Amplification of FISH signals using intermittent microwave irradiation for analysis of chromosomal instability in gastric cancer

Kitayama, Yasuhiko; Igarashi, Hisaki; Sugimura, Haruhiko

doi:10.1136/mp.52.6.357

Cited by 24 publications

(23 citation statements)

References 10 publications

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“…The use of an AR approach was initiated based on evidence that formalin-induced modification of protein and of nucleic acid is similar von Hippel 1977a, 1977b;Masuda et al 1999;Shi et al 2001). Experimental evidence was forthcoming in support of this approach, describing remarkable improvement of signal for chromogenic in situ hybridization (CISH) and FISH (Oliver et al 1997;Bull and Harnden 1999;Kitayama et al 1999;Gu et al 2000). In addition, other investigators have demonstrated the effectiveness of heating for extraction of nucleic acids from FFPE tissues.…”

Section: Extraction Of Nucleic Acids and Proteins From Ffpe Tissuesmentioning

confidence: 99%

Antigen Retrieval Immunohistochemistry

Shi

Taylor

2011

J Histochem Cytochem.

230

105

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SummaryAs a review for the 20th anniversary of publishing the antigen retrieval (AR) technique in this journal, the authors intend briefly to summarize developments in AR-immunohistochemistry (IHC)-based research and diagnostics, with particular emphasis on current challenges and future research directions. Over the past 20 years, the efforts of many different investigators have coalesced in extending the AR approach to all areas of anatomic pathology diagnosis and research and further have led to AR-based protein extraction techniques and tissue-based proteomics. As a result, formalin-fixed paraffinembedded (FFPE) archival tissue collections are now seen as a literal treasure of materials for clinical and translational research to an extent unimaginable just two decades ago. Further research in AR-IHC is likely to focus on tissue proteomics, developing a more efficient protocol for protein extraction from FFPE tissue based on the AR principle, and combining the proteomics approach with AR-IHC to establish a practical, sophisticated platform for identifying and using biomarkers in

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Section: Extraction Of Nucleic Acids and Proteins From Ffpe Tissuesmentioning

confidence: 99%

Antigen Retrieval Immunohistochemistry

Shi

Taylor

2011

J Histochem Cytochem.

230

105

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“…The heat-induced retrieval approach has also been applied to in situ hybridization (ISH), TUNEL [terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling], and FISH (fluorescence in situ hybridization)] (Bull and Harnden 1999;Kitayama et al 1999;Gu et al 2000;Lucassen et al 2000). For multiple IHC staining, heat was used between each round of the immunostaining procedure to block crossreactivity in addition to retrieval of antigenicity (Lan and Nikolic-Paterson 2000).…”

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confidence: 99%

Antigen Retrieval Techniques

Shi

Côté

Taylor

2001

J Histochem Cytochem.

342

248

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S U M M A R Y Development of the antigen retrieval (AR) technique, a simple method of boiling archival paraffin-embedded tissue sections in water to enhance the signal of immunohistochemistry (IHC), was the fruit of pioneering efforts guided by the philosophy of rendering IHC applicable to routine formalin-fixed, paraffin-embedded tissues for wide application of IHC in research and clinical pathology. On the basis of thousands of articles and many reviews, a book has recently been published that summarizes basic principles for practice and further development of the AR technique. Major topics with respect to several critical issues, such as the definition, application, technical principles, and further studies of the AR technique, are highlighted in this article. In particular, a further application of the heat-induced retrieval approach for sufficient extraction of nucleic acids in addition to proteins, and standardization of routine IHC based on the AR technique in terms of a test battery approach, are also addressed. Furthermore, understanding the mechanism of the AR technique may shed light on facilitating the development of molecular morphology. Immunohistochemistry (IHC) has created a wide field for functional (analytical or molecular) morphology, particularly since it has rendered immunoperoxidase methods applicable to routine formalin-fixed, paraffin-embedded tissues based on a series of technical developments. These include increasingly sensitive detection systems and several pretreatments before the immunostaining procedure to recover antigenicity masked by formalin fixation. However, the growing interest of pathologists who attempt further to expand the application of IHC staining on formalin-fixed, paraffin-embedded tissue sections were frustrated by inconsistent results on fixed tissues. More than two decades ago, various alternative fixatives were tried in an attempt to replace formalin in an irreversible chemical reaction of formalin-protein, but they have failed, and it is likely that an ideal fixative will never be found (Larsson 1988). On the other hand, early IHC methods, including initial unmasking techniques such as enzymatic digestion, failed to yield satisfactory immunostaining for many antigens. Therefore, the search for a simple and effective retrieval method has become a hot topic in IHC since the early 1970s (Taylor and Cote 1994). In response to the need for a more effective method to recover the formalin-modified antigenicity, a high-temperature-heating antigen retrieval (AR) technique, the method of boiling paraffin tissue sections in water, was shown 10 years ago to rendering IHC staining possible on archival formalin-fixed, paraffin-embedded tissue sections (Shi et al. 1991). This AR technique was promptly accepted and employed by pathologists and morphologists worldwide, serving as a simple and effective method to achieve satisfactory immunostaining on archival tissue sections, enthusiastically described as "a revolutionary new technique" and "breakthrough in pathology" (Gown et ...

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“…5) [16]. We have also demonstrated that clearer probe-labeling of chromosome territories in FISH studies could be obtained on paraffin sections prepared by QF method without microwave treatment, though such probe-labeling was not always successful even with microwave treatment on paraffinembedded sections prepared by routine aldehyde fixation and alcohol-dehydration method [12,13,16]. These positive effects of QF could be obtained by conventional QF methods for resected tissues and also in the in vivo cryotechnique for living animal organs.…”

Section: Significance Of Cryotechniques For Immunohistochemistrymentioning

confidence: 86%

Advanced Application of the In Vivo Cryotechnique to Immunohistochemistry for Animal Organs

Ohno

Terada

Ohno

2004

Acta Histochem. Cytochem.

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The quick-freezing method often used for physical fixation also contributed enormously to the advancement of morphology, but it could not provide enough information about the dynamic morphological changes in vivo in living animal organs. Therefore, we developed the in vivo cryotechnique in 1995, which could directly cryofix organs in vivo under anesthetized conditions without stopping blood circulation or producing effects of anoxia. We have already reported new findings about the in vivo ultrastructures of living animal organs with the cryotechnique followed by freeze-substitution or replica preparation. Recently, it has also been applied to other morphological analyses in vivo, including immunohistochemistry and FISH, for obtaining dynamically functioning structures of cells and tissues at a light microscopic level. In such experimental processes, the in vivo cryotechnique has been shown to have the added benefit of direct antigen-retrieval effects since it reduces several steps of retrieval treatments which are required in common paraffin-embedded samples prepared by the conventional fixation and dehydration. In conclusion, the in vivo cryotechnique allows us to investigate the functioning real morphology of living animals, which was technically difficult to be observed before, and perform dynamic immunohistochemistry of cells and tissues in various animal organs in vivo at ultimate time-resolution.

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Amplification of FISH signals using intermittent microwave irradiation for analysis of chromosomal instability in gastric cancer

Cited by 24 publications

References 10 publications

Antigen Retrieval Immunohistochemistry

Antigen Retrieval Immunohistochemistry

Antigen Retrieval Techniques

Advanced Application of the In Vivo Cryotechnique to Immunohistochemistry for Animal Organs

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