity increase [94].…”

Section: Effect Of Stimulation Pulse Intensitysupporting

confidence: 53%

“…While many studies have examined the activation of neuronal responses induced by ICMS, fewer studies have characterized the depressive effects of ICMS on the neural response following stimulation [6,31,[33][34][35]. Previous research has demonstrated significant depression of neural activity following single or repetitive ICMS, occurring at various time scales ranging from milliseconds to hours.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Activation and depression of neural and hemodynamic responses induced by the intracortical microstimulation and visual stimulation in the mouse visual cortex

Suematsu,

Vazquez,

Kozai

2024

J. Neural Eng.

View full text Add to dashboard Cite

Objective.Intracortical microstimulation can be an effective method for restoring sensory perception in contemporary brain-machine interfaces. However, the mechanisms underlying better control of neuronal responses remain poorly understood, as well as the relationship between neuronal activity and other concomitant phenomena occurring around the stimulation site. Approach. Different microstimulation frequencies were investigated in vivo on Thy1-GCaMP6s mice using widefield and two-photon imaging to evaluate the evoked excitatory neural responses across multiple spatial scales as well as the induced hemodynamic responses. Specifically, we quantified stimulation-induced neuronal activation and depression in the mouse visual cortex and measured hemodynamic oxyhemoglobin and deoxyhemoglobin signals using mesoscopic-scale widefield imaging.Main results.Our calcium imaging findings revealed a preference for lower-frequency stimulation in driving stronger neuronal activation. A depressive response following the neural activation preferred a slightly higher frequency stimulation compared to the activation. Hemodynamic signals exhibited a comparable spatial spread to neural calcium signals. Oxyhemoglobin concentration around the stimulation site remained elevated during the post-activation (depression) period. Somatic and neuropil calcium responses measured by two-photon microscopy showed similar dependence on stimulation parameters, although the magnitudes measured in soma was greater than in neuropil. Furthermore, higher-frequency stimulation induced a more pronounced activation in soma compared to neuropil, while depression was predominantly induced in soma irrespective of stimulation frequencies. Significance. These results suggest that the mechanism underlying depression differs from activation, requiring ample oxygen supply, and affecting neurons. Our findings provide a novel understanding of evoked excitatory neuronal activity induced by intracortical microstimulation and offer insights into neuro-devices that utilize both activation and depression phenomena to achieve desired neural responses.

show abstract

Section: Effect Of Stimulation Pulse Intensitysupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Activation and depression of neural and hemodynamic responses induced by the intracortical microstimulation and visual stimulation in the mouse visual cortex

Suematsu,

Vazquez,

Kozai

2024

J. Neural Eng.

View full text Add to dashboard Cite

show abstract

“…While many studies have examined the activation of neuronal responses induced by ICMS, fewer studies have characterized the depressive effects of ICMS on the neural response following stimulation [6,29,[31][32][33]. Previous research has demonstrated significant depression of neural activity following single or repetitive ICMS, occurring at various time scales ranging from milliseconds to hours.…”

Section: Introductionmentioning

confidence: 99%

Activation and depression of neural and hemodynamic responses induced by the intracortical microstimulation and visual stimulation in the mouse visual cortex

Suematsu,

Vazquez,

Kozai

2024

Preprint

View full text Add to dashboard Cite

Objective. Intracortical microstimulation can be an effective method for restoring sensory perception in contemporary brain-machine interfaces. However, the mechanisms underlying better control of neuronal responses remain poorly understood, as well as the relationship between the neuronal activity and other concomitant phenomena occurring around the stimulation site. Approach. Different microstimulation frequencies were investigated in vivo on Thy1-GCaMP6s mice using widefield and two-photon imaging to evaluate the evoked excitatory neural responses across multiple spatial scales as well as the induced hemodynamic responses. Specifically, we quantified stimulation-induced neuronal excitation and silencing in the mouse visual cortex and measured hemodynamic oxyhemoglobin and deoxyhemoglobin signals using mesoscopic-scale widefield imaging. Main results. Our calcium imaging findings revealed a preference for lower-frequency stimulation in neuronal excitation. Hemodynamic signals exhibited a similar distance threshold to the neuronal calcium signals with the oxyhemoglobin concentration around the stimulation site remaining elevated after the stimulation train ended. Somatic and neuropil calcium responses measured by two-photon microscopy showed similar dependence on stimulation parameters, although the magnitudes differed significantly. Furthermore, higher-frequency stimulation induced soma-dominant excitation without silencing. Significance. These results suggest that the mechanism underlying silencing differs from the excitation, requiring ample oxygen supply, and affecting neurons. Our findings provide a novel understanding of evoked excitatory neuronal activity induced by intracortical stimulation and offer insights into neuro-devices that utilize both excitation and silencing phenomena to achieve desired neural responses.

show abstract

Inkjet-printed transparent electrodes for electrical brain stimulation

Matta,

Reato,

Lombardini

et al. 2024

Preprint

View full text Add to dashboard Cite

Electrical stimulation is a powerful tool for investigating and modulating brain activity, as well as for treating neurological disorders. However, understanding the precise effects of electrical stimulation on neural activity has been hindered by limitations in recording neuronal responses near the stimulating electrode, such as stimulation artifacts in electrophysiology or obstruction of the field of view in imaging. In this study, we introduce a novel stimulation device fabricated from conductive polymers that is transparent and therefore compatible with optical imaging techniques. The device is manufactured using a combination of microfabrication and inkjet printing techniques and is flexible, allowing better adherence to the brain’s natural curvature. We characterized the electrical and optical properties of the electrode and evaluated its performance in the brain of an anesthetized mouse. Furthermore, we combined experimental data with a finite-element model of the in-vivo experimental setup to estimate the maximum electric field that the highly transparent device can generate in the mouse brain. Our findings indicate that the device can generate an electric field as high as 300 V/m, demonstrating its potential for studying and manipulating neural activity using a range of electrical stimulation techniques relevant to human applications. Overall, this work presents a promising approach for developing versatile new tools to apply and study electrical brain stimulation.

show abstract

Amplitude- and frequency-dependent activation of layer II/III neurons by intracortical microstimulation

Cited by 3 publications

References 59 publications

Activation and depression of neural and hemodynamic responses induced by the intracortical microstimulation and visual stimulation in the mouse visual cortex

Activation and depression of neural and hemodynamic responses induced by the intracortical microstimulation and visual stimulation in the mouse visual cortex

Activation and depression of neural and hemodynamic responses induced by the intracortical microstimulation and visual stimulation in the mouse visual cortex

Inkjet-printed transparent electrodes for electrical brain stimulation

Contact Info

Product

Resources

About