1994
DOI: 10.2183/pjab.70.185
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Amplification of Paulownia Phytoplasma DNA Fragments by Random Primed-PCR.

Abstract: Abstract:Polymerase chain reaction (PCR) was performed to detect genomic differences between healthy and paulownia witches'-broom (PaWB)-affected paulownia plants using fifteen primer-pair combinations derived from five randomly synthesized primers based on the P1-like adhesin gene sequence of Mycoplasma pneumoniae. Upon comparing the PCR product profiles between healthy and PaWB-affected paulownia DNA samples, three types of PCR profiles were observed. Firstly, some bands were amplified from both healthy and … Show more

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Cited by 2 publications
(1 citation statement)
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“…The disease incidence rate of Paulownia at 5 years old was approximately 50-80%, which could reduce 25% of timber production. Primers based on the P1-like adhesion gene sequence of Mycoplasma pneumoniae have been successfully used to detect this disease (Zhong and Hiruki 1994). If PWB disease is found, the host seedlings should be immediately removed.…”
Section: Diseases Of Paulowniamentioning
confidence: 99%
“…The disease incidence rate of Paulownia at 5 years old was approximately 50-80%, which could reduce 25% of timber production. Primers based on the P1-like adhesion gene sequence of Mycoplasma pneumoniae have been successfully used to detect this disease (Zhong and Hiruki 1994). If PWB disease is found, the host seedlings should be immediately removed.…”
Section: Diseases Of Paulowniamentioning
confidence: 99%