AMPK adapts metabolism to developmental energy requirement during dendrite pruning in Drosophila

Marzano, Marco; Herzmann, Svende; Elsbroek, Leonardo; Sanal, Neeraja; Tarbashevich, Katsiaryna; Raz, Erez; Krahn, Michael P.; Rumpf, Sebastian

doi:10.1016/j.celrep.2021.110024

Cited by 16 publications

(17 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to our RNAi test, the decrease in AMPK gene expression inhibited the pediveligers to metamorphose into plantigrades. These results are consistent with previous studies [ 23 , 52 , 53 ]. This suggests that AMPK is required for the larval metamorphosis of mussels, but its specific mechanism in larval metamorphosis is still unclear.…”

Section: Discussionsupporting

confidence: 94%

“…Furthermore, AMPK is in charge of maintaining germline quiescence in Caenorhabditis elegans larvae by changing the germline chromatin landscape to preserve germ cell integrity and regulating larvae metabolism to promote their smooth growth into adults [ 22 ]. When AMPK is activated, it enhances oxidative phosphorylation and the usage of pyruvate and allows Drosophila melanogaster to prune their dendrites of sensory neurons to complete developmental transitions [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

AMPK Promotes Larval Metamorphosis of Mytilus coruscus

Zhang

Wang

et al. 2022

Genes

View full text Add to dashboard Cite

Metamorphosis is a critical process in the transition from planktonic life to benthic life for marine invertebrates, which is accompanied by a large amount of energy consumption. Previous studies have proved that AMP-activated protein kinase (AMPK), as a vital energy regulator, plays a prominent role in mediating the growth and development of terrestrial animals. However, its function in the growth and development of marine invertebrates, especially in metamorphosis, remains elusive. This study explored the function of AMPK in the larval metamorphosis of Mytilus coruscus. The full-length cDNA of AMPK genes in M. coruscus was cloned and characterized, which is composed of three subunits, McAMPKα, McAMPKβ, and McAMPKγ. Pharmacological tests demonstrated that through the application of an AMPK activator, AMP substantially enhanced the larval metamorphosis rate (p < 0.05). By contrast, the larval metamorphosis rate decreased significantly after being treated with the AMPK inhibitor Compound C (p < 0.05). McAMPK gene knock-down resulted in a reduction in McAMPK gene expression (p < 0.05), and the larval metamorphosis of M. coruscus was significantly restrained (p < 0.05). These results indicated that AMPK signaling is vital in the larval metamorphosis of M. coruscus, which advances further understanding in exploring the molecular mechanisms in the metamorphosis of marine invertebrate larvae.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

AMPK Promotes Larval Metamorphosis of Mytilus coruscus

Zhang

Wang

et al. 2022

Genes

View full text Add to dashboard Cite

show abstract

“…Cytoskeletal disassembly occurs in response to ecdysone signaling, but a direct link had previously only been known for actin, as expression of the actin severing enzyme Mical is induced by ecdysone (Kirilly et al, 2009). The kinase regulating microtubule dynamics during pruning, Par-1, is known to be activated by active site phosphorylation, and the Par-1 upstream kinase LKB1 is also required for dendrite pruning (Marzano et al, 2021). In support of a direct effect of ecdysone also on microtubule regulation, we found that ecdysone treatment increased Par-1 levels and active site phosphorylation in biochemical experiments in S2 cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

Developmental pruning of sensory neurites by mechanical tearing in Drosophila

Krämer

Wolterhoff

Galic

et al. 2023

Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

Mechanical forces actively shape cells during development, but little is known about their roles during neuronal morphogenesis. Developmental neurite pruning, a critical circuit specification mechanism, often involves neurite abscission at predetermined sites by unknown mechanisms. Pruning of Drosophila sensory neuron dendrites during metamorphosis is triggered by the hormone ecdysone, which induces local disassembly of the dendritic cytoskeleton. Subsequently, dendrites are severed at positions close to the soma by an unknown mechanism. We found that ecdysone signaling causes the dendrites to become mechanically fragile. Severing occurs during periods of increased pupal morphogenetic tissue movements, which exert mechanical forces on the destabilized dendrites. Tissue movements and dendrite severing peak during pupal ecdysis, a period of strong abdominal contractions, and abolishing ecdysis causes non-cell autonomous dendrite pruning defects. Thus, our data establish mechanical tearing as a novel mechanism during neurite pruning.

show abstract

“…We recently found that the metabolically regulated AMP-dependent protein kinase (AMPK) is required for dendrite pruning in the fly PNS through adaptation of cellular metabolism (31). AMPK is sometimes described as a TOR antagonist, and a recent study found that AMPK also antagonizes TOR during dendrite pruning (32).…”

Section: Discussionmentioning

confidence: 99%

TORC1 regulation of dendrite regrowth after pruning is linked to actin and exocytosis

Sanal

Keding

Gigengack

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Neurite pruning and regrowth are important mechanisms to adapt neural circuits to distinct developmental stages. Neurite regrowth after pruning often depends on differential regulation of growth signaling pathways, but their precise mechanisms of action during regrowth are unclear. Here, we show that the PI3K/TORC1 pathway is required for dendrite regrowth after pruning in Drosophila peripheral neurons during metamorphosis. TORC1 impinges on translation initiation, and our analysis of 5' untranslated regions (UTRs) of remodeling factor mRNAs linked to actin suggests that TOR selectively stimulates the translation of regrowth over pruning factors. Furthermore, we find that dendrite regrowth also requires the GTPase RalA and the exocyst complex as regulators of polarised secretion, and we provide evidence that this pathway is also regulated by TOR. We propose that TORC1 coordinates dendrite regrowth after pruning by coordinately stimulating the translation of regrowth factors involved in cytoskeletal regulation and secretion.

show abstract

AMPK adapts metabolism to developmental energy requirement during dendrite pruning in Drosophila

Cited by 16 publications

References 69 publications

AMPK Promotes Larval Metamorphosis of Mytilus coruscus

AMPK Promotes Larval Metamorphosis of Mytilus coruscus

Developmental pruning of sensory neurites by mechanical tearing in Drosophila

TORC1 regulation of dendrite regrowth after pruning is linked to actin and exocytosis

Contact Info

Product

Resources

About