Amplification of genomic DNA of arbuscular-mycorrhizal (AM) fungi by PCR using short arbitrary primers

Wyss, Peter; Bonfante, Paola

doi:10.1016/s0953-7562(09)80169-x

Cited by 106 publications

(39 citation statements)

References 20 publications

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“…Small subunit rRNA sequence data for comparison are available now (Simon et al, 1992;Simon et al, 1993) and did confirm the taxonomic classifications based on spore shape. A method for detecting DNA polymorphism by random amplified polymorphic DNA (RAPD) analysis was reported recently (Wyss and Bonfante, 1993). Geosiphon could then be useful for various further studies on AM fungi.…”

Section: Discussionmentioning

confidence: 99%

Geosiphon pyriforme, an Endosymbiotic Association of Fungus and Cyanobacteria: the Spore Structure Resembles that of Arbuscular Mycorrhizal (AM) Fungi

et al. 1994

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The zygomycete Geosiphon pyriforme is the only known endocyanosis of a fungus. The Nostoc spp. filaments are included in photosynthetically active and nitrogen fixing, multinucleated bladders, which grow on the soil surface. The spores of the fungus are white or slightly brownish. They are about 250 μm in diameter and develop singly on hyphal ends or, less frequently, intercalarly. The wall of the spores consists of a thin innermost layer, a laminated inner layer with a thickness of about 10–13 μm, and an evanescent outer layer. The laminated layer is composed of helicoidally arranged microfibrils, and is separated from the evanescent outer layer by a thin electron‐dense sublayer. Polarisation microscopy indicates the occurrence of chitin. Shape and wall ultrastructure of the Geosiphon spores and their cytoplasm resemble that of Glomus spores, but are different from that of other genera of the Glomales and Endogonales. Germination occurs by a single thick hyphal outgrowth directly through the spore wall. Like various AM forming fungi, Geosiphon pyriforme contains endocytic bacteria‐like organisms, which are not surrounded by a host membrane. Our observations indicate that Geosiphon is a potential AM fungus.

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Section: Discussionmentioning

confidence: 99%

Geosiphon pyriforme, an Endosymbiotic Association of Fungus and Cyanobacteria: the Spore Structure Resembles that of Arbuscular Mycorrhizal (AM) Fungi

et al. 1994

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“…One hundred milligrams of plant material, ground under liquid nitrogen, were extracted in 800 {A of a buffer contaming 100 mM Tris-HCl pH 8,5,0,1 mM EDTA, 1 % <v/v) Triton X-100, After vigorous mixing the sample was treated with 40 mg CHELEX 100 Resin (BioRad Laboratories, Munich, Germany), boiled in a water bath for 15min, chilled on ice for 5min and centrifuged at 14000 X g for 5min (modified after Wyss and Bonfante, 1993), Then 600^1 of the supernatant were removed and diluted 100-fold in sterile distilled water. One microlitre of the diluted extract was sufficient for one PCR assay.…”

Section: Methodsmentioning

confidence: 99%

RAPD‐based Molecular Probes for the Blackleg Fungus Leptosphaeria maculans (Phoma lingam): Evidence for Pathogenicity Group‐specific Sequences in tbe Fungal Genomes

Voigt

Schleier

Wöstemeyer

1998

Journal of Phytopathology

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Polymerase chain reaction (PCR) primers, specific for the aggressive pathogenicity group of the rape seed pathogen Leptosphaeria maculans, were obtained according to the following experimental outline: DNA bands that differentiate between aggressive (A) and nonaggressive (NA) isolates were generated by the random amplified polymorphic DNA approach (RAPD). Differential RAPD bands were checked for pathogenicity group-specificity and sequenced by a combination of chemical and dideoxynucleotide chain termination methods. Based on the DNA sequence longer and highly specific primer pairs for conventional PCR under stringent conditions were designed, which were tested on 97 isolates of L, maculans belonging to different pathogenicity groups.

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“…Molecular methods are becoming valuable tools in the identification of mycorrhizal fungi (Henrion et al 1992, Gardes and Bruns 1993, Wyss and Bonfante 1993, Simon et al 1993. Some of these techniques have been applied for the correct identification of truffles during the different phases of their life cycle especially when morphological characters are ambiguous or missing (Lanfranco et al 1993, Henrion et al 1994, Potenza et al 1994, Gandebouef et al 1995, Paolocci et al 1995.…”

Section: Introductionmentioning

confidence: 99%

Identification of ectomycorrhizae from Tuber species by rflp analysis of the its region

et al. 1996

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Polymorphisms of a ribosomal DNA region (ITS) have been analysed using a specific pair of primers, in order to type fruitbodies and ectomycorrhizae of different truffle species. The identification of ectomycorrhizae was obtained by digestion of the PCR products using restriction enzymes. The resultsshow that the strategy used is both suitable and sensitive to characterize the symbiotic fungi from few mycorrhized root tips.

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Amplification of genomic DNA of arbuscular-mycorrhizal (AM) fungi by PCR using short arbitrary primers

Cited by 106 publications

References 20 publications

Geosiphon pyriforme, an Endosymbiotic Association of Fungus and Cyanobacteria: the Spore Structure Resembles that of Arbuscular Mycorrhizal (AM) Fungi

Geosiphon pyriforme, an Endosymbiotic Association of Fungus and Cyanobacteria: the Spore Structure Resembles that of Arbuscular Mycorrhizal (AM) Fungi

RAPD‐based Molecular Probes for the Blackleg Fungus Leptosphaeria maculans (Phoma lingam): Evidence for Pathogenicity Group‐specific Sequences in tbe Fungal Genomes

Identification of ectomycorrhizae from Tuber species by rflp analysis of the its region

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