Amplified Tandem Spinach-Based Aptamer Transcription Enables Low Background miRNA Detection

Tang, Xin; Deng, Ruijie; Sun, Yupeng; Ren, Xiaojun; Zhou, Mengxi; Li, Jinghong

doi:10.1021/acs.analchem.8b02471

Cited by 87 publications

(46 citation statements)

References 46 publications

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“…This signal output mechanism was coupled with SERS technology for the detection of miRNA . Similarly, an RNA aptamer termed Spinach can also be amplified by RCA and then bind with fluorescent dye DFHBI molecules for miRNA detection …”

Section: Rca With Functional Nucleic Acidsmentioning

confidence: 99%

Circular Nucleic Acids: Discovery, Functions and Applications

Mohammed-Elsabagh

Paczkowski

et al. 2020

ChemBioChem

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acids (CNAs) are nucleic acid molecules with a closed-loop structure. This feature comes with a number of advantages including complete resistance to exonuclease degradation, much better thermodynamic stability, and the capability of being replicated by a DNA polymerase in a rolling circle manner. Circular functional nucleic acids, CNAs containing at least a ribozyme/DNAzyme or a DNA/RNA aptamer, not only inherit the advantages of CNAs but also offer some unique application opportunities, such as the design of topologycontrolled or enabled molecular devices. This article will begin by summarizing the discovery, biogenesis, and applications of naturally occurring CNAs, followed by discussing the methods for constructing artificial CNAs. The exploitation of circular functional nucleic acids for applications in nanodevice engineering, biosensing, and drug delivery will be reviewed next. Finally, the efforts to couple functional nucleic acids with rolling circle amplification for ultra-sensitive biosensing and for synthesizing multivalent molecular scaffolds for unique applications in biosensing and drug delivery will be recapitulated.[a] Dr.

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Section: Rca With Functional Nucleic Acidsmentioning

confidence: 99%

Circular Nucleic Acids: Discovery, Functions and Applications

Mohammed-Elsabagh

Paczkowski

et al. 2020

ChemBioChem

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“…It is also worth noting that miRNA‐21 concentration in these diluted serum samples was 0.5 p m ; that is, 500 f m . This detection limit is comparable to or even better than some detection methods with complex enzymatic recycling amplification (Table S2) . These results indicate that the proposed assay may be useful for analysis of biological fluid samples such as urine and cell culture media to quantify miRNA targets at the f m level.…”

Section: Resultsmentioning

confidence: 53%

A New One‐Pot Fluorescence Derivatization Strategy for Highly Sensitive MicroRNA Analysis

Zhang

Zhao

et al. 2020

Chemistry A European J

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MicroRNAs (miRNAs)m odulate the expression of over 30 %o fm ammalian genes during development and apoptosis, and abnormale xpression of miRNAs may lead to arange of humanp athologies. Therefore, analysis of miRNAs is valuable for disease diagnostics. In this work, an ovel onepot fluorescence derivatization strategy wasd eveloped for miRNA analysis. The mechanism of the derivatization reaction was explored by using instrumental methods, including liquid chromatography,f luorescence spectroscopy,a nd mass spectrometry.H ighly fluorescent N 6 -ethenoadenine (e-adenine) was formed and detached from the miRNA sequence through the reaction of adenine in nucleic acids with 2-chloroacetaldehyde (CAA) at 100 8C. This is the first experimental evidence that the cooperation of formed e-adenine and water-mediatedh ydrogen-bond interaction between the protona tt he 2'-a nd the oxyanion at 3'-positions stabilized the oxocarbenium significantly,w hich makes the depurinationa nd derivatization of miRNA highly effective. Based on this derivatization strategy,afacile and sensitive highperformancel iquid chromatography method was developed for quantitative assay of miRNAs. In combination with magnetic solid-phase extraction( MSPE), the HPLCm ethod was shown to be useful for the determination of microRNAsa t sub-picomolar level in serum samples.[a] Dr.

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“…Furthermore, recent studies have suggested that CTCs, cfDNAs, and other circulating cancer markers including exosomes are distinct entities; therefore, each of them alone may not be adequate to reveal complete profiles of cancer. [14][15][16][17] Additionally, different isolation and sample processing kits and protocols for each of these cancer marker subtypes have created large variabilities and inconsistences between different settings, which represents a major roadblock to fully realize liquid biopsy's potential to cancer management in the clinic.…”

Section: Introductionmentioning

confidence: 99%