AMPKγ3 is dispensable for skeletal muscle hypertrophy induced by functional overload

Riedl, Isabelle; Osler, Megan E.; Björnholm, Marie; Egan, Brendan; Nader, Gustavo A.; Chibalin, Alexander V.; Zierath, Juleen R.

doi:10.1152/ajpendo.00387.2015

Cited by 9 publications

(23 citation statements)

References 41 publications

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“…Although both groups had comparable height and lean mass in the upper extremities, the overweight subjects had ~10% greater lean mass in the lower extremities reflecting an increased mechanical loading due to their excessive body mass. Even though functional overloading has been associated with increased SLN expression in mice, no significant differences in vastus lateralis SLN protein expression were observed between the overweight and lean subjects. A potential explanation for this finding could be that much larger overloading may be required to increase SLN expression in humans.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast with rodent data, here we have demonstrated that a short training program based on repeated high‐intensity exercise reduced the SLN protein levels in vastus lateralis despite eliciting much less total energy expenditure than the 4‐day walking intervention. A principal difference between prolonged walking and repeated 30‐second all‐out sprints is the marked metabolic disturbances evoked by the sprints, which cause substantial lactic acidosis, oxidative stress, and sarcoplasmic reticulum stress .…”

Section: Discussionmentioning

confidence: 99%

“…Sarcolipin (SLN) is a 31‐amino‐acid protein expressed only in striated and cardiac muscle, which regulates muscle thermogenesis . SLN expression increases with overloading and is associated with enhanced exercise performance in mice . It remains unknown whether, in humans, the expression of SLN is influenced by loading and energy balance.…”

Section: Introductionmentioning

confidence: 99%

“…Comparative analyses of SLN protein expression levels in different human skeletal muscles are lacking. Moreover, functional overloading of plantaris muscle is associated with increased SLN mRNA and protein expression in mice suggesting that chronic overload could stimulate SLN expression. It remains unknown how SLN expression responds to substantially different loading patterns in different human skeletal muscles.…”

Section: Introductionmentioning

confidence: 99%

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Sarcolipin expression in human skeletal muscle: Influence of energy balance and exercise

Morales-Álamo

Martinez‐Canton²,

Gelabert‐Rebato

et al. 2019

Scandinavian Med Sci Sports

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Sarcolipin (SLN) is a SERCA uncoupling protein associated with exercise performance and lower adiposity in mice. To determine SLN protein expression in human skeletal muscle and its relationship with adiposity, resting energy expenditure (REE), and performance, SLN was assessed by Western blot in 199 biopsies from two previous studies. In one study, 15 overweight volunteers underwent a pretest followed by 4 days of caloric restriction and exercise (45‐minute one‐arm cranking + 8‐hour walking), and 3 days on a control diet. Muscle biopsies were obtained from the trained and non‐exercised deltoid, and vastus lateralis (VL). In another study, 16 men performed seven sessions of 4‐6 × 30‐sec all‐out sprints on the cycle ergometer with both limbs, and their VL and triceps brachii biopsied pre‐ and post‐training. SLN expression was twofold and 44% higher in the VL than in the deltoids and triceps brachii, respectively. SLN was associated with neither adiposity nor REE, and was not altered by a severe energy deficit (5500 kcal/day). SLN and cortisol changes after the energy deficit were correlated (r = .38, P = .039). SLN was not altered by low‐intensity exercise in the overweight subjects, whereas it was reduced after sprint training in the other group. The changes in SLN with sprint training were inversely associated with the changes in gross efficiency (r = −.59, P = .016). No association was observed between aerobic or anaerobic performance and SLN expression. In conclusion, sarcolipin appears to play no role in regulating the fat mass of men. Sprint training reduces sarcolipin expression, which may improve muscle efficiency.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sarcolipin expression in human skeletal muscle: Influence of energy balance and exercise

Morales-Álamo

Martinez‐Canton²,

Gelabert‐Rebato

et al. 2019

Scandinavian Med Sci Sports

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“…Western blot analysis was as described ( Riedl et al., 2016 ). Antibodies used are provided in Supplemental Experimental Procedures .…”

Section: Methodsmentioning

confidence: 99%

Human Carboxylesterase 2 Reverses Obesity-Induced Diacylglycerol Accumulation and Glucose Intolerance

et al. 2017

Self Cite

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SummarySerine hydrolases are a large family of multifunctional enzymes known to influence obesity. Here, we performed activity-based protein profiling to assess the functional level of serine hydrolases in liver biopsies from lean and obese humans in order to gain mechanistic insight into the pathophysiology of metabolic disease. We identified reduced hepatic activity of carboxylesterase 2 (CES2) and arylacetamide deacetylase (AADAC) in human obesity. In primary human hepatocytes, CES2 knockdown impaired glucose storage and lipid oxidation. In mice, obesity reduced CES2, whereas adenoviral delivery of human CES2 reversed hepatic steatosis, improved glucose tolerance, and decreased inflammation. Lipidomic analysis identified a network of CES2-regulated lipids altered in human and mouse obesity. CES2 possesses triglyceride and diacylglycerol lipase activities and displayed an inverse correlation with HOMA-IR and hepatic diacylglycerol concentrations in humans. Thus, decreased CES2 is a conserved feature of obesity and plays a causative role in the pathogenesis of obesity-related metabolic disturbances.

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Physiological Regulation of Skeletal Muscle Mass

Pérez-Schindler

Handschin

2019

Nutrition and Skeletal Muscle

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AMPKγ3 is dispensable for skeletal muscle hypertrophy induced by functional overload

Abstract: Riedl I, Osler ME, Björnholm M, Egan B, Nader GA, Chibalin AV, Zierath JR. AMPK␥3 is dispensable for skeletal muscle hypertrophy induced by functional overload.

Cited by 9 publications

References 41 publications

Sarcolipin expression in human skeletal muscle: Influence of energy balance and exercise

Sarcolipin expression in human skeletal muscle: Influence of energy balance and exercise

Human Carboxylesterase 2 Reverses Obesity-Induced Diacylglycerol Accumulation and Glucose Intolerance

Physiological Regulation of Skeletal Muscle Mass

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