Amplified impedimetric DNA sensor based on graphene oxide–phenylboronic acid for sensitive detection of bleomycins

Liu, Wei; Zhang, Yunsong; Xia, Zhang; He, Xiaoli; Zhang, Xiaohua; Chen, Jinhua

doi:10.1039/c3nj01614e

Cited by 13 publications

(3 citation statements)

References 55 publications

(65 reference statements)

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“…These results are also in good agreement with the measurement by electrochemical impedance spectroscopy (EIS, see below), which is an effective method to investigate the interfacial properties of modied electrodes. 47,48 Signicant changes and homogeneous enhancement in the electrochemical impedance spectra of the TA/peptide bilayers modied electrode are observed as a function of the number of assembled bilayer (Fig. 4a).…”

Section: Resultsmentioning

confidence: 94%

Tannic acid anchored layer-by-layer covalent deposition of parasin I peptide for antifouling and antimicrobial coatings

Pranantyo

Zhang

et al. 2016

RSC Adv.

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Section: Resultsmentioning

confidence: 94%

Tannic acid anchored layer-by-layer covalent deposition of parasin I peptide for antifouling and antimicrobial coatings

Pranantyo

Zhang

et al. 2016

RSC Adv.

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“…This anchoring behavior induced the increase of the R ct value. The difficulty of electron transfer is mainly due to the electrostatic impulsion between the negatively charged phosphate groups of pre-hybridized DNA and negatively charged [Fe(CN) 6 ] 3-/4- [21]. The DNA substrate fragment subsequently breaking from the pre-hybridized DNA induced a further improvement in the R ct value in the aqueous solution containing Pb 2+ .…”

Section: Basic Characterization Of Nh 2 -Rgomentioning

confidence: 98%

A gold electrode modified with amino-modified reduced graphene oxide, ion specific DNA and DNAzyme for dual electrochemical determination of Pb(II) and Hg(II)

Wang

Zhang

et al. 2015

Microchim Acta

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Multilayered reduced graphene oxide (rGO) was functionalized with amino groups by treatment with nitrogen plasma. Raman spectroscopy showed plasma treatment not to substantially alter the chemical structure of rGO and that a wide range of functional nitrogen groups is evenly incorporated into the carbon lattice. The amino-modified rGO was used to design an electrochemical biosensor in which a DNAzyme, substrate DNA and Pb(II) and Hg(II) binding DNA were immobilized on the amino-rGO placed on a gold electrode. The high concentration of amino groups and the rough surface of the rGO favor DNA immobilization. Heavy metal ions are bound to the surface via specific interaction between DNA and the two ions which are detected by electrochemical impedance spectroscopy at a potential of 0.2 V (vs. Ag/AgCl). The detection limits for Pb(II) and Hg(II) are as low as 7.8 and 5.4 pM, respectively, and the analytical ranges extend from 0.01 to 100 nM. The sensor is highly specific and stable and therefore represents a highly promising tool for use in environmental monitoring.

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“…DNA biosensors are powerful tools for cancer diagnosis and detection [ 1 ] and have been extensively developed by different methods including fluorescence [ 2 ], luminescence [ 3 ], surface plasma resonance spectroscopy [ 4 ] and electrochemistry [ 5 ]. In recent years, electrochemical DNA sensors have attracted increasing attention for their outstanding advantages such as inexpensive instrumentation, low detection limit and simplicity due to the ease of obtaining an electrical signal [ 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Abnormal Anionic Porphyrin Sensing Effect for HER2 Gene Related DNA Detection via Impedance Difference between MWCNTs and Single-Stranded DNA or Double-Stranded DNA

et al. 2018

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Human epidermal growth factor receptor 2 (HER2) is a key tumor marker for several common and deadly cancers. It is of great importance to develop efficient detection methods for its over-expression. In this work, an electrochemical impedance spectroscopy (EIS) method adjustable by anionic porphyrin for HER2 gene detection has been proposed, based on the impedance difference between multi-walled carbon nanotubes (MWCNTs) and DNA. The interesting finding herein is that with the addition of anionic porphyrin, i.e., meso-tetra(4-sulfophenyl)-porphyrin (TSPP), the impedance value obtained at a glass carbon electrode (GCE) modified with MWCNTs and a single stranded DNA (ssDNA), the probe DNA that might be assembled tightly onto MWCNTs through π-π stacking interaction, gets a slight decrease; however, the impedance value from a GCE modified with MWCNTs and a double stranded DNA (dsDNA), the hybrid of the probe DNA with a target DNA, which might be assembled loosely onto MWCNTs for the screening effect of phosphate backbones in dsDNA, gets an obvious decrease. The reason may be that on the one hand, being rich in negative sulfonate groups, TSPP will try to push DNA far away from CNTs surface due to its strong electrostatic repulsion towards DNA; on the other hand, rich in planar phenyl or pyrrole rings, TSPP will compete with DNA for the surface of CNTs since it can also be assembled onto CNTs through conjugative interactions. In this way, the “loosely assembled” dsDNA will be repelled by this anionic porphyrin and released off CNTs surface much more than the “tightly assembled” ssDNA, leading to a bigger difference in the impedance value between dsDNA and ssDNA. Thus, through the amplification effect of TSPP on the impedance difference, the perfectly matched target DNA could be easily determined by EIS without any label. Under the optimized experimental conditions, this electrochemical sensor shows an excellent linear response to target DNA in a concentration range of 2.0 × 10−11–2.0 × 10−6 M with a limit of detection (LOD) of 6.34 × 10−11 M (S/N = 3). This abnormally sensitive electrochemical sensing performance resulting from anionic porphyrin for DNA sequences specific to HER2 gene will offer considerable promise for tumor diagnosis and treatment.

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Amplified impedimetric DNA sensor based on graphene oxide–phenylboronic acid for sensitive detection of bleomycins

Cited by 13 publications

References 55 publications

Tannic acid anchored layer-by-layer covalent deposition of parasin I peptide for antifouling and antimicrobial coatings

Tannic acid anchored layer-by-layer covalent deposition of parasin I peptide for antifouling and antimicrobial coatings

A gold electrode modified with amino-modified reduced graphene oxide, ion specific DNA and DNAzyme for dual electrochemical determination of Pb(II) and Hg(II)

Abnormal Anionic Porphyrin Sensing Effect for HER2 Gene Related DNA Detection via Impedance Difference between MWCNTs and Single-Stranded DNA or Double-Stranded DNA

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