Amplification of the Chromosomal
            <i>bla</i>
            <sub>CTX-M-14</sub>
            Gene in Escherichia coli Expanding the Spectrum of Resistance under Antimicrobial Pressure

Yoon, Eun-Jeong; Choi, You Jeong; Kim, Dokyun; Won, Dongju; Choi, Jong Rak; Jeong, Seok Hoon

doi:10.1128/spectrum.00319-22

Cited by 7 publications

(7 citation statements)

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“…Note that the two ST38 strain as well as the ST58 strain carried different β-lactamase alleles, potentially indicating that they were unrelated. We then explored the context of the CTX-M-encoding genes further, as they are mostly carried by plasmids [ 31 ], but chromosomal integration has also been previously reported [ 32 ]. Interestingly, it appears that 4 of the 12 strains (PBIO3538, PBIO3541, PBIO3584, and PBIO3707) carried the ESBL gene chromosomally, with three of these strains belonging to the international high-risk clonal lineages ST38 ( n = 2) and ST58 ( n = 1), which is consistent with previous findings [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

“…The occurrence of five clinically relevant representatives belonging to international high-risk clonal lineages among only 12 ESBL-producing strains is remarkable. ST131 has not only been reported as the major E. coli clone in the clinical setting [ 32 ] but is often also found in veterinary, food, and environmental settings [ 52 , 53 ]. While previous studies have frequently found MDR ST58 strains in farm animals and, to a lesser extent, in wild birds [ 54 , 55 ] and as contaminants of retail meat [ 21 ], ST38 has been detected in several animal species, including broilers, rats, and black-headed gulls [ 12 , 56 ], with the latter matching our results.…”

Section: Discussionmentioning

confidence: 99%

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Multidrug-Resistant High-Risk Escherichia coli and Klebsiella pneumoniae Clonal Lineages Occur in Black-Headed Gulls from Two Conservation Islands in Germany

et al. 2022

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Multidrug-resistant (MDR) Enterobacterales, including extended-spectrum β‑lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae, not only emerge in healthcare settings but also in other habitats, such as livestock and wildlife. The spread of these pathogens, which often combine resistance with high-level virulence, is a growing problem, as infections have become increasingly difficult to treat. Here, we investigated the occurrence of ESBL-producing E. coli and K. pneumoniae in fecal samples from two black-headed gull colonies breeding on two nature conservation islands in Western Pomerania, Germany. In addition to cloacal samples from adult birds (n = 211) and their nestlings (n = 99) during the 2021 breeding season, collective fecal samples (n = 29) were obtained. All samples were screened for ESBL producers, which were then subjected to whole-genome sequencing. We found a total of 12 ESBL-producing E. coli and K. pneumoniae consisting of 11 E. coli and 1 K. pneumoniae, and including the international high-risk E. coli sequence types (ST)131, ST38, and ST58. Eight of the investigated strains had a MDR genotype and carried a large repertoire of virulence-associated genes, including the pap operon, which is important for urinary tract infections. In addition, we identified many genes associated with adherence, biofilm formation, iron uptake, and toxin production. Finally, our analysis revealed the close phylogenetic relationship of ST38 strains with genomes originating from human sources, underlining their zoonotic and pathogenic character. This study highlights the importance of the One Health approach, and thus the interdependence between human and animal health and their surrounding environment.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant High-Risk Escherichia coli and Klebsiella pneumoniae Clonal Lineages Occur in Black-Headed Gulls from Two Conservation Islands in Germany

et al. 2022

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“…Although we were not able to determine the location of bla CTX-M in some E. coli isolates in this study due to incomplete assembly, sequence analysis indicates that IS Ecp1 plays an important role in bla CTX-M dissemination among E. coli isolates and facilitates the horizontal transfer of bla CTX-M from plasmids to chromosomes in distinct integration sites. The chromosomal integration of bla CTX-M is increasingly reported in E. coli , K. pneumoniae , Salmonella , Proteus mirabilis and some other species of Enterobacteriaceae with the help of mobile elements ( Huang et al., 2017 ; He et al., 2017 ; Zeng et al., 2022 ; Yoon et al., 2022 ). Chromosomal integration of bla CTX-M seems to be an adaptive evolution in response to antimicrobial pressure ( Yoon et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…The chromosomal integration of bla CTX-M is increasingly reported in E. coli , K. pneumoniae , Salmonella , Proteus mirabilis and some other species of Enterobacteriaceae with the help of mobile elements ( Huang et al., 2017 ; He et al., 2017 ; Zeng et al., 2022 ; Yoon et al., 2022 ). Chromosomal integration of bla CTX-M seems to be an adaptive evolution in response to antimicrobial pressure ( Yoon et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Nasal carriage of CTX-M-55-producing Escherichia coli ST8369 in a healthy cohort in the city of Yangzhou, China

Wang

Jiang²,

Shao³

et al. 2022

Front. Cell. Infect. Microbiol.

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This study aimed to investigate the prevalence and diversity of extended-spectrum β-lactamases (ESBL)-producing Escherichia coli isolates from healthy individuals in a community and to elucidate their dissemination mechanism. Cefotaxime-resistant E. coli were isolated from 95 samples of healthy persons from one community in Yangzhou, China, and were tested for minimal inhibitory concentrations of 14 antimicrobial agents. The isolates were subjected to whole genome sequencing by Illumina Hiseq or PacBio single-molecule real-time sequencing. A total of 30 cefotaxime-resistant E. coli isolates were obtained, carrying blaCTX-M (n=29) or blaDHA (n=1), of which the blaCTX-M-55 (n=19) was the most predominant genotype. One novel blaCTX-M variant blaCTX-M-252 was identified. Thirteen CTX-M-55-producing E. coli isolates belonged to ST8369 from nasal (n=12) or faecal (n=1) samples shared the identical cgMLST type, resistance profiles, resistance genes, plasmid replicons, and a 5,053-bp blaCTX-M-55 structure ΔIS26-ΔISEcp1-blaCTX-M-55-Δorf477-ΔTn2. The blaCTX-M-55 gene was located on IncHI2/ST3 plasmid in E. coli ST8369. The lengths of blaCTX-M/blaDHA-carrying contigs in the remaining 17 E. coli strains ranged from 1,663 to 382,836 bp, located on chromosome (n=4) or plasmids (n=5); the location of the other eight contigs could not be determined due to incomplete assembly. The blaCTX-M was associated with ISEcp1 as previously reported. Nasal colonization of CTX-M-55-producing ST8369 E. coli strains has occurred among healthy individuals in one community. There is a potential risk of antimicrobial resistance dissemination between humans within one community through close contact or environment via aerosols or dust. Therefore, surveillance of nasal carriage of blaCTX-M in communities is warranted to further monitor the spread of the antimicrobial resistance genes in China.

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“…The copyright holder for this preprint this version posted February 7, 2023. ; https://doi.org/10.1101/2023.02.07.527510 doi: bioRxiv preprint thought to confer a major fitness cost and thus be transient in response to antimicrobials, incorporation of blaCTX-M encoding genes into particular chromosomal locations has been found to have a low fitness cost, allowing for stable amplifications across many generations in the absence of antimicrobial pressure (43,44). We hypothesize that the high rates of recurrence observed amongst ESC-R-Ec relative to ESC-S-Ec strains might, in part, be due to blaCTX-M amplifications occurring amongst colonizing strains in the gastrointestinal tract which would allow for their persistence during carbapenem treatment and subsequent re-infection.…”

Section: Esc-r-ec Populationmentioning

confidence: 99%

Temporal Dynamics of Genetically Heterogeneous Extended-Spectrum Cephalosporin ResistantEscherichia coliBloodstream Infections

Shropshire

Strope

Anand

et al. 2023

Preprint

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Extended-spectrum cephalosporin resistant (ESC-R) Escherichia coli (ESC-R-Ec) is an urgent public health threat with clonal complex (CC) 131, phylogroup B2 strains being particularly concerning as the dominant cause of ESC-R-Ec infections. To address the paucity of recent ESC-R-Ec molecular epidemiology data in the United States (US), we used whole genome sequencing (WGS) to fully characterize a large cohort of invasive ESC-R-Ec at a tertiary care cancer center in Houston, Texas collected from 2016-2020. During the study timeframe, there were 1154 index E. coli bloodstream infections (BSIs) of which 389 (33.7%) were ESC-R. Using time series analyses, we identified a temporal dynamic of ESC-R E. coli BSIs (Ec-BSIs), distinct from ESC-susceptible Ec-BSIs, with cases peaking in the last 6 months of the calendar year. WGS of 297 ESC-R Ec-BSI strains revealed that while CC131 strains accounted for ~45% of total infections, the proportion of CC131 strains remained stable across the time-period, and infection peaks were driven by genetically diverse, non-CC131 isolates. BlaCTX-M variants accounted for most beta-lactamases conferring the ESC-R phenotype (89%; 220/248 index ESC-R Ec-BSIs), and amplification of blaCTX-M genes was widely detected in ESC-R Ec-BSI strains, particularly in carbapenem non-susceptible strains and in strains causing recurrent BSIs. BlaCTX-M-55 was significantly enriched within phylogroup A strains, and we identified blaCTX-M-55 plasmid-to-chromosome transmission occurring across non-B2 strains. Our data provide important information regarding the current molecular epidemiology of invasive ESC-R E. coli and provide novel insights into the genetic basis of observed temporal variability for these clinically important pathogens.

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Amplification of the Chromosomal bla _CTX-M-14 Gene in Escherichia coli Expanding the Spectrum of Resistance under Antimicrobial Pressure

Abstract: Increasing prevalence of E. coli producing CTX-M ESBL is a major concern in clinical settings because it significantly limits treatment options. Thus, it is important to keep watching current molecular mechanisms of resistance and the scheme for dissemination.

Cited by 7 publications

References 32 publications

Multidrug-Resistant High-Risk Escherichia coli and Klebsiella pneumoniae Clonal Lineages Occur in Black-Headed Gulls from Two Conservation Islands in Germany

Multidrug-Resistant High-Risk Escherichia coli and Klebsiella pneumoniae Clonal Lineages Occur in Black-Headed Gulls from Two Conservation Islands in Germany

Nasal carriage of CTX-M-55-producing Escherichia coli ST8369 in a healthy cohort in the city of Yangzhou, China

Temporal Dynamics of Genetically Heterogeneous Extended-Spectrum Cephalosporin ResistantEscherichia coliBloodstream Infections

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Amplification of the Chromosomal bla CTX-M-14 Gene in Escherichia coli Expanding the Spectrum of Resistance under Antimicrobial Pressure

Abstract: Increasing prevalence of E. coli producing CTX-M ESBL is a major concern in clinical settings because it significantly limits treatment options. Thus, it is important to keep watching current molecular mechanisms of resistance and the scheme for dissemination.

Cited by 7 publications

References 32 publications

Multidrug-Resistant High-Risk Escherichia coli and Klebsiella pneumoniae Clonal Lineages Occur in Black-Headed Gulls from Two Conservation Islands in Germany

Multidrug-Resistant High-Risk Escherichia coli and Klebsiella pneumoniae Clonal Lineages Occur in Black-Headed Gulls from Two Conservation Islands in Germany

Nasal carriage of CTX-M-55-producing Escherichia coli ST8369 in a healthy cohort in the city of Yangzhou, China

Temporal Dynamics of Genetically Heterogeneous Extended-Spectrum Cephalosporin ResistantEscherichia coliBloodstream Infections

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Resources

About

Amplification of the Chromosomal bla _CTX-M-14 Gene in Escherichia coli Expanding the Spectrum of Resistance under Antimicrobial Pressure