AMPK Activity Contributes to G2 Arrest and DNA Damage Decrease via p53/p21 Pathways in Oxidatively Damaged Mouse Zygotes

He, Pei; Li, Zhiling; Xu, Feng; Ru, Gaizhen; Huang, Yue; Lin, Emily; Peng, Sanfeng

doi:10.3389/fcell.2020.539485

Cited by 23 publications

(20 citation statements)

References 68 publications

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“…After adding AMPK inhibitor, there was a reversal in G2 phase arrest in mouse fertilized eggs, the rate of blastocysts apoptosis was increased, and the formation rates of four‐cell embryos and blastocysts were decreased. [ 96 ] In summary, evidences indicate that AMPK activation arrests cell cycle and inhibit proliferation of cancer cells.…”

Section: Regulation Mechanisms Of Ampkmentioning

confidence: 99%

“…AMPK is an important gene that regulates the transport of cell energy and controls cell cycle and growth. [ 97 ] It is easy to associate its function with the characteristics of cancer cells. As a tumor suppressor gene, LKB1 can regulate AMPK and at least 12 downstream kinases related to AMPK, [ 75 ] suggesting that AMPK may play an important role in suppressing tumors.…”

Section: The Relationship Between Ampk Activation and Carcinogenesismentioning

confidence: 99%

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Targeting AMPK Signaling by Dietary Polyphenols in Cancer Prevention

Cao

et al. 2021

Molecular Nutrition Food Res

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Cancer is a serious public health problem in the world and a major disease affecting human health. Dietary polyphenols have shown good potential in the treatment of various cancers. It is worth noting that cancer cells usually exhibit metabolic abnormalities of high glucose intake and inefficient utilization. AMPK is the key molecule in the regulation of energy metabolism and is closely related with obesity and diabetes. Recent studies indicate that AMPK also plays an important role in cancer prevention and regulating cancer‐related genes and pathways, and dietary polyphenols can significantly regulate AMPK activity. In this review, the progress of dietary polyphenols preventing carcinogenesis via AMPK pathway is systemically summarized. From the viewpoint of interfering energy metabolism, the anti‐cancer effects of dietary polyphenols are explained. AMPK pathway modulated by different dietary polyphenols affects pathways and target genes are summarized. Dietary polyphenols exert anti‐cancer effect through the target molecules regulated by AMPK, which broadens the understanding of polyphenols anti‐cancer mechanisms and provides value reference for the investigators of the novel field.

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Section: Regulation Mechanisms Of Ampkmentioning

confidence: 99%

Section: The Relationship Between Ampk Activation and Carcinogenesismentioning

confidence: 99%

Targeting AMPK Signaling by Dietary Polyphenols in Cancer Prevention

Cao

et al. 2021

Molecular Nutrition Food Res

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“…We detected the mitochondria membrane potential at 5 hpa, ROS level at 9 hpa and γ-H2A.X at 12 hpa in zygotes, respectively and found that oocyte vitrification decreased the mitochondrial activity and increased ROS production and DNA damage, however, these damages were considerably alleviated by MT treatment, potentially promoting the cleavage rates. The oocyte vitrification-inflicted oxidative stress might delay or arrest the G2/M phase transition via activating ATM/Chk1 [49] or AMPK-p53/p21 [60] pathway, partially leading to delay or arrest of cleavage. It has been reported that MT is a powerful scavenger of ROS and can maintain the genome integrity [62].…”

Section: Discussionmentioning

confidence: 99%

“…It seems that the oxidative stress-induced DNA damage can activate the ATM/Chk1 and phosphorylate Cdc25 and Cdc2, which can further trigger G2/M arrest of zygote [ 49 ]. Furthermore, the oxidative stress-induced phosphorylation and activation of AMPK can inhibit CDK1 activity via p53/p21 pathways, thereby delaying the G2/M phase transition and facilitating the repair of DNA damage in zygotes [ 60 ]. Previously Lei and colleagues have shown that vitrification-inflicted mitochondrial oxidative damage can lead to the reduced developmental potential in vitrified mouse oocytes [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Melatonin improves the first cleavage of parthenogenetic embryos from vitrified–warmed mouse oocytes potentially by promoting cell cycle progression

Pan

Qazi

Guo

et al. 2021

J Animal Sci Biotechnol

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Background This study investigated the effect of melatonin (MT) on cell cycle (G1/S/G2/M) of parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II (MII) oocytes and elucidated the potential mechanism of MT action in the first cleavage of embryos. Results After vitrification and warming, oocytes were parthenogenetically activated (PA) and in vitro cultured (IVC). Then the spindle morphology and chromosome segregation in oocytes, the maternal mRNA levels of genes including Miss, Doc1r, Setd2 and Ythdf2 in activated oocytes, pronuclear formation, the S phase duration in zygotes, mitochondrial function at G1 phase, reactive oxygen species (ROS) level at S phase, DNA damage at G2 phase, early apoptosis in 2-cell embryos, cleavage and blastocyst formation rates were evaluated. The results indicated that the vitrification/warming procedures led to following perturbations 1) spindle abnormalities and chromosome misalignment, alteration of maternal mRNAs and delay in pronucleus formation, 2) decreased mitochondrial membrane potential (MMP) and lower adenosine triphosphate (ATP) levels, increased ROS production and DNA damage, G1/S and S/G2 phase transition delay, and delayed first cleavage, and 3) increased early apoptosis and lower levels of cleavage and blastocyst formation. Our results further revealed that such negative impacts of oocyte cryopreservation could be alleviated by supplementation of warming, recovery, PA and IVC media with 10− 9 mol/L MT before the embryos moved into the 2-cell stage of development. Conclusions MT might promote cell cycle progression via regulation of MMP, ATP, ROS and maternal mRNA levels, potentially increasing the first cleavage of parthenogenetic zygotes developed from vitrified–warmed mouse oocytes and their subsequent development.

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“…This situation is reflected in this study where different genes related to oxidative stress were upregulated in IVP embryos, including essential genes involved in the protection of cells against free radicals such as GSS , GPX4 , GSTA , MGST1 [ 67 ], TXN , TXNIP , NXN [ 68 ], SOD1 [ 69 ] and SESN1 [ 70 ]. One of the main consequences of this overexposure to different types of free radicals is the damage that they cause to DNA [ 71 ], which has been confirmed in both oocytes [ 72 ] and embryos [ 73 , 74 , 75 ]. In that sense, in this study, we observed how, independent of the culture type, IVP embryos overexpressed genes involved in DNA repair processes.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Profiling of Porcine Blastocysts Produced In Vitro in a Chemically Defined Culture Medium

Cambra

Martı́nez

Rodriguez‐Martinez

et al. 2021

Animals

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The development of chemically defined media is a growing trend in in vitro embryo production (IVP). Recently, traditional undefined culture medium with bovine serum albumin (BSA) has been successfully replaced by a chemically defined medium using substances with embryotrophic properties such as platelet factor 4 (PF4). Although the use of this medium sustains IVP, the impact of defined media on the embryonic transcriptome has not been fully elucidated. This study analyzed the transcriptome of porcine IVP blastocysts, cultured in defined (PF4 group) and undefined media (BSA group) by microarrays. In vivo-derived blastocysts (IVV group) were used as a standard of maximum embryo quality. The results showed no differentially expressed genes (DEG) between the PF4 and BSA groups. However, a total of 2780 and 2577 DEGs were detected when comparing the PF4 or the BSA group with the IVV group, respectively. Most of these genes were common in both in vitro groups (2132) and present in some enriched pathways, such as cell cycle, lysosome and/or metabolic pathways. These results show that IVP conditions strongly affect embryo transcriptome and that the defined culture medium with PF4 is a guaranteed replacement for traditional culture with BSA.

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AMPK Activity Contributes to G2 Arrest and DNA Damage Decrease via p53/p21 Pathways in Oxidatively Damaged Mouse Zygotes

Cited by 23 publications

References 68 publications

Targeting AMPK Signaling by Dietary Polyphenols in Cancer Prevention

Targeting AMPK Signaling by Dietary Polyphenols in Cancer Prevention

Melatonin improves the first cleavage of parthenogenetic embryos from vitrified–warmed mouse oocytes potentially by promoting cell cycle progression

Transcriptional Profiling of Porcine Blastocysts Produced In Vitro in a Chemically Defined Culture Medium

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