1988
DOI: 10.1128/jvi.62.9.3288-3294.1988
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Amplification of Epstein-Barr virus (EBV) DNA by superinfection with a strain of EBV derived from nasopharyngeal carcinoma

Abstract: Epstein-Barr virus (EBV) from a nasopharyngeal carcinoma (NPC) hybrid cell line (NPC-KT) lacking defective viral DNA molecules superinfected Raji cells and induced EBV early antigens (EA), as did virus from P3HR-1 cells, which contained defective molecules. The EBV polypeptides induced by NPC-KT appeared to be identical to those induced by P3HR-1 virus. The ability of NPC-KT virus to induce EA was enhanced more than 10-fold by treatment of superinfected cells with dimethyl sulfoxide; however, dimethyl sulfoxid… Show more

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Cited by 10 publications
(3 citation statements)
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“…There are three possible explanations for these results: (1) the 2a-F1 EBV genotype may have infected and transformed a precursor B-lymphocyte, prior to immunoglobulin gene rearrangement, that subsequently gave rise to subclonal populations with different immunoglobulin gene structures [Katamine et al, 1984]. In this case, the dual-EBV-infected tumors would represent multi-focal monoclonal expansion of one of these subclonal cells after subsequent superinfection by the 2a-G EBV genotype [Sato et al, 1988]. (2) The dual-EBV-infected monoclonal tumor may be present in the spleen in low Fig.…”
Section: Molecular Markers Of Tumor Identitymentioning
confidence: 99%
“…There are three possible explanations for these results: (1) the 2a-F1 EBV genotype may have infected and transformed a precursor B-lymphocyte, prior to immunoglobulin gene rearrangement, that subsequently gave rise to subclonal populations with different immunoglobulin gene structures [Katamine et al, 1984]. In this case, the dual-EBV-infected tumors would represent multi-focal monoclonal expansion of one of these subclonal cells after subsequent superinfection by the 2a-G EBV genotype [Sato et al, 1988]. (2) The dual-EBV-infected monoclonal tumor may be present in the spleen in low Fig.…”
Section: Molecular Markers Of Tumor Identitymentioning
confidence: 99%
“…As described above, the RI template for NPC-KT virus DNA apparently contains a single joined terminal fragment of 9.4 kb representing four copies of TR (25). Upon entry into cells, NPC-KT virus circularized to form fused termini ranging in size from 8.4 to 10.4 kb, representing two to six copies of TR.…”
Section: Identification Of Terminal Fragments During Replicationmentioning
confidence: 99%
“…In order to examine the terminal structure of EBV DNA synthesized in IUdR-induced cl.S61 cells, Southern blot analysis was performed with the XhoI 1.9-kilobase (kb) probe, which represented unique DNA adjacent to the repeat sequence at the right terminus (12). The right-terminal BamHI fragment of EBV DNA contained approximately 3.5 kb of unique DNA and various numbers of 0.5-kb terminal repeats.…”
mentioning
confidence: 99%