Amphiphilic Polymethyloxazoline–Polyethyleneimine Copolymers: Interaction with Lipid Bilayer and Antibacterial Properties

Kozon, Dominika; Mierzejewska, Jolanta; Kobiela, Tomasz; Grochowska, Agnieszka; Dudnyk, Ksenia; Głogowska, Agnieszka; Sobiepanek, Anna; Kuźmińska, Aleksandra; Ciach, Tomasz; Augustynowicz–Kopeć, Ewa; Jańczewski, Dominik

doi:10.1002/mabi.201900254

Cited by 17 publications

(23 citation statements)

References 77 publications

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“…[28] Among structurally similar molecular entities, antimicrobial copolymers with higher zeta potentials typically have more potent bactericidal activity than those with lower zeta potentials. [29] In consistence with these observations, our experiments show that the antimicrobial activity of bactericidal histone is declined after combining with NET DNA (Figure 3c). [19,29] The number of floating bacteria decreases as the fraction of histone in μwebs increases, likely for two reasons: either histone facilitates the killing of planktonic S. aureus, and/or the histone-induced environmental stress promotes conversion from planktonic state to The model of electrostatic interactions between μwebs and S. aureus is further supported by our zeta potential measurements.…”

Section: Resultssupporting

confidence: 79%

“…[29] In consistence with these observations, our experiments show that the antimicrobial activity of bactericidal histone is declined after combining with NET DNA (Figure 3c). [19,29] The number of floating bacteria decreases as the fraction of histone in μwebs increases, likely for two reasons: either histone facilitates the killing of planktonic S. aureus, and/or the histone-induced environmental stress promotes conversion from planktonic state to The model of electrostatic interactions between μwebs and S. aureus is further supported by our zeta potential measurements. After the DNA (ζ = −55.9 ± 2.4 mV) and histone (ζ = +11.6 ± 0.3 mV) solutions are mixed to form μweb suspension, the zeta potential of resultant μwebs increases with the growing histone fraction (see Figure S4, Supporting Information).…”

Section: Resultssupporting

confidence: 79%

“…[29] In consistence with these observations, our experiments show that the antimicrobial activity of bactericidal histone is declined after combining with NET DNA (Figure 3c). [19,29] The number of floating bacteria decreases as the fraction of histone in μwebs increases, likely for two reasons: either histone facilitates the killing of planktonic S. aureus, and/or the histone-induced environmental stress promotes conversion from planktonic state to biofilm. Adhesion of S. aureus on μwebs decreases as the DNA fraction in the μwebs increases, probably driven by the growing electrostatic repulsion between bacterial matrix proteins and DNA phosphate backbones, which in turn attenuates the antimicrobial potency of μwebs against floating bacteria.…”

Section: Resultssupporting

confidence: 79%

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Dosage‐Dependent Antimicrobial Activity of DNA‐Histone Microwebs Against Staphylococcus Aureus

Yang

Shi

Ahmed

et al. 2021

Adv Materials Inter

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Biofilms are surface-bound microbe assemblies encased in an extracellular matrix including extracellular DNA (eDNA), proteins, and polysaccharides, which allow bacteria to resist host immune clearing mechanisms. [1] One critical biofilm component is eDNA, which helps maintain biofilm structural integrity, [2] as well as counter host antimicrobial peptides [3] and antibiotic treatments. [4] Although eDNA can engage positively with pathogens, it is known to have an important role in the innate immune response, where neutrophils may be triggered to release neutrophil extracellular traps (NETs), [5,6] cobweb-structured DNA-histone complexes that capture and disarm microorganisms. [7,8] The observed antimicrobial activity of NET-based eDNA is due, at least in part, to their chelation with bacterial membrane proteins and subsequent lysis. [9] Histones, key NET components that modulate the self-assembly of DNA, also contribute to overall antimicrobial activity by destabilizing bacterial cell walls and chromosome organization. [10,11] It is also reported that histone inhibits biofilm formation in a dosage-dependent manner. [12] Previous studies have indicated that the antimicrobial activity of DNA or histone largely depends on their surface charges, and blockage of the surface charge results in declined antimicrobial activity. [9,11] Staphylococcus aureus is an opportunistic pathogen that can cause persistent biofilm infections in soft tissues and bones. [13] Planktonic S. aureus induces neutrophils to produce NETs that can effectively trap and suppress the bacteria. [7] On the other hand, S. aureus biofilms can thwart the antimicrobial functions of NETs by secreting nucleases to degrade the eDNA in NETs; [5,14,15] they also utilize fibronectin-binding protein B (FnBPB) and proteases to neutralize histone-mediated killing. [10,16] Producing DNA-degrading nucleases that protect them from NETs within hours of surface attachment, [5] S. aureus biofilms actively induce neutrophil NETosis to protect themselves from other granulocytic killing mechanisms. [17] In contrast to the many studies that study how biofilms affect neutrophils, NETosis, and NETs, there is no systematic analysis of how different types of NETs may impact planktonic S. aureus response in forming biofilms. This may be important because NET composition including protein content and DNA sources Neutrophil extracellular traps (NETs) are antimicrobial cobweb-structured materials produced by immune cells for clearance of pathogens in the body, but are paradoxically associated with biofilm formation and exacerbated lung infections. To provide a better materials perspective on the pleiotropic roles played by NETs at diverse compositions/concentrations, a NETs-like material (called "microwebs", abbreviated as μwebs) is synthesized for decoding the antimicrobial activity of NETs against Staphylococcus aureus in infection-relevant conditions. It is shown that μwebs composed of low-to-intermediate concentrations of DNA-histone complexes successfully trap and inhibit S...

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Section: Resultssupporting

confidence: 79%

Section: Resultssupporting

confidence: 79%

“…[29] In consistence with these observations, our experiments show that the antimicrobial activity of bactericidal histone is declined after combining with NET DNA (Figure 3c). [19,29] The number of floating bacteria decreases as the fraction of histone in μwebs increases, likely for two reasons: either histone facilitates the killing of planktonic S. aureus, and/or the histone-induced environmental stress promotes conversion from planktonic state to biofilm. Adhesion of S. aureus on μwebs decreases as the DNA fraction in the μwebs increases, probably driven by the growing electrostatic repulsion between bacterial matrix proteins and DNA phosphate backbones, which in turn attenuates the antimicrobial potency of μwebs against floating bacteria.…”

Section: Resultssupporting

confidence: 79%

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Dosage‐Dependent Antimicrobial Activity of DNA‐Histone Microwebs Against Staphylococcus Aureus

Yang

Shi

Ahmed

et al. 2021

Adv Materials Inter

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“…The difference in the mitochondrial dehydrogenase activity of living cells was studied by the MTT assay as described previously [37]. Cells were seeded in a 96-well plate at the density of 1 × 10 4 cells/well.…”

Section: Mtt Assaymentioning

confidence: 99%

Anandamide-Modulated Changes in Metabolism, Glycosylation Profile and Migration of Metastatic Melanoma Cells

Sobiepanek

Milner-Krawczyk

Musolf

et al. 2022

Cancers

Self Cite

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An effective therapy for advanced melanoma, a skin cancer with the highest mortality, has not yet been developed. The endocannabinoid system is considered to be an attractive target for cancer treatment. The use of endocannabinoids, such as anandamide (AEA), is considered to be much greater than as a palliative agent. Thus, we checked its influence on various signaling pathways in melanoma cells. Our investigation was performed on four commercial cell lines derived from different progression stages (radial WM35 and vertical WM115 growth phases, lymph node WM266-4 metastasis, solid tumor A375-P metastasis). Cell viability, glucose uptake, quantification of reactive oxygen species production, expression of selected genes encoding glycosyltransferases, quantification of glycoproteins production and changes in the glycosylation profile and migration, as well as in cell elastic properties were analyzed. The cell glycosylation profile was investigated using the biophysical profiling method—the quartz crystal microbalance with dissipation monitoring (QCM-D). Anandamide treatment of only metastatic cells resulted in: an increase in the cell metabolism, a decrease in GFAT-1 and DPM1 expression, followed by a decrease in L1-CAM glycoprotein production, which further influenced the reduction in the cell glycosylation profile and migration. Considering our results, AEA usage is highly recommended in the combined therapy of advanced melanoma.

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“…This question is intensively studied on the biomembrane models represented by liposomes, bilayer lipid membranes (BLM), and lipid monolayers [ 23 , 24 , 25 ]. These factors were discussed for antibacterial PEI-PMOX copolymers, and also for model lipid bilayers [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity in Lateral Distribution of Polycations at the Surface of Lipid Membrane: From the Experimental Data to the Theoretical Model

2021

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Natural and synthetic polycations of different kinds attract substantial attention due to an increasing number of their applications in the biomedical industry and in pharmacology. The key characteristic determining the effectiveness of the majority of these applications is the number of macromolecules adsorbed on the surface of biological cells or their lipid models. Their study is complicated by a possible heterogeneity of polymer layer adsorbed on the membrane. Experimental methods reflecting the structure of the layer include the electrokinetic measurements in liposome suspension and the boundary potential of planar bilayer lipid membranes (BLM) and lipid monolayers with a mixed composition of lipids and the ionic media. In the review, we systematically analyze the methods of experimental registration and theoretical description of the laterally heterogeneous structures in the polymer layer published in the literature and in our previous studies. In particular, we consider a model based on classical theory of the electrical double layer, used to analyze the available data of the electrokinetic measurements in liposome suspension with polylysines of varying molecular mass. This model suggests a few parameters related to the heterogeneity of the polymer layer and allows determining the conditions for its appearance at the membrane surface. A further development of this theoretical approach is discussed.

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Amphiphilic Polymethyloxazoline–Polyethyleneimine Copolymers: Interaction with Lipid Bilayer and Antibacterial Properties

Cited by 17 publications

References 77 publications

Dosage‐Dependent Antimicrobial Activity of DNA‐Histone Microwebs Against Staphylococcus Aureus

Dosage‐Dependent Antimicrobial Activity of DNA‐Histone Microwebs Against Staphylococcus Aureus

Anandamide-Modulated Changes in Metabolism, Glycosylation Profile and Migration of Metastatic Melanoma Cells

Heterogeneity in Lateral Distribution of Polycations at the Surface of Lipid Membrane: From the Experimental Data to the Theoretical Model

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