Amphiphilic metabolites in gallbladder bile: Potential biomarkers for gallbladder diseases

Ko, Hyeji; Choi, Ikjang; Chang, Kyeol; Jeong, Gijin; Gong, Gyeonghyeon; Seo, Hyeonglim; Ryu, Donghyun; Lee, Kyeong Geun; Choi, Dongho; Chung, Hoeil; Lee, Youngbok

doi:10.1080/05704928.2016.1167707

Cited by 7 publications

(6 citation statements)

References 39 publications

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“…The highest number of sterol, bile acid, and acylcarnitine profiles were found in the pig bile samples, and they differed from the other biological samples analyzed. Bile is mainly constituted by bile acids, cholesterol, phospholipids, and proteins [45]. Clearly, the pig bile analyzed in our study showed clear signals of many glyco-and tauro-conjugated bile acids, and cholesterol.…”

Section: Sterol/bile Acid/acylcarnitine Profiles In Bile Samplesmentioning

confidence: 56%

Identification and Distribution of Sterols, Bile Acids, and Acylcarnitines by LC–MS/MS in Humans, Mice, and Pigs—A Qualitative Analysis

et al. 2022

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Sterols, bile acids, and acylcarnitines are key players in human metabolism. Precise annotations of these metabolites with mass spectrometry analytics are challenging because of the presence of several isomers and stereoisomers, variability in ionization, and their relatively low concentrations in biological samples. Herein, we present a sensitive and simple qualitative LC–MS/MS (liquid chromatography with tandem mass spectrometry) method by utilizing a set of pure chemical standards to facilitate the identification and distribution of sterols, bile acids, and acylcarnitines in biological samples including human stool and plasma; mouse ileum, cecum, jejunum content, duodenum content, and liver; and pig bile, proximal colon, cecum, heart, stool, and liver. With this method, we detected 24 sterol, 32 bile acid, and 27 acylcarnitine standards in one analysis that were separated within 13 min by reversed-phase chromatography. Further, we observed different sterol, bile acid, and acylcarnitine profiles for the different biological samples across the different species. The simultaneous detection and annotation of sterols, bile acids, and acylcarnitines from reference standards and biological samples with high precision represents a valuable tool for screening these metabolites in routine scientific research.

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Section: Sterol/bile Acid/acylcarnitine Profiles In Bile Samplesmentioning

confidence: 56%

Identification and Distribution of Sterols, Bile Acids, and Acylcarnitines by LC–MS/MS in Humans, Mice, and Pigs—A Qualitative Analysis

et al. 2022

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“…Therefore, an increasing number of metabolomics studies have focused on the bile acids profile in gallstones, and demonstrate that the levels of some bile acids are significantly associated with the development of gallstones [19][20][21]. However, there is still lack of a consistent and comprehensive conclusion and the biological samples they detect are diverge.…”

Section: Plos Onementioning

confidence: 99%

Predictive value of bile acids as metabolite biomarkers for gallstones: A protocol of systematic review and meta-analysis

Han

Wang

et al. 2023

PLoS ONE

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Background Plenty of studies have focused on the bile acids profile in gallstones. The objective of our systematic review is to provide a comprehensive summary about bile acids profiles in gallstones and analyzes the difference between gallstones and control group in diverse samples, determining the characteristic bile acids as the metabolite biomarkers for predicting gallstone. Methods EMBASE, the Cochrane Library, PubMed, Web of Science, Wanfang databases, China National Knowledge Infrastructure (CNKI), VIP Information Resource Integration Service Platform (CQVIP), and China Biology Medicine Disc (SinoMed) will be searched with the keywords of gallstones and metabolomics. The screening process will be performed strictly according to inclusion and exclusion criteria. The CONSORT checklist and the Newcastle-Ottawa Scale (NOS) will assess the risk of bias for randomized controlled trials and observational studies, respectively. The qualitative review will be conducted to summarize the bile acids profile in gallstones. The concentrations of bile acids in both case group and control group will be the primary outcomes to perform the meta-analyses. Expected results Our systematic review will find the characteristic bile acids as the candidate metabolite biomarkers which equipped potential value to predict gallstones. Conclusion Expanding the current knowledge on the physiopathology of gallstones and identifying novel predictive biomarkers can help to facilitate the detection and management of gallstones. Consequently, we expect this protocol to be a reasonable method to filtrate candidate differential bile acids which have potential value to predict gallstones. PROSPERO registration number CRD42022339649.

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“…NMR technology has also provided useful information about differences in metabolite concentrations among chronic cholecystitis, xanthogranulomatous cholecystitis, and gallbladder cancer patients (GBC) [225]. Cholic acid can be used to differentiate between different types of gallbladder diseases [226]. Extracts of gallstones with GBC present higher concentrations of calcium and magnesium compared to non-cancerous gallbladder inflammation [227].…”

Section: Omic Approaches Applied To the Study Of Gallbladder Metageno...mentioning

confidence: 99%

The Role of the Gallbladder, the Intestinal Barrier and the Gut Microbiota in the Development of Food Allergies and Other Disorders

Abril

Villa

Sánchez-Pérez

et al. 2022

IJMS

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The microbiota present in the gastrointestinal tract is involved in the development or prevention of food allergies and autoimmune disorders; these bacteria can enter the gallbladder and, depending on the species involved, can either be benign or cause significant diseases. Occlusion of the gallbladder, usually due to the presence of calculi blocking the bile duct, facilitates microbial infection and inflammation, which can be serious enough to require life-saving surgery. In addition, the biliary salts are secreted into the intestine and can affect the gut microbiota. The interaction between the gut microbiota, pathogenic organisms, and the human immune system can create intestinal dysbiosis, generating a variety of syndromes including the development of food allergies and autoimmune disorders. The intestinal microbiota can aggravate certain food allergies, which become severe when the integrity of the intestinal barrier is affected, allowing bacteria, or their metabolites, to cross the intestinal barrier and invade the bloodstream, affecting distal body organs. This article deals with health conditions and severe diseases that are either influenced by the gut flora or caused by gallbladder obstruction and inflammation, as well as putative treatments for those illnesses.

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Amphiphilic metabolites in gallbladder bile: Potential biomarkers for gallbladder diseases

Cited by 7 publications

References 39 publications

Identification and Distribution of Sterols, Bile Acids, and Acylcarnitines by LC–MS/MS in Humans, Mice, and Pigs—A Qualitative Analysis

Identification and Distribution of Sterols, Bile Acids, and Acylcarnitines by LC–MS/MS in Humans, Mice, and Pigs—A Qualitative Analysis

Predictive value of bile acids as metabolite biomarkers for gallstones: A protocol of systematic review and meta-analysis

The Role of the Gallbladder, the Intestinal Barrier and the Gut Microbiota in the Development of Food Allergies and Other Disorders

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