Amphoteric nano-, micro-, and macrogels, membranes, and thin films

Kudaibergenov, Sarkyt E.; Nuraje, Nurxat; Khutoryanskiy, Vitaliy V.

doi:10.1039/c2sm25766a

Cited by 79 publications

(62 citation statements)

References 211 publications

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“…7 The numerous applications of stimuli-sensitive systems based on (micro)gels provide reversible switching between interacting and noninteracting states of biomaterial for a number of cycles (cell culture, 8 drug delivery, 9 etc.). Reversible temperature-induced change in specific enzymatic activity has also been demonstrated for (micro)gels comprising immobilized enzymes.…”

Section: ■ Introductionmentioning

confidence: 99%

Dual-Stimuli-Sensitive Microgels as a Tool for Stimulated Spongelike Adsorption of Biomaterials for Biosensor Applications

et al. 2014

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This work examines the fabrication regime and the properties of microgel and microgel/enzyme thin films adsorbed onto conductive substrates (graphite or gold). The films were formed via two sequential steps: the adsorption of a temperature- and pH-sensitive microgel synthesized by precipitation copolymerization of N-isopropylacrylamide (NIPAM) and 3-(N,N-dimethylamino)propylmethacrylamide (DMAPMA) (poly(NIPAM-co-DMAPMA) at the pH-condition corresponding to its noncharged state (first step of adsorption), followed by the enzyme, tyrosinase, adsorption at the pH-condition when the microgel and the enzyme are oppositely charged (second step of adsorption). The stimuli-sensitive properties of poly(NIPAM-co-DMAPMA) microgel were characterized by potentiometric titration and dynamic light scattering (DLS) in solution as well as by atomic force microscopy (AFM) and quartz crystal microbalance with dissipation monitoring (QCM-D) at solid interface. Enhanced deposition of poly(NIPAM-co-DMAPMA) microgel particles was shown at elevated temperatures exceeding the volume phase transition temperature (VPTT). The subsequent electrostatic interaction of the poly(NIPAM-co-DMAPMA) microgel matrix with tyrosinase was examined at different adsorption regimes. A considerable increase in the amount of the adsorbed enzyme was detected when the microgel film is first brought into a collapsed state but then was allowed to interact with the enzyme at T < VPTT. Spongelike approach to enzyme adsorption was applied for modification of screen-printed graphite electrodes by poly(NIPAM-co-DMAPMA)/tyrosinase films and the resultant biosensors for phenol were tested amperometrically. By temperature-induced stimulating both (i) poly(NIPAM-co-DMAPMA) microgel adsorption at T > VPTT and (ii) following spongelike tyrosinase loading at T < VPTT, we can achieve more than 3.5-fold increase in biosensor sensitivity for phenol assay. Thus, a very simple, novel, and fast strategy for physical entrapment of biomolecules by the polymeric matrix was proposed and tested. Being based on this unique stimuli-sensitive behavior of the microgel, this stimulated spongelike adsorption provides polymer films comprising concentrated biomaterial.

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Section: ■ Introductionmentioning

confidence: 99%

Dual-Stimuli-Sensitive Microgels as a Tool for Stimulated Spongelike Adsorption of Biomaterials for Biosensor Applications

et al. 2014

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“…Ampholytic ionomers contain both acidic and basic groups in a single macromolecule . This type of polyelectrolyte has been known for a long time and amphoteric, permselective separation membranes were first described in 1975 .…”

Section: Introductionmentioning

confidence: 99%

Low‐Permeability Poly(ether Ether Ketone)‐Based Ampholytic Membranes

et al. 2016

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Ion-exchange membranes based on sulfonated and sulfaminated poly(ether ether ketone) were prepared by a modified sulfamination route. In a first step, poly(ether ether ketone) was sulfonated. The sulfonic acid groups were then transformed into chlorosulfonic moieties by reaction with thionyl chloride. A proportion of the chlorosulfonic functionalities reacted with dimethylamine to give basic sulfonamide groups, whereas those remaining were hydrolyzed back to sulfonate moieties obtaining, after immersion in acidic solution, an ampholytic polymer. The thermal, mechanical, electrical, and permeability properties of these amphoteric membranes were characterized. These membranes exhibit good thermal and mechanical stability and ultra-low vanadium ion permeability. The type and value of ion conductivity can be adjusted by the choice of acidic or basic medium. The tunable ion conductivity and the low ion permeability are suitable characteristics for the development of high-performance separator membranes

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“…The classic observable changes utilized are either colorimetric or fluorometric, although sensors based upon a gelation response [11] or other physical state changes have also been developed. [12, 13] Chiroptical changes measured by circular dichroism (CD) enhancement is emerging as a new method for analyte detection. [14] Fluorescence remains the most commonly utilized spectroscopic change because of the enhanced sensitivity compared to colorimetric changes.…”

Section: Sensing Strategiesmentioning

confidence: 99%

Ion and Molecular Recognition Using Aryl–Ethynyl Scaffolding

Vonnegut

Tresca

Johnson

et al. 2015

Chemistry An Asian Journal

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The aryl–ethynyl linkage has been extensively employed in the construction of hosts for a variety of guests. Uses range from ion detection (e.g., of metal cations in the environment or industrial waste and of anions prevalent in nature), to molecular mimics for biological systems, and to applications targeting future safety issues (such as CO2 capture and indicators for the manufacture of chemical weapons). This Focus Review examines the utilization of the aryl–ethynyl linkage in engineering host molecules for a variety of different guests, and how the alkyne unit plays an integral part as both a rigid scaffolding section in host geometry design as well as a linker to allow conjugative communication between discrete π-electron systems.

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Amphoteric nano-, micro-, and macrogels, membranes, and thin films

Cited by 79 publications

References 211 publications

Dual-Stimuli-Sensitive Microgels as a Tool for Stimulated Spongelike Adsorption of Biomaterials for Biosensor Applications

Dual-Stimuli-Sensitive Microgels as a Tool for Stimulated Spongelike Adsorption of Biomaterials for Biosensor Applications

Low‐Permeability Poly(ether Ether Ketone)‐Based Ampholytic Membranes

Ion and Molecular Recognition Using Aryl–Ethynyl Scaffolding

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