Amplification and sequencing of Trichuris trichiura ancient DNA extracted from archaeological sediments

Oh, Chang Seok; Seo, Min; Chai, Jong Yil; Lee, Sang Jun; Kim, Myeung Ju; Park, Jun Bum; Shin, Dong Hoon

doi:10.1016/j.jas.2009.12.029

Cited by 49 publications

(33 citation statements)

References 17 publications

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“…Briefly, in our previous studies on the ancient feces, we have employed microscopic or molecular techniques to discover different kinds of ancient parasite eggs such as Ascaris, Trichuris, Clonorchis, Paragonimus, Metagonimus, Gymnophalloides, Strongyloides, Enterobius, Taenia, and Trichostrongylus (Seo et al, 2007, 2008, 2010, 2014aShin et al, 2009aShin et al, , b, 2011Shin et al, , 2012aShin et al, , b, 2013aShin et al, , b, 2014Shin et al, , 2015Oh et al, 2010aOh et al, , b, 2015Kim et al, 2014). From a series of studies on Korean mummy feces, we constructed the existing corpus on Joseon mummy parasitism (n = 18).…”

Section: Introductionmentioning

confidence: 99%

Estimation of parasite infection prevalence of Joseon people by paleoparasitological data updates from the ancient feces of pre-modern Korean mummies

Seo

Hong

et al. 2017

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In paleoparasitology, which is the study of ancient parasite species, parasite egg remnants in archaeological samples are examined by microscopic or molecular analysis. The parasitological information thus obtained can inform speculation about the parasite-infection patterns that prevailed in ancient societies. The current analysis of ancient feces removed from Joseon period mummies adds six new paleoparasitological outcomes to the existing pool of mummy parasitism data already maintained in South Korea. The current microscopic examination revealed the ancient parasite eggs of Trichuris, Clonorchis, Paragonimus, Ascaris, and Taenia in the Joseon mummy feces. When the updated Joseon data were compared with the 20th-century National Survey statistics of South Korea, clear differences could be observed between ancient and modern parasite infection rates. These results will yield invaluable insights-unobtainable by conventional historical investigation-that contribute to the knowledge base on the parasitism of pre-industrial East Asian societies.

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Section: Introductionmentioning

confidence: 99%

Estimation of parasite infection prevalence of Joseon people by paleoparasitological data updates from the ancient feces of pre-modern Korean mummies

Seo

Hong

et al. 2017

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“…If coprolites are being analysed, then fecal bile acids and sterols can help identify whether they are from humans, carnivore or herbivore animals (Shillito et al 2011;Baeten et al 2012). The ancient DNA of parasites can also be studied to differentiate species that appear similar on microscopy (Leles et al 2008;Oh et al 2010). However, this has yet to be undertaken on Roman period parasites.…”

Section: How To Study Ancient Parasites (Palaeoparasitology)mentioning

confidence: 99%

Human parasites in the Roman World: health consequences of conquering an empire

Mitchell

2016

Parasitology

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Roman era is presented in order to demonstrate the species present at that time, and highlight the health consequences for people living under Roman rule. Despite their large multi-seat public latrines with washing facilities, sewer systems, sanitation legislation, fountains and piped drinking water from aqueducts, we see the widespread presence of whipworm (Trichuris trichiura), roundworm (Ascaris lumbricoides) and Entamoeba histolytica that causes dysentery. This would suggest that the public sanitation measures were insufficient to protect the population from parasites spread by fecal contamination. Ectoparasites such as fleas, head lice, body lice, pubic lice and bed bugs were also present, and delousing combs have been found. The evidence fails to demonstrate that the Roman culture of regular bathing in the public baths reduced the prevalence of these parasites. Fish tapeworm was noted to be widely present, and was more common than in Bronze and Iron Age Europe. It is possible that the Roman enthusiasm for fermented, uncooked fish sauce (garum) may have facilitated the spread of this helminth. Roman medical practitioners such as Galen were aware of intestinal worms, explaining their existence and planning treatment using the humoural theory of the period.

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“…The amplification of a partial sequence of the SSU rRNA gene described by Jiang et al (2005) was used for Cryptosporidium parasites. Single PCR amplification was carried out for amplification of the SSU rRNA gene of Trichuris (Oh et al 2010) and for Ascaris sp. was used a primer set flanking the cytochrome b fragment described by Loreille et al (2001).…”

Section: Parasitological Methodsmentioning

confidence: 99%

Paleopathological, Trichological and Paleoparasitological Analysis of Human Skeletal Remains from the Migration Period Cemetery Prague-Zličín

Vargová¹,

Horáčková²,

Horáková³

et al. 2016

IANSA

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Amplification and sequencing of Trichuris trichiura ancient DNA extracted from archaeological sediments

Cited by 49 publications

References 17 publications

Estimation of parasite infection prevalence of Joseon people by paleoparasitological data updates from the ancient feces of pre-modern Korean mummies

Estimation of parasite infection prevalence of Joseon people by paleoparasitological data updates from the ancient feces of pre-modern Korean mummies

Human parasites in the Roman World: health consequences of conquering an empire

Paleopathological, Trichological and Paleoparasitological Analysis of Human Skeletal Remains from the Migration Period Cemetery Prague-Zličín

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