Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats

Gupta, Manju; Chyi, Y.-S.; Romero‐Severson, Jeanne; Owen, Judith L.

doi:10.1007/bf00224530

Cited by 464 publications

(280 citation statements)

References 29 publications

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“…The microsatellite sequence is used as a single primer in PCR reaction to amplify multiple fragments of different size randomly distributed throughout the genome (Zietkiewicz et al 1994). The primers are usually between 16 and 25 bp long and could either be anchored at 3 0 or 5 0 end or also unanchored (Gupta et al 1994;Meyer et al 1993;Wu et al 1994;Zietkiewicz et al 1994). The main advantages of ISSR are: no need for any prior knowledge of genome sequence, low operational cost, low labor-intensity, high stability and reproducibility, and abundance of genomic information (Moreno et al 1998;Shen et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity among Polish landraces of common oat (Avena sativa L.)

Boczkowska

Tarczyk

2013

Genet Resour Crop Evol

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All over the world about 220,000 accessions of the genus Avena is preserved in gene banks. Polish oats collection, in the National Centre for Plant Genetic Resources, consists of about 2,500 accessions. More than 80 % of them belong to the species Avena sativa. Only 136 accessions have the landrace or traditional cultivar status and 91 of them were collected in Poland. The main objective of this study was to estimate genetic diversity of a set of 67 Polish landraces using inter simple sequence repeat (ISSR) markers. We also tried to determine whether genetic diversity depended on the region and altitude of collection site and if there was a relationship between DNA polymorphism and some morphological traits. The accessions were collected between years 1973 and 1999 from areas of Northern, Southern and Eastern Poland. The obtained results indicated a relatively low diversity (0.09-0.37) of the common oat landraces. Analysis of molecular variation (AMOVA) showed that there were no significant differences within designated geographic regions. It indicated that at the same time the altitude of collection site had significant effect on the genetic differentiation of the accessions. AMOVA also showed the presence of variance between groups formed on the basis of the colour of lemma. White lemma accessions were also characterized by highest genetic distinctiveness. Considering the evident impact of local climatic conditions on the genetic distinctiveness of Polish landraces utility of them in the Polish breeding programs should be considered.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity among Polish landraces of common oat (Avena sativa L.)

Boczkowska

Tarczyk

2013

Genet Resour Crop Evol

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“…has been published by Sharon et al (2006). Other dominant molecular markers that have been used in recent fingerprinting studies for R. solani are universal rice primers (URP) (Kang et al 2002) and inter simple sequence repeats (ISSR) (Gupta et al 1994). Despite some limitations inherent with their dominant nature, the aforementioned markers have been extensively used for eukaryotic organisms because they are cost effective, highly reproducible, have multiple polymorphic loci, are appropriate for closely related individuals and no prior knowledge of the genome is needed for their use (Gupta et al 1994;Nybom 2004;Sharma et al 2005;Xu 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Other dominant molecular markers that have been used in recent fingerprinting studies for R. solani are universal rice primers (URP) (Kang et al 2002) and inter simple sequence repeats (ISSR) (Gupta et al 1994). Despite some limitations inherent with their dominant nature, the aforementioned markers have been extensively used for eukaryotic organisms because they are cost effective, highly reproducible, have multiple polymorphic loci, are appropriate for closely related individuals and no prior knowledge of the genome is needed for their use (Gupta et al 1994;Nybom 2004;Sharma et al 2005;Xu 2006). DNA fingerprinting complemented with another independent criteria such as mycelial compatibility pattern has been suggested for providing preliminary information on mating system and genetic structure of R. solani (Cubeta and Vilgalys 1997;Vilgalys and Cubeta 1994).…”

Section: Introductionmentioning

confidence: 99%

Assessing genetic diversity in the web blight pathogen Thanatephorus cucumeris (anamorph = Rhizoctonia solani) subgroups AG-1-IE and AG-1-IF with molecular markers

Godoy‐Lutz

Steadman

et al. 2012

J Gen Plant Pathol

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genetic diversity in the web blight pathogen Thanatephorus cucumeris (anamorph = Rhizoctonia solani) subgroups AG-1-IE and AG-1-IF with molecular markers" (2012). Papers in Plant Pathology. 273. http://digitalcommons.unl.edu/plantpathpapers/273 Abstract Web blight, an important foliar disease of dry beans in the Americas, is a challenge to manage. We studied genetic variation of 92 isolates of Rhizoctonia solani subgroups AG-1-IE and AG-1-IF using DNA fingerprinting methods and mycelial compatibility grouping. The isolates were collected over 13 years from bean fields in the Dominican Republic, Honduras and Puerto Rico. Cluster and AMOVA analysis of combined data from two universal rice primers and two internal sequence repeats revealed significant genetic variation among and within populations of both subgroups. Variation was influenced by geographic origin and sampling year for AG-1-IE isolates and geographic origin for AG-1-IF isolates. Mycelial compatibility of paired isolates was mostly scored as incompatible in both subgroups and supported many unique phenotypes. Only two isolates of AG-1-IE displayed mycelial compatibility and DNA fingerprints, suggesting clonal origin. Genetic variation in these AG-1-IE and AG-1-IF isolate populations may explain the lack of durable resistance to web blight reported in dry beans.

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“…The genetic diversity of S. homoeocarpa populations on the fairway and putting green at the O. J. Noer field in 2005 was analyzed using inter-simple sequence repeat (ISSR) markers (10,27). A total of 52 and 69 isolates were randomly selected as a representative of the populations on WIGreen and WI-Fairway (sampling 1).…”

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confidence: 99%

Rapid Development of Fungicide Resistance by Sclerotinia homoeocarpa on Turfgrass

Jo¹,

Chang²,

Boehm³

et al. 2008

Phytopathology®

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Dollar spot, caused by Sclerotinia homoeocarpa, is the most prevalent and economically important turfgrass disease in North America. Increasing levels of fungicide resistance, coupled with tightening environmental scrutiny of existing fungicides, has left fewer options for managing dollar spot. More knowledge about S. homoeocarpa populations is needed to improve dollar spot management strategies, especially with respect to minimizing the development of fungicide resistance. Population diversity of S. homoeocarpa was examined using inter-simple sequence repeat markers and vegetative compatibility assays. Two subgroups were found in S. homoeocarpa field populations on both fairway and putting green turfgrass at a research field in Wisconsin. These subgroups were genetically different, vegetatively incompatible, and had different fungicide sensitivities. The frequency of the two genetic subgroups differed significantly between the fairway and putting green, but was uniform within the fairway or within the green. Population dynamics of S. homoeocarpa in response to two systemic fungicides (thiophanate-methyl and propiconazole) were assessed based on in vitro fungicide sensitivity. Dynamics of S. homoeocarpa populations depended on the presence of fungicide-resistant isolates in the initial populations before fungicide applications and changed rapidly after fungicide applications. Shifting of the population toward propiconazole resistance was gradual, whereas thiophanate-methyl resistance developed rapidly in the population. In conclusion, field populations of S. homoeocarpa containing genetically distinct, vegetatively incompatible groups were different on turfgrass that was managed differently, and they were changed rapidly after exposure to fungicides.

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Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats

Cited by 464 publications

References 29 publications

Genetic diversity among Polish landraces of common oat (Avena sativa L.)

Genetic diversity among Polish landraces of common oat (Avena sativa L.)

Assessing genetic diversity in the web blight pathogen Thanatephorus cucumeris (anamorph = Rhizoctonia solani) subgroups AG-1-IE and AG-1-IF with molecular markers

Rapid Development of Fungicide Resistance by Sclerotinia homoeocarpa on Turfgrass

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