Amplification of SPPS150 and Salmonella typhi DNA with a high throughput oscillating flow polymerase chain reaction device

Abstract

In this paper, a novel oscillating flow polymerase chain reaction ͑PCR͒ device was designed and fabricated to amplify SPPS150 and salmonella typhi. In this new design, the samples are shuttled ͑oscillating flow͒ inside a microfluidic chip to three different temperature zones required for DNA amplification. The amplification cycle time has markedly been reduced as the reagent volume used was only about 25% of that used in conventional PCRs. Bubble formation and adsorption issues commonly associated to chip based PCR were also eliminated. Based on the performance evaluated, it is demonstrated that this oscillating flow PCR has the advantages of both the stationary chamber and continuous flow PCR devices.

The Dropletisation of Bio-Reactions

“…6.3b). Problems such as sample loss and contamination can be prevented to some extent by treating the internal walls of the device with a hydrophobic coating [24,[32][33][34]. However a more reliable prevention against sample loss and contamination is to encapsulate the sample within an aqueous droplet suspended in an immiscible oil carrier fluid (Fig.…”

“…These designs fall into three broad categories: well-based [17][18][19], single-phase continuous flow [20][21][22][23][24] and dropletbased devices [25][26][27][28][29][30]. Droplet-based designs possess several distinct advantages over other designs for the handling of biological samples, most notably for the case of single cell analysis (Fig.…”

Multichannel oscillatory-flow multiplex PCR microfluidics for high-throughput and fast detection of foodborne bacterial pathogens

“…To form a chip with sealed channels, these chips are required to bond with another cover plate. The materials for the cover chips include glass (Bu et al 2003;Wang et al 2005;Hu et al 2006;Sugumar et al 2010), quartz (Baker et al 2003), PDMS (Frey et al 2007), and PMMA (Auroux et al 2003b(Auroux et al , 2004aCheng et al 2005). Each substrate material has different properties and therefore different advantages and disadvantages.…”

“…The microchannel of the oscillatory-flow PCR can be constructed in such substrates as silicon (Bu et al 2003;Wang et al 2005), glass (Sugumar et al 2010), quartz (Baker et al 2003), and various polymers (for example SU-8 (Auroux et al 2003b, 2004a, poly(methylmethacrylate) (PMMA) (Hardt et al 2004;Münchow et al 2005;Cheng et al 2005), cycloolefin copolymer (COC) (Hardt et al 2004;Münchow et al 2005), polydimethylsiloxane (PDMS) (Hu et al 2006;Frey et al 2007;Ugsornrat et al 2008), polycarbonate (PC) (Ohashi et al 2007), and dry film resist (DFR) ). To form a chip with sealed channels, these chips are required to bond with another cover plate.…”

Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc)

“…The two most favoured formats are the stationary chamber [4][5][6][7] and continuous flow PCR devices. 5,[8][9][10] Other formats include hybrid methods combining stationary-continuous flow 11 and integrated electrorheological-fluid actuated micromixer and micropump with microheater arrays. 12 These formats make use of highly heat conductive substrates such as silicon and low sample volume to accelerate the amplification rate.…”