Amplified Aptamer‐Based Assay through Catalytic Recycling of the Analyte

Lü, Chunhua; Li, Juan; Lin, Meihua; Wang, Yiwei; Yang, Huang‐Hao; Chen, Xi; Chen, Guonan

doi:10.1002/anie.201002822

Cited by 217 publications

(78 citation statements)

References 39 publications

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“…[12][13][14][15] Many DNA-related enzymes were also involved to introduce functions such as signal amplification. 16,17 Finally, DNA/GO conjugates were used to template materials synthesis such as metal nanoparticles, [18][19][20] and stacked GO sheets. 21 Fundamental studies on the interaction between DNA and GO were also carried out.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing

et al. 2016

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Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the condition of preparation. Sometimes, a further reduction step is intentionally performed to decrease the oxygen content and the resulting material is called reduced GO (rGO). In this work, DNA adsorption and desorption from GO and rGO is systematically compared. Under the same salt concentration, DNA adsorbs slightly faster with a 2.6-fold higher capacity on rGO. At the same time, adsorbed DNA on rGO is more resistant to desorption induced by temperature, pH, urea, and organic solvents. Various lengths and sequences of DNA probes have been tested. When its complementary DNA (cDNA) is added as a model target analyte, the rGO sample has a higher signal-to-background and signalto-noise ratio, while the GO sample has a slightly higher absolute signal increase and faster signaling kinetics. Adsorbed DNAs on GO or rGO are still susceptible to non-specific displacement by other DNA and proteins. Overall, while rGO adsorb DNA more tightly, it allows efficient DNA sensing with an extremely low background signal.

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Section: Introductionmentioning

confidence: 99%

Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing

et al. 2016

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“…GO strongly adsorbs nonstructured single-stranded (ss-DNA), while adsorption of well-folded or double-stranded (ds) DNA is disfavored. Combined with its superior fluorescence quenching ability, a number of sensors have been prepared to detect metal ions, 31,32 small molecules, 13,33,34 proteins, 35 DNA, [36][37][38][39][40][41] and cells. 42 Most of these sensors were designed using a scheme shown in Figure 1A.…”

Section: Introductionmentioning

confidence: 99%

Molecular Beacon Lighting up on Graphene Oxide

2012

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A molecular beacon (MB) is comprised of a fluorophore and a quencher linked by a DNA hairpin. MBs have been widely used for homogeneous DNA detection. In addition to molecular quenchers, many nanomaterials such as graphene oxide (GO) also possess excellent quenching efficiency. Most reported fluorescent sensors relied on DNA probes physisorbed by GO, which may suffer from non-specific probe displacement and false positive signal. In this work, we report the preparation and characterization of a MB using graphene oxide (GO) as quencher, where an amino and FAM (6carboxyfluorescein) dual labeled DNA was covalently attached to GO via an amide linkage. A major challenge was to remove noncovalently attached probes due to strong DNA adsorption by GO. While DNA desorption was favored at low salt, high pH, high temperature, and by using organic solvents, the complementary DNA was required to achieve complete desorption of non-covalently linked DNA probes. The DNA adsorption energy was measured using isothermal titration calorimetry, revealing the heterogeneous nature of GO. The covalent probe has a detection limit of 2.2 nM using a sample volume of 0.05 mL. With 2 mL sample, the detection limit can reach 150 pM. The covalent probe is highly resistant to non-specific probe displacement and will find applications in serum and cellular samples where high probe stability is demanded.

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“…GO has a net negative charge, good dispersion, and excellent stability [20]. It is also well known that singlestranded nucleic acid is soft and loose linear structure, thus adsorption of it onto the GO is stable, due to - stacking effect between the nucleic acid base and GO [23,24]. However, the formation of the double helix nucleic acid structure is rigid, therefore greatly reduces its adsorption capacity by the GO [23,24].…”

mentioning

confidence: 99%

“…It is also well known that singlestranded nucleic acid is soft and loose linear structure, thus adsorption of it onto the GO is stable, due to - stacking effect between the nucleic acid base and GO [23,24]. However, the formation of the double helix nucleic acid structure is rigid, therefore greatly reduces its adsorption capacity by the GO [23,24]. On the other hand, one interesting property of GO is that it can be used as a broad-spectrum fluorescence quenching agent [23][24][25][26][27][28][29].…”

mentioning

confidence: 99%

“…However, the formation of the double helix nucleic acid structure is rigid, therefore greatly reduces its adsorption capacity by the GO [23,24]. On the other hand, one interesting property of GO is that it can be used as a broad-spectrum fluorescence quenching agent [23][24][25][26][27][28][29]. Therefore, fluorophores labeled in single-stranded nucleic acid probe can be effectively quenched by GO through fluorescence resonance energy transfer (FRET) mechanism.…”

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confidence: 99%

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Sensitive monitoring of RNA transcription levels using a graphene oxide fluorescence switch

2012

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Catalytic transfer of genetic information from DNA to RNA is very important in life activities. The unconventional RNA transcription level may be related to the source of genetic diseases. At present, conventional methods for detection of RNA transcripts usually involve cumbersome preparative steps, or require sophisticated laboratory equipments. In this study, we presented a rapid, sensitive nano-detection platform for monitoring of RNA transcript levels. T7 RNA polymerase transcription reaction is employed as the example to test the feasibility of this method. In this design, in vitro synthesized RNA products can be hybridized to the FAM labeled single strand DNA (ssDNA) probes which can be adsorbed onto the graphene oxide (GO) surface. Using GO as the fluorescence switch, excellent capacity of the signal-on fluorescence platform for detection of RNA transcripts level is demonstrated. Transcription levels sensing with this nano-platform achieved a sensitivity of 5 pmol/L transcription template. It is anticipated that current developed RNA transcript nano-detection mode has the potential to be an alternative to the conventional RNA transcript detection methods.

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Amplified Aptamer‐Based Assay through Catalytic Recycling of the Analyte

Cited by 217 publications

References 39 publications

Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing

Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing

Molecular Beacon Lighting up on Graphene Oxide

Sensitive monitoring of RNA transcription levels using a graphene oxide fluorescence switch

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