Amplified Fragment Length Polymorphism Diversity of Cultivated White Oyster Mushroom Pleurotus ostreatus

Jusuf, Muhammad

doi:10.4308/hjb.17.1.21

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…Current methods include macroscopic and microscopic visualization, presumptive identification using Marquis', Ehrlich's, and Froehde's reagents, thin layer chromatography, gas chromatography‐mass spectrometry (GC‐MS), and high‐performance liquid chromatography . Mushroom DNA has been differentiated by restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP) techniques . While both of these DNA methods utilize the polymerase chain reaction (PCR), they also require post‐PCR gel electrophoresis to analyze and visualize the PCR results.…”

mentioning

confidence: 99%

Detection and Identification of Psilocybe cubensis DNA Using a Real‐Time Polymerase Chain Reaction High Resolution Melt Assay,

Cowan

Elkins

2017

Journal of Forensic Sciences

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Psilocybe cubensis, or "magic mushroom," is the most common species of fungus with psychedelic characteristics. Two primer sets were designed to target Psilocybe DNA using web-based software and NBCI gene sequences. DNA was extracted from eighteen samples, including twelve mushroom species, using the Qiagen DNeasy Plant Mini Kit. The DNA was amplified by the polymerase chain reaction (PCR) using the primers and a master mix containing either a SYBR Green I, Radiant™ Green, or LCGreen Plus intercalating dye; amplicon size was determined using agarose gel electrophoresis. The PCR assays were tested for amplifiability, specificity, reproducibility, robustness, sensitivity, and multiplexing with primers that target marijuana. The observed high resolution melt (HRM) temperatures for primer sets 1 and 7 were 78.85 ± 0.31°C and 73.22 ± 0.61°C, respectively, using SYBR Green I dye and 81.67 ± 0.06°C and 76.04 ± 0.11°C, respectively, using Radiant™ Green dye.

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Detection and Identification of Psilocybe cubensis DNA Using a Real‐Time Polymerase Chain Reaction High Resolution Melt Assay,

Cowan

Elkins

2017

Journal of Forensic Sciences

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“…Chandra et al (2010) used the RAPD markers to discriminate the eight Pleurotus species of mushrooms and also found variations in banding pattern. The polymorphism produced by 11 RAPD primers except OPQ 6 , OPR 17 and OPP 17 may be due to the base substitution, insertion and deletion or collection of genetic material from different sources (Chopra, 2005;Jusuf, 2010).…”

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confidence: 99%

Morphological and molecular characterization of Oyster mushroom (Pleurotus spp.)

Khan¹,

Nawaz²,

Malik³

et al. 2011

Afr. J. Biotechnol.

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Mushrooms have medicinal as well as nutritive value and extensively used as human food from the time immortal. In order to determine the genetic diversity among Pleurotus species of mushroom using morphological and random amplified polymorphic DNA (RAPD) markers, about seven different species were collected. Five species, naming Pleurotus platypus (P-6), Pleurotus flabelatus (P-7), Pleurotus florida (P-17), Pleurotus ostreatus (P-19) and Pleurotus sajor-caju (P-56) were from Canada and two Pleurotus warm-stram (P-9) and Pleurotus eryngii (P-16) from Philpines. Seven different morphological traits that is, mycelial growth (mm), cap diameter (cm), total yield (kg), moisture contents (%), ash contents (%), nitrogen contents (%) and protein content (%) were recorded. The dendrogram based on morphological data divided seven species in cluster 'A' and 'B' having four and three species, respectively. The dendrogram based on RAPD analysis generated 3 clusters 'A', 'B' and 'C'. Out of 14 random primers, the maximum polymorphism was observed by primers OPL 3 (72.70 %) and OPL 11 (70%). The two species P-56 and P-17 were observed to be most similar having value 86% and constituting a cluster 'A'. The present work revealed that, morphological and RAPD markers showed different pattern of genetic diversity among different Pleurotus species.

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Genetic Aspects and Strategies for Obtaining Hybrids

Singh

Kamal

2017

Edible and Medicinal Mushrooms

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Amplified Fragment Length Polymorphism Diversity of Cultivated White Oyster Mushroom Pleurotus ostreatus

Cited by 4 publications

References 11 publications

Detection and Identification of Psilocybe cubensis DNA Using a Real‐Time Polymerase Chain Reaction High Resolution Melt Assay,

Detection and Identification of Psilocybe cubensis DNA Using a Real‐Time Polymerase Chain Reaction High Resolution Melt Assay,

Morphological and molecular characterization of Oyster mushroom (Pleurotus spp.)

Genetic Aspects and Strategies for Obtaining Hybrids

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