Amplified EQCM-D detection of extracellular vesicles using 2D gold nanostructured arrays fabricated by block copolymer self-assembly

Abstract

Extracellular vesicles (EVs) are routinely released from nearly all cell types as transport vehicles and for cell communication. Crucially, they contain biomolecular content for the identification of health and disease...

Nanoprojectile Secondary Ion Mass Spectrometry Enables Multiplexed Analysis of Individual Hepatic Extracellular Vesicles

“…These observations informed the sequence of labeling steps when preparing samples for NP-SIMS analysis and underscored the need to carefully examine labeling protocols. We note that considerations of steric hindrance are not unique to our sample preparation and EV analysis, and have been reported previously by others. , Additional reports described the need to consider labeling sequencing during multiplexed detection of EV surface markers. , …”

“…We note that considerations of steric hindrance are not unique to our sample preparation and EV analysis, and have been reported previously by others. 46,47 Additional reports described the need to consider labeling sequencing during multiplexed detection of EV surface markers. 48,49 Detecting Individual EVs Using NP-SIMS.…”

Parallel SPR and QCM-D Quantitative Analysis of CD9, CD63, and CD81 Tetraspanins: A Simple and Sensitive Way to Determine the Concentration of Extracellular Vesicles Isolated from Human Lung Cancer Cells

“…The LOD and LOQ values of the EV immunosensor with the QCM-D detector were determined in the same manner as in the case of the EV immunosensor with the SPR detector. To improve the QCM-D sensitivity in the EV detection, the nanostructured quartz crystal can be used …”

“…To improve the QCM-D sensitivity in the EV detection, the nanostructured quartz crystal can be used. 56 …”

“…To improve the QCM-D sensitivity in the EV detection, the nanostructured quartz crystal can be used. 56 Effect of Sample Dilution. It is known that standard methods of EV isolation based on differential centrifugation protocols tend to induce aggregation of EVs in highly concentrated suspensions.…”