Amphotericin B-Loaded Poly(lactic-co-glycolic acid) Nanofibers: An Alternative Therapy Scheme for Local Treatment of Vulvovaginal Candidiasis

Souza, Ramon Oliveira; Lima, Tadeu Henrique; Oréfice, Rodrigo Lambert; Araújo, Marcelo Gonzaga de Freitas; Moura, Sandra Aparecida de Lima; Magalhães, Juliana Teixeira de; Silva, Gisele Rodrigues da

doi:10.1016/j.xphs.2018.06.017

Cited by 22 publications

(10 citation statements)

References 36 publications

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“…Histological analysis in rats revealed the normal appearance of the kidney and heart, indicating the absence of nephrotoxicity and cardiotoxicity with intravaginal application of AmB-loaded PLGA nanofibers. These findings demonstrate the potential of PLGA nanofibers as an alternative scheme for treatment of VVC that ensure patient compliance [88].…”

Section: Vaginal Nanofibersmentioning

confidence: 64%

Promising Drug Delivery Approaches to Treat Microbial Infections in the Vagina: A Recent Update

et al. 2020

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An optimal host–microbiota interaction in the human vagina governs the reproductive health status of a woman. The marked depletion in the beneficial Lactobacillus sp. increases the risk of infection with sexually transmitted pathogens, resulting in gynaecological issues. Vaginal infections that are becoming increasingly prevalent, especially among women of reproductive age, require an effective concentration of antimicrobial drugs at the infectious sites for complete disease eradication. Thus, topical treatment is recommended as it allows direct therapeutic action, reduced drug doses and side effects, and self-insertion. However, the alterations in the physiological conditions of the vagina affect the effectiveness of vaginal drug delivery considerably. Conventional vaginal dosage forms are often linked to low retention time in the vagina and discomfort which significantly reduces patient compliance. The lack of optimal prevention and treatment approaches have contributed to the unacceptably high rate of recurrence for vaginal diseases. To combat these limitations, several novel approaches including nano-systems, mucoadhesive polymeric systems, and stimuli-responsive systems have been developed in recent years. This review discusses and summarises the recent research progress of these novel approaches for vaginal drug delivery against various vaginal diseases. An overview of the concept and challenges of vaginal infections, anatomy and physiology of the vagina, and barriers to vaginal drug delivery are also addressed.

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Section: Vaginal Nanofibersmentioning

confidence: 64%

Promising Drug Delivery Approaches to Treat Microbial Infections in the Vagina: A Recent Update

et al. 2020

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“…No resistance was evidenced in the in vitro model and, in vivo, the drug eliminated the fungal burden after three days of treatment. These results suggest that the use of ABloaded poly (lactic-co-glycolic acid) nanofibers may represent a strategy for local treatment without the induction of fungal resistance, assuring the adhesion and safety to the patient 20 .…”

Section: Mechanisms Of Resistancementioning

confidence: 89%

Nephrotoxicity and fungal resistance associated with Amphotericin B

Berto¹,

Dalzochio²

2021

Saúde e meio ambient.: rev. interdisciplin.

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Introduction: Amphotericin B (AB) is the drug of choice for the treatment of several systemic fungal infections. Despite of its effectiveness and minimum relation to the resistance, AB is toxic to animal cells, in particular to the kidney cells. Objective: To address the aspects of resistance, nephrotoxicity and biomarkers available for the early detection of kidney disease caused by AB. Method: The bibliographic search was performed in online databases. Papers published between 2008-2019, were selected for further analysis and inclusion in the present review. Results: The search resulted in 42 eligible papers which the main findings were: (i) the frequency of resistance to AB appears to be substantial, however, some species should be carefully studied given their diminished susceptibility; (ii) regarding nephrotoxicity, new techniques and methods have promisingly been implemented; and (iii) a novel generation of biomarkers for kidney injury is more specific and sensitive. Conclusion: The adoption of conjugated drug delivery systems is a consistent alternative to minimize nephrotoxicity and fungal resistance. Considering the biomarkers, it is more likely that, instead of a punctual assessment of a single biomarker, a set of biomarkers provides a more thorough comprehension of the aspects related to AB-induced kidney injury. Nonetheless, more clinical studies are necessary for further implementation of such biomarkers in the clinical routine. Keywords: Amphotericin B. Nephrotoxicity. Acute Kidney Injury. Biomarkers.

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“…However, for this test, all AmpB was available through all the period evaluated, unlike nanofibers in which the release occurred in smaller quantities at the same time, which was the factor that did not allow the formation of larger inhibition zone. Also, under dynamic conditions these nanofibers would promote a constant release, for a longer period favoring the inhibition of microrganisms, presenting itself as an effective measure against disease recurrence (Souza et al, 2018). Thus, nanofibers can act as a potential drug delivery system to model drug AmpB, and could be promising for the treatment of leishmaniasis and severe fungal infections (Nanda and Mishra, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…However, unwanted characteristics of AmpB, including high cost, toxicity, and necessity of parenteral administration, may limit its use (Mattos et al, 2015;Turner et al, 2015;Kariyawasam et al, 2019). For this reason, other vehicles have been developed with the aim of developing new strategies for the administration of this drug, such as films, nanofibers, nanoformulations, as well as systems focused on delivery to the oral mucosa (Hodiamont et al, 2015;Sarwar et al, 2018;Souza et al, 2018;Edmans et al, 2020). Among these vehicles, nanocomposites are drug delivery systems, whose formulation with different associated polymers may be able to optimize the properties of the controlled release of drugs, with different applications, among them, films for wound healing (Hasan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Core-Shell Nanofibers Incorporating Amphotericin B by Solution Blow Spinning Against Leishmania and Candida Species

Gonçalves

Rocha

Pires

et al. 2020

Front. Bioeng. Biotechnol.

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The aim of this study was to develop polymeric nanofibers for controlled administration of Amphotericin B (AmpB), using the solution centrifugation technique, characterizing its microstructural and physical properties, release rate, and activity against Leishmania and Candida species. The core-shell nanofibers incorporated with AmpB were synthesized by Solution Blow Spinning (SBS) and characterized by scanning electron microscopy (SEM), differential scanning calorimetry, X-Ray diffraction, and drug release assay. In vitro leishmanicidal and antifungal activity were also evaluated. Fibrous membranes with uniform morphology and smooth surfaces were produced. The intensity of the diffraction peaks becomes slightly more pronounced, assuming the increased crystallization in PLA/PEG at high AmpB loadings. Drug release occurred and the solutions with nanofibers to encourage greater incorporation of AmpB showed a higher concentration. In the results of the experiment with promastigotes, the wells treated with nanofibers containing concentrations of AmpB at 0.25, 0.5, and 1%, did not have any viable cells, similar to the positive control. Various concentrations of AmpB improved the inhibition of fungal growth. The delivery system based on PLA/PEG nanofibers was properly developed for AmpB, presenting a controlled release and a successful encapsulation, as well as antifungal and antileishmanial activity.

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Amphotericin B-Loaded Poly(lactic-co-glycolic acid) Nanofibers: An Alternative Therapy Scheme for Local Treatment of Vulvovaginal Candidiasis

Cited by 22 publications

References 36 publications

Promising Drug Delivery Approaches to Treat Microbial Infections in the Vagina: A Recent Update

Promising Drug Delivery Approaches to Treat Microbial Infections in the Vagina: A Recent Update

Nephrotoxicity and fungal resistance associated with Amphotericin B

Effectiveness of Core-Shell Nanofibers Incorporating Amphotericin B by Solution Blow Spinning Against Leishmania and Candida Species

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