Amplification of hsa-miR-191/425 locus promotes breast cancer proliferation and metastasis by targeting DICER1

Zhang, Xiao; Wu, Mingming; Chong, Qing-Yun; Zhang, Weijie; Qian, Pengxu; Zhang, Pengcheng; Qian, Wangsheng; Zhang, Min; Lobie, Peter E.; Zhu, Tao

doi:10.1093/carcin/bgy102

Cited by 50 publications

(31 citation statements)

References 47 publications

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“…Previous studies have reported that several genes are directly targeted by miR-191 in human cancers including TET1, TIMP3, SATB1, and DIECR1 [27–29]. However, we did not detect significant changes in the expression levels of these mRNAs in Huh 7 cells transfected with a miR-191 mimic or in SMMC-7721 cells transfected with a miR-191 siRNA.…”

Section: Resultscontrasting

confidence: 88%

Circular RNA circTRIM33–12 acts as the sponge of MicroRNA-191 to suppress hepatocellular carcinoma progression

et al. 2019

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Background Recently, the dysregulation of circular RNA (circRNA) have been shown to have important regulatory roles in cancer development and progression, including hepatocellular carcinoma (HCC). However, the roles of most circRNAs in HCC are still unknown. Methods The expression of circular tripartite motif containing 33–12 (circTRIM33–12) in HCC tissues and cell lines was detected by qRT-PCR. The role of circTRIM33–12 in HCC progression was assessed by western blotting, CCK-8, flow cytometry, transwell and a subcutaneous tumor mouse assays both in vitro and in vivo. In vivo circRNA precipitation, RNA immunoprecipitation, luciferase reporter assays were performed to evaluate the interaction between circTRIM33–12 and miR-191. Results Here, we found that circTRIM33–12, is downregulated in HCC tissues and cell lines. The downregulation of circTRIM33–12 in HCC was significantly correlated with malignant characteristics and served as an independent risk factor for the overall survival (OS) and recurrence-free survival (RFS) of patients with HCC after surgery. The reduced expression of circTRIM33–12 in HCC cells increases tumor proliferation, migration, invasion and immune evasion. Mechanistically, we demonstrated that circTRIM33–12 upregulated TET1 expression by sponging miR-191, resulting in significantly reduced 5-hydroxymethylcytosine (5hmC) levels in HCC cells. Conclusions These results reveal the important role of circTRIM33–12 in the proliferation, migration, invasion and immune evasion abilities of HCC cells and provide a new perspective on circRNAs in HCC progression. Electronic supplementary material The online version of this article (10.1186/s12943-019-1031-1) contains supplementary material, which is available to authorized users.

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Section: Resultscontrasting

confidence: 88%

Circular RNA circTRIM33–12 acts as the sponge of MicroRNA-191 to suppress hepatocellular carcinoma progression

et al. 2019

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“…MiR-191 dysregulation in different cancers, such as in hepatocellular carcinoma 34 , breast cancer 7 , pancreatic cancer 35 , gastric cancer 10 , different human cell lines of colorectal cancer 36 , and OSCC 13 , has increasingly been detected, which is in agreement with the overexpression of miR-191 in OSCC cases in this study. However, downregulation of miR-191 in follicular adenoma, anaplastic thyroid carcinoma, and papillary thyroid carcinoma 37 and downregulation of miR-191-5p in the renal cell carcinoma cell lines ACHN and 786-O 38 were in conflict with the results of this study.…”

Section: Discussionsupporting

confidence: 88%

“…Therefore, any change in miRNA expression level may have a link to various diseases like cancer. A number of miRNAs function as both tumor suppressors and as oncogenes (oncomiR) 4 , 5 , having been shown to be involved in various cancers including breast cancer 6 - 8 , gastric cancer 9 - 11 , and OSCC 12 - 14 . Contradictory results have been reported about the miRNA expression profiles of OSCC in human societies, and extensive research needs to be conducted before clinical use 15 , 16 .…”

Section: Introductionmentioning

confidence: 99%

miR-155, miR-191, and miR-494 as diagnostic biomarkers for oral squamous cell carcinoma and the effects of Avastin on these biomarkers

Emami

Mohamadnia

Mirzaei

et al. 2020

JKAOMS

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Objectives Oral squamous cell carcinoma (OSCC) is one of the most common types of head and neck cancer. MicroRNAs, as new biomarkers, are recommended for diagnosis and treatment of different types of cancers. Bevacizumab, sold under the trade name Avastin, is a humanized whole monoclonal antibody that targets and blocks VEGF-A (vascular endothelial growth factor A; angiogenesis) and oncogenic signaling pathways. Materials and Methods This study comprised 50 cases suffering from OSCC and 50 healthy participants. Peripheral blood samples were collected in glass test tubes, and RNA extraction was started immediately. Expression levels of miR-155, miR-191, and miR-494 biomarkers in the peripheral blood of OSCC-affected individuals and healthy volunteers in vivo were evaluated using real-time PCR. The influence of Avastin on the expression levels of the aforementioned biomarkers in vitro and in the HN5 cell line was also investigated. Results Expression levels of miR-155, miR-191, and miR-494 in the peripheral blood of individuals affected by OSCC were higher than in those who were healthy. Moreover, Avastin at a concentration of 400 µM caused a decrease in the expression levels of the three biomarkers and a 1.5-fold, 3.5-fold, and 4-fold increase in apoptosis in the test samples compared to the controls in the HN5 cell line after 24, 48, and 72 hours, respectively. Conclusion The findings of this study demonstrate that overexpression of miR-155, miR-191, and miR-494 is associated with OSCC, and Avastin is able to regulate and downregulate the expression of those biomarkers and increase apoptosis in cancerous cells in the HN5 cell line

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“…Through mouse and human cell experiments, Ma et al demonstrated that miR-10b is highly expressed in metastatic BC cells and can mediate cell migration in the process of forward transfer and invasion [ 15 ]. In addition, Zhang et al revealed that the miR-191/425 cluster promotes the growth, invasion, and metastasis of breast tumours in vivo [ 16 ]. These results indicated that miRNAs play an important role in the development of BC.…”

Section: Discussionmentioning

confidence: 99%

Five-mRNA Signature for the Prognosis of Breast Cancer Based on the ceRNA Network

Shi¹,

Lin³

et al. 2020

BioMed Research International

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Background. The purpose of this study was to investigate the regulatory mechanisms of ceRNAs in breast cancer (BC) and construct a new five-mRNA prognostic signature. Methods. The ceRNA network was constructed by different RNAs screened by the edgeR package. The BC prognostic signature was built based on the Cox regression analysis. The log-rank method was used to analyse the survival rate of BC patients with different risk scores. The expression of the 5 genes was verified by the GSE81540 dataset and CPTAC database. Results. A total of 41 BC-adjacent tissues and 473 BC tissues were included in this study. A total of 2,966 differentially expressed lncRNAs, 5,370 differentially expressed mRNAs, and 359 differentially expressed miRNAs were screened. The ceRNA network was constructed using 13 lncRNAs, 267 mRNAs, and 35 miRNAs. Kaplan-Meier (K-M) methods showed that two lncRNAs (AC037487.1 and MIR22HG) are related to prognosis. Five mRNAs (VPS28, COL17A1, HSF1, PUF60, and SMOC1) in the ceRNA network were used to establish a prognostic signature. Survival analysis showed that the prognosis of patients in the low-risk group was significantly better than that in the high-risk group (p=0.0022). ROC analysis showed that this signature has a good diagnostic ability (AUC=0.77). Compared with clinical features, this signature was also an independent prognostic factor (HR: 1.206, 95% CI 1.108−1.311; p<0.001). External verification results showed that the expression of the 5 mRNAs differed between the normal and tumour groups at the chip and protein levels (p<0.001). Conclusions. These ceRNAs may play a key role in the development of BC, and the new 5-mRNA prognostic signature can improve the prediction of survival for BC patients.

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Amplification of hsa-miR-191/425 locus promotes breast cancer proliferation and metastasis by targeting DICER1

Cited by 50 publications

References 47 publications

Circular RNA circTRIM33–12 acts as the sponge of MicroRNA-191 to suppress hepatocellular carcinoma progression

Circular RNA circTRIM33–12 acts as the sponge of MicroRNA-191 to suppress hepatocellular carcinoma progression

miR-155, miR-191, and miR-494 as diagnostic biomarkers for oral squamous cell carcinoma and the effects of Avastin on these biomarkers

Five-mRNA Signature for the Prognosis of Breast Cancer Based on the ceRNA Network

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