Amplified suspension magnetic bead-based assay for sensitive detection of anti-glycan antibodies as potential cancer biomarkers

Blšáková, Anna; Kveton, Filip; Lorencová, Lenka; Blixt, Ola; Vikartovská, Alica; Kasák, Peter; Tkac, Jan

doi:10.1016/j.aca.2022.339444

Cited by 5 publications

(2 citation statements)

References 34 publications

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“…An innovative Suspension Magnetic-Beadbased Assay (SUMBA) is created for identifying the potential cancer biomarker known as aberrant glycans (AGA). This method is optimized through energy dispersive X-ray spectroscopy and SPR analyses [155]. Magnetic bead-based electrochemical and colorimetric methods can also reportedly detect the sugar units on cancer cell surface, which can be recognized by aptamer modified magnetic beads, then sequestrated ferroceneboronic acid or 4-mercaptophenylboronic acid to decrease the electrochemical signal [156].…”

Section: Magnetic Biosensorsmentioning

confidence: 99%

Recent Trends and Innovations in Bead-Based Biosensors for Cancer Detection

Cheng,

Yang,

Wang

et al. 2024

Sensors

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Demand is strong for sensitive, reliable, and cost-effective diagnostic tools for cancer detection. Accordingly, bead-based biosensors have emerged in recent years as promising diagnostic platforms based on wide-ranging cancer biomarkers owing to the versatility, high sensitivity, and flexibility to perform the multiplexing of beads. This comprehensive review highlights recent trends and innovations in the development of bead-based biosensors for cancer-biomarker detection. We introduce various types of bead-based biosensors such as optical, electrochemical, and magnetic biosensors, along with their respective advantages and limitations. Moreover, the review summarizes the latest advancements, including fabrication techniques, signal-amplification strategies, and integration with microfluidics and nanotechnology. Additionally, the challenges and future perspectives in the field of bead-based biosensors for cancer-biomarker detection are discussed. Understanding these innovations in bead-based biosensors can greatly contribute to improvements in cancer diagnostics, thereby facilitating early detection and personalized treatments.

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Section: Magnetic Biosensorsmentioning

confidence: 99%

Recent Trends and Innovations in Bead-Based Biosensors for Cancer Detection

Cheng,

Yang,

Wang

et al. 2024

Sensors

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“…Magnetic bead-based separation is a technique used for target capture and enrichment through modified magnetic microspheres. This method finds widespread application in immune detection, cell separation, biomacromolecule purification, and molecular biology [8,9]. Magnetic bead coupling offers cost-effectiveness by utilizing only 25% of the normal antigen amount and 50% of the beads compared to the non-magnetic beads [10].…”

Section: Introductionmentioning

confidence: 99%

Separation mixed semen of two individuals using magnetic beads coupled ABH blood group antibody

Yao,

Adnan,

Wang

2023

Electrophoresis

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In sexual assault cases, one of the most common samples collected is a mixed semen stain, which is often found on the vagina, female underwear, or bed sheets. However, it is usually difficult to identify the perpetrator based on this sample alone. One technique that has been developed to address this issue is magnetic bead‐based separation. This method involves using modified magnetic microspheres to capture and enrich specific target cells, in this case, sperm cells. In this study, we utilized magnetic beads coupled with ABH blood group antibody to isolate sperm cells from an individual of a single ABO blood type. Subsequently, polymerase chain reaction amplification and capillary electrophoresis were employed to perform the genotyping the short tandem repeat (STR) loci. This approach allows for the identification of different individuals in a mixed seminal stain sample from two individuals, by first separating sperm cells based on ABH antigen differences and subsequently utilizing autosomal STR typing on the enriched single blood group cells.

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