Amplification-free CRISPR-Cas13a assay for detection of Taura syndrome virus

Liu, Jianying; Li, Ye; Cao, Hubei; Yao, Sisi; Hu, Keshun; Zhao, Qian; He, Runzhen; Zhu, Ning; Yu, Xiaoping; Fang, Shaohua; Huang, Jun

doi:10.1016/j.aqrep.2023.101552

Cited by 1 publication

(4 citation statements)

References 29 publications

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“…Cas13‐based diagnostics, known as Specific High Sensitivity Enzymatic Reporter unLOCKing (SHERLOCK), initially detected the White spot syndrome virus (WSSV) with sensitivity up to 2 femtograms of DNA 133 . SHERLOCK has been used for various aquaculture pathogens since then 134,135 . Although SHERLOCK targets both DNA and RNA, its popularity in aquaculture is limited due to its qualitative nature 136 .…”

Section: Confirmatory Diagnostic Methodsmentioning

confidence: 99%

“…Integrating RPA into the diagnostic assay for white spot syndrome virus (WSSV) significantly increased t detection by almost five million‐fold 138 . Some researchers explored pre‐amplification‐free CRISPR‐Cas systems for disease diagnosis to minimise pre‐amplification equipment, 135,139 limited to high target amounts.…”

Section: Confirmatory Diagnostic Methodsmentioning

confidence: 99%

“…133 SHERLOCK has been used for various aquaculture pathogens since then. 134,135 Although SHERLOCK targets both DNA and RNA, its popularity in aquaculture is limited due to its qualitative nature. 136 Nonetheless, SHERLOCK shows potential as a field-deployable test kit for disease detection in remote areas with limited access to laboratory facilities.…”

Section: Clustered Regularly Interspaced Short Palindromic Repeats-ba...mentioning

confidence: 99%

“…137 Integrating RPA into the diagnostic assay for white spot syndrome virus (WSSV) significantly increased t detection by almost five million-fold. 138 Some researchers explored pre-amplification-free CRISPR-Cas systems for disease diagnosis to minimise pre-amplification equipment, 135,139 can be measured using mass spectrometer. 150 In MALDI-TOF mass spectrometry, the target samples are mixed with a matrix and passed through high voltage to separate the ions based on their massto-charge ratio, followed by detection using a time-of-flight (TOF)…”

Section: Clustered Regularly Interspaced Short Palindromic Repeats-ba...mentioning

confidence: 99%

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Emerging technologies revolutionising disease diagnosis and monitoring in aquatic animal health

Bohara,

Joshi,

Acharya

et al. 2023

Reviews in Aquaculture

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In recent years, aquaculture has seen tremendous growth worldwide due to technological advancements, leading to research and development of various innovations. Aquaculture farmers prioritise early diagnosis for timely treatment to achieve better productive and economic performance. Aquatic animal health experts still employ traditional diagnostic methods using visual diagnosis, cell culture, media culture, histopathology and serology. However, the developments of technologies in aquamedicine, such as sequencing, biosensors and CRISPR, have enabled rapid disease detection within minutes. Furthermore, integrating sensors, drones, artificial intelligence and the internet in aquaculture farm monitoring has helped farmers take decisive actions to improve production. Advancements in diagnostic techniques have significantly enhanced the efficient detection of bacterial, viral, parasitic and fungal diseases in aquatic animals. Moreover, monitoring water quality, aquatic animal health and animal behaviour on farms has become exceptionally streamlined with cutting‐edge tools like drones, sensors and artificial intelligence. Summarising research and development in aquatic animal health and monitoring aids efficient technology adoption in aquaculture. With these advanced technologies' continued development and adoption in developed countries, the aquaculture industry is experiencing growth and increased efficiency, benefiting farmers and consumers in these regions. However, farmers and educators in developing countries lack information about these technologies. Training of agricultural educators and efficient dissemination of knowledge and technologies through advertising and publication in collaboration with companies is essential. This review delves into emerging technologies capable of replacing the conventional diagnostic and monitoring methods utilised in aquaculture. We also explore their strengths, limitations and potential future applications within aquaculture settings.

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Section: Confirmatory Diagnostic Methodsmentioning

confidence: 99%

Section: Confirmatory Diagnostic Methodsmentioning

confidence: 99%